A feeding induced switch from a variable to a homogenous state of the earthworm gut microbiota within a host population.

<h4>Background</h4>The distribution pattern of the earthworm gut microbiota at the host population level is of fundamental importance to understand host-microbiota interactions. Our current understanding of these interactions is very limited. Since feeding represents a main perturbation...

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Autores principales:	Knut Rudi, Kristin Odegård, Tine Therese Løkken, Robert Wilson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f5b696038d0245ab8fe9eb86fd34ca22
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spelling	oai:doaj.org-article:f5b696038d0245ab8fe9eb86fd34ca222021-11-25T06:28:38ZA feeding induced switch from a variable to a homogenous state of the earthworm gut microbiota within a host population.1932-620310.1371/journal.pone.0007528https://doaj.org/article/f5b696038d0245ab8fe9eb86fd34ca222009-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19841743/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The distribution pattern of the earthworm gut microbiota at the host population level is of fundamental importance to understand host-microbiota interactions. Our current understanding of these interactions is very limited. Since feeding represents a main perturbation of the gut microbiota, we determined the effect of a single dose of feed on the microbiota associated with an earthworm population in a simulated microenvironment.<h4>Methodology</h4>Earthworms were sampled 0, 1 and 7 days after feeding. We determined the overall composition of the earthworm-associated microbiota by 16S rRNA gene cloning and sequencing. Based on the 16S rRNA gene data we constructed quantitative PCR's (Q-PCR) for the seven most dominating bacterial groups.<h4>Principal findings</h4>Q-PCR revealed low density and highly variable microbiota among the earthworms before feeding, while a high-density homologous microbiota resulted from feeding. We found that the microbiota 1 day after feeding was more equal to the microbiota after 7 days than before feeding. Furthermore, we found that the gut microbiota was very distinct from that of the bedding and the feed.<h4>Significance</h4>The homogenous population response represents fundamental new knowledge about earthworm gut associated bacteria.Knut RudiKristin OdegårdTine Therese LøkkenRobert WilsonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 10, p e7528 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Knut Rudi Kristin Odegård Tine Therese Løkken Robert Wilson A feeding induced switch from a variable to a homogenous state of the earthworm gut microbiota within a host population.
description	<h4>Background</h4>The distribution pattern of the earthworm gut microbiota at the host population level is of fundamental importance to understand host-microbiota interactions. Our current understanding of these interactions is very limited. Since feeding represents a main perturbation of the gut microbiota, we determined the effect of a single dose of feed on the microbiota associated with an earthworm population in a simulated microenvironment.<h4>Methodology</h4>Earthworms were sampled 0, 1 and 7 days after feeding. We determined the overall composition of the earthworm-associated microbiota by 16S rRNA gene cloning and sequencing. Based on the 16S rRNA gene data we constructed quantitative PCR's (Q-PCR) for the seven most dominating bacterial groups.<h4>Principal findings</h4>Q-PCR revealed low density and highly variable microbiota among the earthworms before feeding, while a high-density homologous microbiota resulted from feeding. We found that the microbiota 1 day after feeding was more equal to the microbiota after 7 days than before feeding. Furthermore, we found that the gut microbiota was very distinct from that of the bedding and the feed.<h4>Significance</h4>The homogenous population response represents fundamental new knowledge about earthworm gut associated bacteria.
format	article
author	Knut Rudi Kristin Odegård Tine Therese Løkken Robert Wilson
author_facet	Knut Rudi Kristin Odegård Tine Therese Løkken Robert Wilson
author_sort	Knut Rudi
title	A feeding induced switch from a variable to a homogenous state of the earthworm gut microbiota within a host population.
title_short	A feeding induced switch from a variable to a homogenous state of the earthworm gut microbiota within a host population.
title_full	A feeding induced switch from a variable to a homogenous state of the earthworm gut microbiota within a host population.
title_fullStr	A feeding induced switch from a variable to a homogenous state of the earthworm gut microbiota within a host population.
title_full_unstemmed	A feeding induced switch from a variable to a homogenous state of the earthworm gut microbiota within a host population.
title_sort	feeding induced switch from a variable to a homogenous state of the earthworm gut microbiota within a host population.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/f5b696038d0245ab8fe9eb86fd34ca22
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A feeding induced switch from a variable to a homogenous state of the earthworm gut microbiota within a host population.

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