Extensive Proliferation of Cd4+ Lymphocyte by Both Phytohaemagglutinin A and Anti-Cd2/Cd3/ Cd28 Macsibeads
Background: Lymphocytes proliferate considerably following appropriate stimulation in vitro. Autologous T cells are obtained from whole blood or tissue sites in relatively limited amounts. We need a method to expand these cells efficiently, study their functions and manipulate them to create appropr...
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oai:doaj.org-article:f5bfca0cea5240cba62d1f265bdac7e72021-12-02T17:31:31ZExtensive Proliferation of Cd4+ Lymphocyte by Both Phytohaemagglutinin A and Anti-Cd2/Cd3/ Cd28 Macsibeads0324-175010.2478/amb-2018-0004https://doaj.org/article/f5bfca0cea5240cba62d1f265bdac7e72018-03-01T00:00:00Zhttps://doi.org/10.2478/amb-2018-0004https://doaj.org/toc/0324-1750Background: Lymphocytes proliferate considerably following appropriate stimulation in vitro. Autologous T cells are obtained from whole blood or tissue sites in relatively limited amounts. We need a method to expand these cells efficiently, study their functions and manipulate them to create appropriate cells for transferring to the patient with infection and cancer. Objectives: The aim of this study is to determine proliferation ability of two different stimulators on CD4+ lymphocytes. Methods: Lymphocytes were isolated from blood samples of healthy donors after removing adherent cells (monocytes).The efficacy of MACSiBead™ coated with anti-CD2, anti-CD3, anti-CD28 (anti-CD2/CD3/CD28) was compared with Phytohaemagglutinin A (PHA) on CD4+ lymphocytes proliferation using carboxyfluorescein diacetate succinimidyl ester (CFSE) in cell culture media. The percentage of proliferating cells was analyzed using flow cytometry. Results: Both stimulators induced extensive proliferation of CD4+ lymphocytes but proliferation ability of PHA was higher compared to stimulation by anti-CD2/CD3/CD28 MACSiBead™. The proliferation rate of cells stimulated by PHA was 93.8% ± 3.37% whereas it was 85.2% ± 4.7% in cells stimulated by anti-CD2/CD3/CD28 MACSiBead™. Conclusions: Our results show that MACSiBead™ along with PHA can be used to obtain a large number of expanded CD4+ lymphocytes.Mirzakhani M.Shahbazi M.Darvish S.Mohammadnia-Afrouzi MousaSciendoarticleаnti-cd2/cd3/cd28 macsibead™phaproliferationMedicineRENActa Medica Bulgarica, Vol 45, Iss 1, Pp 22-25 (2018) |
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аnti-cd2/cd3/cd28 macsibead™ pha proliferation Medicine R |
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аnti-cd2/cd3/cd28 macsibead™ pha proliferation Medicine R Mirzakhani M. Shahbazi M. Darvish S. Mohammadnia-Afrouzi Mousa Extensive Proliferation of Cd4+ Lymphocyte by Both Phytohaemagglutinin A and Anti-Cd2/Cd3/ Cd28 Macsibeads |
| description |
Background: Lymphocytes proliferate considerably following appropriate stimulation in vitro. Autologous T cells are obtained from whole blood or tissue sites in relatively limited amounts. We need a method to expand these cells efficiently, study their functions and manipulate them to create appropriate cells for transferring to the patient with infection and cancer. Objectives: The aim of this study is to determine proliferation ability of two different stimulators on CD4+ lymphocytes. Methods: Lymphocytes were isolated from blood samples of healthy donors after removing adherent cells (monocytes).The efficacy of MACSiBead™ coated with anti-CD2, anti-CD3, anti-CD28 (anti-CD2/CD3/CD28) was compared with Phytohaemagglutinin A (PHA) on CD4+ lymphocytes proliferation using carboxyfluorescein diacetate succinimidyl ester (CFSE) in cell culture media. The percentage of proliferating cells was analyzed using flow cytometry. Results: Both stimulators induced extensive proliferation of CD4+ lymphocytes but proliferation ability of PHA was higher compared to stimulation by anti-CD2/CD3/CD28 MACSiBead™. The proliferation rate of cells stimulated by PHA was 93.8% ± 3.37% whereas it was 85.2% ± 4.7% in cells stimulated by anti-CD2/CD3/CD28 MACSiBead™. Conclusions: Our results show that MACSiBead™ along with PHA can be used to obtain a large number of expanded CD4+ lymphocytes. |
| format |
article |
| author |
Mirzakhani M. Shahbazi M. Darvish S. Mohammadnia-Afrouzi Mousa |
| author_facet |
Mirzakhani M. Shahbazi M. Darvish S. Mohammadnia-Afrouzi Mousa |
| author_sort |
Mirzakhani M. |
| title |
Extensive Proliferation of Cd4+ Lymphocyte by Both Phytohaemagglutinin A and Anti-Cd2/Cd3/ Cd28 Macsibeads |
| title_short |
Extensive Proliferation of Cd4+ Lymphocyte by Both Phytohaemagglutinin A and Anti-Cd2/Cd3/ Cd28 Macsibeads |
| title_full |
Extensive Proliferation of Cd4+ Lymphocyte by Both Phytohaemagglutinin A and Anti-Cd2/Cd3/ Cd28 Macsibeads |
| title_fullStr |
Extensive Proliferation of Cd4+ Lymphocyte by Both Phytohaemagglutinin A and Anti-Cd2/Cd3/ Cd28 Macsibeads |
| title_full_unstemmed |
Extensive Proliferation of Cd4+ Lymphocyte by Both Phytohaemagglutinin A and Anti-Cd2/Cd3/ Cd28 Macsibeads |
| title_sort |
extensive proliferation of cd4+ lymphocyte by both phytohaemagglutinin a and anti-cd2/cd3/ cd28 macsibeads |
| publisher |
Sciendo |
| publishDate |
2018 |
| url |
https://doaj.org/article/f5bfca0cea5240cba62d1f265bdac7e7 |
| work_keys_str_mv |
AT mirzakhanim extensiveproliferationofcd4lymphocytebybothphytohaemagglutininaandanticd2cd3cd28macsibeads AT shahbazim extensiveproliferationofcd4lymphocytebybothphytohaemagglutininaandanticd2cd3cd28macsibeads AT darvishs extensiveproliferationofcd4lymphocytebybothphytohaemagglutininaandanticd2cd3cd28macsibeads AT mohammadniaafrouzimousa extensiveproliferationofcd4lymphocytebybothphytohaemagglutininaandanticd2cd3cd28macsibeads |
| _version_ |
1718380589679116288 |