ROLE OF DIFFERENT B CELL SUBPOPULATIONS IN IMMUNE RESPONSE TO T-INDEPENDENT TYPE 2 ANTIGENS

Abstract. The role of different B-cell subpopulations in polyclonal B-cell activation induced by T-independent antigens type 2 (TI-2) was under studies. CD5+B/B-1 cells were isolated from the spleens of mice immunized with polyvinyl pirrolidone, or á(1→3) dextran. The numbers of antibody (Ab) and Ig...

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Autores principales:	M. V. Gavrilova, I. N. Chernyshova, E. V. Sidorova
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	в-cells antibodies тн-2 antigens immunoglobulins Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/f5b8cb24864845d5bcb77eac08c52eb7
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spelling	oai:doaj.org-article:f5b8cb24864845d5bcb77eac08c52eb72021-11-18T08:03:38ZROLE OF DIFFERENT B CELL SUBPOPULATIONS IN IMMUNE RESPONSE TO T-INDEPENDENT TYPE 2 ANTIGENS1563-06252313-741X10.15789/1563-0625-2007-1-39-46https://doaj.org/article/f5b8cb24864845d5bcb77eac08c52eb72014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/118https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. The role of different B-cell subpopulations in polyclonal B-cell activation induced by T-independent antigens type 2 (TI-2) was under studies. CD5+B/B-1 cells were isolated from the spleens of mice immunized with polyvinyl pirrolidone, or á(1→3) dextran. The numbers of antibody (Ab) and Ig-producers in CD5+B/B-1 splenocytes were determined by ELISPOT. The number of cells producing unspecific Ig was calculated as a difference between the numbers of Ig- and Ab-producers; the numbers of nonspecific Ig-producing splenocytes induced by TI-2 immunization were determined as differences between their contents in immunized and control animals. In experiments with CD5+ and CD5– splenocytic populations, the development of Ab-producers and increased numbers of cells producing unspecific Ig was dependent on the CD5+ B-cells. These data were confirmed in experiments with subpopulations of B-1 and B-2 lymphocytes obtained with Dynal separation kit. In spite of sufficient predominance of B-cells in B-2 fraction (91% vs ~13% in B-1 fraction), the numbers of Ab and TI-2-induced nonspecific Ig-producers were nearly similar for the both cell fractions. Taking into account relative contents of B-cells, the numbers of Aband unspecific Ig- producers in B-1 fraction were 6- to 7-fold higher than in B-2 fraction. Thus, dependence on the B-1 cells exists both for polyclonal immune reactions to polyvinylpirrolidone and dextran, and specific response. Simultaneous injection of two TI-2 antigens did not induce additive effects upon the numbers of unspecific Ig-producers in B-1 fraction, in spite of marked increase in amounts of these cells after separate immunization with either of these antigens. It is concluded that polyclonal activation of B-1 cells by TI-2 antigens is subject to restriction which may depend either on the size of B-cell pool available for activation, or on insufficiency of appropriate stimulating factors.M. V. GavrilovaI. N. ChernyshovaE. V. SidorovaSPb RAACIarticleв-cellsantibodiesтн-2 antigensimmunoglobulinsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 9, Iss 1, Pp 39-46 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	в-cells antibodies тн-2 antigens immunoglobulins Immunologic diseases. Allergy RC581-607
spellingShingle	в-cells antibodies тн-2 antigens immunoglobulins Immunologic diseases. Allergy RC581-607 M. V. Gavrilova I. N. Chernyshova E. V. Sidorova ROLE OF DIFFERENT B CELL SUBPOPULATIONS IN IMMUNE RESPONSE TO T-INDEPENDENT TYPE 2 ANTIGENS
description	Abstract. The role of different B-cell subpopulations in polyclonal B-cell activation induced by T-independent antigens type 2 (TI-2) was under studies. CD5+B/B-1 cells were isolated from the spleens of mice immunized with polyvinyl pirrolidone, or á(1→3) dextran. The numbers of antibody (Ab) and Ig-producers in CD5+B/B-1 splenocytes were determined by ELISPOT. The number of cells producing unspecific Ig was calculated as a difference between the numbers of Ig- and Ab-producers; the numbers of nonspecific Ig-producing splenocytes induced by TI-2 immunization were determined as differences between their contents in immunized and control animals. In experiments with CD5+ and CD5– splenocytic populations, the development of Ab-producers and increased numbers of cells producing unspecific Ig was dependent on the CD5+ B-cells. These data were confirmed in experiments with subpopulations of B-1 and B-2 lymphocytes obtained with Dynal separation kit. In spite of sufficient predominance of B-cells in B-2 fraction (91% vs ~13% in B-1 fraction), the numbers of Ab and TI-2-induced nonspecific Ig-producers were nearly similar for the both cell fractions. Taking into account relative contents of B-cells, the numbers of Aband unspecific Ig- producers in B-1 fraction were 6- to 7-fold higher than in B-2 fraction. Thus, dependence on the B-1 cells exists both for polyclonal immune reactions to polyvinylpirrolidone and dextran, and specific response. Simultaneous injection of two TI-2 antigens did not induce additive effects upon the numbers of unspecific Ig-producers in B-1 fraction, in spite of marked increase in amounts of these cells after separate immunization with either of these antigens. It is concluded that polyclonal activation of B-1 cells by TI-2 antigens is subject to restriction which may depend either on the size of B-cell pool available for activation, or on insufficiency of appropriate stimulating factors.
format	article
author	M. V. Gavrilova I. N. Chernyshova E. V. Sidorova
author_facet	M. V. Gavrilova I. N. Chernyshova E. V. Sidorova
author_sort	M. V. Gavrilova
title	ROLE OF DIFFERENT B CELL SUBPOPULATIONS IN IMMUNE RESPONSE TO T-INDEPENDENT TYPE 2 ANTIGENS
title_short	ROLE OF DIFFERENT B CELL SUBPOPULATIONS IN IMMUNE RESPONSE TO T-INDEPENDENT TYPE 2 ANTIGENS
title_full	ROLE OF DIFFERENT B CELL SUBPOPULATIONS IN IMMUNE RESPONSE TO T-INDEPENDENT TYPE 2 ANTIGENS
title_fullStr	ROLE OF DIFFERENT B CELL SUBPOPULATIONS IN IMMUNE RESPONSE TO T-INDEPENDENT TYPE 2 ANTIGENS
title_full_unstemmed	ROLE OF DIFFERENT B CELL SUBPOPULATIONS IN IMMUNE RESPONSE TO T-INDEPENDENT TYPE 2 ANTIGENS
title_sort	role of different b cell subpopulations in immune response to t-independent type 2 antigens
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/f5b8cb24864845d5bcb77eac08c52eb7
work_keys_str_mv	AT mvgavrilova roleofdifferentbcellsubpopulationsinimmuneresponsetotindependenttype2antigens AT inchernyshova roleofdifferentbcellsubpopulationsinimmuneresponsetotindependenttype2antigens AT evsidorova roleofdifferentbcellsubpopulationsinimmuneresponsetotindependenttype2antigens
_version_	1718422580426178560

ROLE OF DIFFERENT B CELL SUBPOPULATIONS IN IMMUNE RESPONSE TO T-INDEPENDENT TYPE 2 ANTIGENS

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