A Transient Receptor Potential-like Calcium Ion Channel in the Filamentous Fungus <i>Aspergillus nidulans</i>

Transient Receptor Potential (TRP) proteins constitute a superfamily that encodes transmembrane ion channels with highly diverse permeation and gating properties. Filamentous fungi possess putative TRP channel-encoded genes, but their functions remain elusive. Here, we report that a putative TRP-lik...

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Autores principales:	Hongchen Wang, Qiuyi Chen, Shizhu Zhang, Ling Lu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Transient Receptor Potential (TRP) calcium cell wall <i>Aspergillus nidulans</i> Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f5b7442a575d4bbc8173caad1efa25e1
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spelling	oai:doaj.org-article:f5b7442a575d4bbc8173caad1efa25e12021-11-25T18:05:45ZA Transient Receptor Potential-like Calcium Ion Channel in the Filamentous Fungus <i>Aspergillus nidulans</i>10.3390/jof71109202309-608Xhttps://doaj.org/article/f5b7442a575d4bbc8173caad1efa25e12021-10-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/920https://doaj.org/toc/2309-608XTransient Receptor Potential (TRP) proteins constitute a superfamily that encodes transmembrane ion channels with highly diverse permeation and gating properties. Filamentous fungi possess putative TRP channel-encoded genes, but their functions remain elusive. Here, we report that a putative TRP-like calcium channel, <i>trpR,</i> in the filamentous fungus <i>Aspergillus nidulans</i>, performs important roles in conidiation and in adapting to cell wall disruption reagents in a high temperature-induced defect-dependent manner, especially under a calcium-limited culture condition. The genetic and functional relationship between TrpR and the previously identified high-affinity calcium channels CchA/MidA indicates that TrpR has an opposite response to CchA/MidA when reacting to cell wall disruption reagents and in regulating calcium transients. However, a considerable addition of calcium can rescue all the defects that occur in TrpR and CchA/MidA, meaning that calcium is able to bypass the necessary requirement. Nevertheless, the colocalization at the membrane of the Golgi for TrpR and the P-type Golgi Ca<sup>2+</sup> ATPase PmrA suggests two channels that may work as ion transporters, transferring Ca<sup>2+</sup> from the cytosol into the Golgi apparatus and maintaining cellular calcium homeostasis. Therefore, combined with data for the <i>trpR</i> deletion mutant revealing abnormal cell wall structures, TrpR works as a Golgi membrane calcium ion channel that involves cell wall integration.Hongchen WangQiuyi ChenShizhu ZhangLing LuMDPI AGarticleTransient Receptor Potential (TRP)calciumcell wall<i>Aspergillus nidulans</i>Biology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 920, p 920 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Transient Receptor Potential (TRP) calcium cell wall <i>Aspergillus nidulans</i> Biology (General) QH301-705.5
spellingShingle	Transient Receptor Potential (TRP) calcium cell wall <i>Aspergillus nidulans</i> Biology (General) QH301-705.5 Hongchen Wang Qiuyi Chen Shizhu Zhang Ling Lu A Transient Receptor Potential-like Calcium Ion Channel in the Filamentous Fungus <i>Aspergillus nidulans</i>
description	Transient Receptor Potential (TRP) proteins constitute a superfamily that encodes transmembrane ion channels with highly diverse permeation and gating properties. Filamentous fungi possess putative TRP channel-encoded genes, but their functions remain elusive. Here, we report that a putative TRP-like calcium channel, <i>trpR,</i> in the filamentous fungus <i>Aspergillus nidulans</i>, performs important roles in conidiation and in adapting to cell wall disruption reagents in a high temperature-induced defect-dependent manner, especially under a calcium-limited culture condition. The genetic and functional relationship between TrpR and the previously identified high-affinity calcium channels CchA/MidA indicates that TrpR has an opposite response to CchA/MidA when reacting to cell wall disruption reagents and in regulating calcium transients. However, a considerable addition of calcium can rescue all the defects that occur in TrpR and CchA/MidA, meaning that calcium is able to bypass the necessary requirement. Nevertheless, the colocalization at the membrane of the Golgi for TrpR and the P-type Golgi Ca<sup>2+</sup> ATPase PmrA suggests two channels that may work as ion transporters, transferring Ca<sup>2+</sup> from the cytosol into the Golgi apparatus and maintaining cellular calcium homeostasis. Therefore, combined with data for the <i>trpR</i> deletion mutant revealing abnormal cell wall structures, TrpR works as a Golgi membrane calcium ion channel that involves cell wall integration.
format	article
author	Hongchen Wang Qiuyi Chen Shizhu Zhang Ling Lu
author_facet	Hongchen Wang Qiuyi Chen Shizhu Zhang Ling Lu
author_sort	Hongchen Wang
title	A Transient Receptor Potential-like Calcium Ion Channel in the Filamentous Fungus <i>Aspergillus nidulans</i>
title_short	A Transient Receptor Potential-like Calcium Ion Channel in the Filamentous Fungus <i>Aspergillus nidulans</i>
title_full	A Transient Receptor Potential-like Calcium Ion Channel in the Filamentous Fungus <i>Aspergillus nidulans</i>
title_fullStr	A Transient Receptor Potential-like Calcium Ion Channel in the Filamentous Fungus <i>Aspergillus nidulans</i>
title_full_unstemmed	A Transient Receptor Potential-like Calcium Ion Channel in the Filamentous Fungus <i>Aspergillus nidulans</i>
title_sort	transient receptor potential-like calcium ion channel in the filamentous fungus <i>aspergillus nidulans</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f5b7442a575d4bbc8173caad1efa25e1
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A Transient Receptor Potential-like Calcium Ion Channel in the Filamentous Fungus <i>Aspergillus nidulans</i>

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