Oxidation–reduction potential and sperm DNA fragmentation, and their associations with sperm morphological anomalies amongst fertile and infertile men

Objective: To assess seminal oxidation–reduction potential (ORP) and sperm DNA fragmentation (SDF) in male infertility and their relationships with sperm morphology in fertile and infertile men. Patients and methods: Prospective case-control study comparing the findings of infertile men (n = 1168) t...

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Autores principales:	Ahmad Majzoub, Mohamad Arafa, Mohamed Mahdi, Ashok Agarwal, Sami Al Said, Ibrahim Al-Emadi, Walid El Ansari, Alia Alattar, Khalid Al Rumaihi, Haitham Elbardisi
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	Diseases of the genitourinary system. Urology RC870-923
Acceso en línea:	https://doaj.org/article/f5c04febecb046e6ac3bcb1fa1405a0f
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spelling	oai:doaj.org-article:f5c04febecb046e6ac3bcb1fa1405a0f2021-12-02T12:07:16ZOxidation–reduction potential and sperm DNA fragmentation, and their associations with sperm morphological anomalies amongst fertile and infertile men2090-598X10.1016/j.aju.2017.11.014https://doaj.org/article/f5c04febecb046e6ac3bcb1fa1405a0f2018-03-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2090598X17301481https://doaj.org/toc/2090-598XObjective: To assess seminal oxidation–reduction potential (ORP) and sperm DNA fragmentation (SDF) in male infertility and their relationships with sperm morphology in fertile and infertile men. Patients and methods: Prospective case-control study comparing the findings of infertile men (n = 1168) to those of men with confirmed fertility (n = 100) regarding demographics and semen characteristics (conventional and advanced semen tests). Spearman rank correlation assessed the correlation between ORP, SDF, and different morphological indices. Means of ORP and SDF were assessed in variable levels of normal sperm morphology amongst all participants. Results: Infertile patients had a significantly lower mean sperm count (32.7 vs 58.7 × 106 sperm/mL), total motility (50.1% vs 60.4%), and normal morphology (5.7% vs 9.9%). Conversely, infertile patients had significantly higher mean head defects (54% vs 48%), and higher ORP and SDF values than fertile controls. ORP and SDF showed significant positive correlations and significant negative correlations with sperm head defects and normal morphology in infertile patients, respectively. ORP and SDF were significantly inversely associated with the level of normal sperm morphology. Using receiver operating characteristic curve analysis, ORP and SDF threshold values of 1.73 mV/106 sperm/mL and 25.5%, respectively, were associated with 76% and 56% sensitivity and 72% and 72.2% specificity, respectively, in differentiating <4% from ≥4% normal morphology. Conclusion: A direct inverse relationship exists between seminal ORP and SDF with various levels of normal sperm morphology. Using ORP and SDF measures in conjunction with standard semen morphology analysis could validate the result of the fertility status of patients. Keywords: Male infertility, Oxidation-reduction potential, Sperm, Sperm DNA fragmentation, Sperm morphologyAhmad MajzoubMohamad ArafaMohamed MahdiAshok AgarwalSami Al SaidIbrahim Al-EmadiWalid El AnsariAlia AlattarKhalid Al RumaihiHaitham ElbardisiTaylor & Francis GrouparticleDiseases of the genitourinary system. UrologyRC870-923ENArab Journal of Urology, Vol 16, Iss 1, Pp 87-95 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Diseases of the genitourinary system. Urology RC870-923
spellingShingle	Diseases of the genitourinary system. Urology RC870-923 Ahmad Majzoub Mohamad Arafa Mohamed Mahdi Ashok Agarwal Sami Al Said Ibrahim Al-Emadi Walid El Ansari Alia Alattar Khalid Al Rumaihi Haitham Elbardisi Oxidation–reduction potential and sperm DNA fragmentation, and their associations with sperm morphological anomalies amongst fertile and infertile men
description	Objective: To assess seminal oxidation–reduction potential (ORP) and sperm DNA fragmentation (SDF) in male infertility and their relationships with sperm morphology in fertile and infertile men. Patients and methods: Prospective case-control study comparing the findings of infertile men (n = 1168) to those of men with confirmed fertility (n = 100) regarding demographics and semen characteristics (conventional and advanced semen tests). Spearman rank correlation assessed the correlation between ORP, SDF, and different morphological indices. Means of ORP and SDF were assessed in variable levels of normal sperm morphology amongst all participants. Results: Infertile patients had a significantly lower mean sperm count (32.7 vs 58.7 × 106 sperm/mL), total motility (50.1% vs 60.4%), and normal morphology (5.7% vs 9.9%). Conversely, infertile patients had significantly higher mean head defects (54% vs 48%), and higher ORP and SDF values than fertile controls. ORP and SDF showed significant positive correlations and significant negative correlations with sperm head defects and normal morphology in infertile patients, respectively. ORP and SDF were significantly inversely associated with the level of normal sperm morphology. Using receiver operating characteristic curve analysis, ORP and SDF threshold values of 1.73 mV/106 sperm/mL and 25.5%, respectively, were associated with 76% and 56% sensitivity and 72% and 72.2% specificity, respectively, in differentiating <4% from ≥4% normal morphology. Conclusion: A direct inverse relationship exists between seminal ORP and SDF with various levels of normal sperm morphology. Using ORP and SDF measures in conjunction with standard semen morphology analysis could validate the result of the fertility status of patients. Keywords: Male infertility, Oxidation-reduction potential, Sperm, Sperm DNA fragmentation, Sperm morphology
format	article
author	Ahmad Majzoub Mohamad Arafa Mohamed Mahdi Ashok Agarwal Sami Al Said Ibrahim Al-Emadi Walid El Ansari Alia Alattar Khalid Al Rumaihi Haitham Elbardisi
author_facet	Ahmad Majzoub Mohamad Arafa Mohamed Mahdi Ashok Agarwal Sami Al Said Ibrahim Al-Emadi Walid El Ansari Alia Alattar Khalid Al Rumaihi Haitham Elbardisi
author_sort	Ahmad Majzoub
title	Oxidation–reduction potential and sperm DNA fragmentation, and their associations with sperm morphological anomalies amongst fertile and infertile men
title_short	Oxidation–reduction potential and sperm DNA fragmentation, and their associations with sperm morphological anomalies amongst fertile and infertile men
title_full	Oxidation–reduction potential and sperm DNA fragmentation, and their associations with sperm morphological anomalies amongst fertile and infertile men
title_fullStr	Oxidation–reduction potential and sperm DNA fragmentation, and their associations with sperm morphological anomalies amongst fertile and infertile men
title_full_unstemmed	Oxidation–reduction potential and sperm DNA fragmentation, and their associations with sperm morphological anomalies amongst fertile and infertile men
title_sort	oxidation–reduction potential and sperm dna fragmentation, and their associations with sperm morphological anomalies amongst fertile and infertile men
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/f5c04febecb046e6ac3bcb1fa1405a0f
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Oxidation–reduction potential and sperm DNA fragmentation, and their associations with sperm morphological anomalies amongst fertile and infertile men

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