Rat embryonic mast cells originate in the AGM.
Mast cells originate from pluripotent hematopoietic stem cells. Two mast cell specific antibodies, mAbsAA4 and BGD6, have previously been used to identify and study committed mast cell precursors (MCcps) in the bone marrow of adult mice and rats. However, the embryonic origin of MCcps is still not k...
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2013
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oai:doaj.org-article:f5c30d5a156749b5a11c6c5734d7530c2021-11-18T07:54:26ZRat embryonic mast cells originate in the AGM.1932-620310.1371/journal.pone.0057862https://doaj.org/article/f5c30d5a156749b5a11c6c5734d7530c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23505443/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Mast cells originate from pluripotent hematopoietic stem cells. Two mast cell specific antibodies, mAbsAA4 and BGD6, have previously been used to identify and study committed mast cell precursors (MCcps) in the bone marrow of adult mice and rats. However, the embryonic origin of MCcps is still not known. In the present study, we identified MCcps in rat embryos using these previously characterized mast cell specific antibodies. The MCcps were found in the AGM (aorta-gonad-mesonephros) region of rat embryos at E11.5. These cells were BGD6+, CD34(+), c-kit(+), CD13(+), FcεRI(-), AA4(-) CD40(-), and Thy-1(-). By PCR the cells contained message for the α and β subunits of FcεRI and mast cell specific proteases. In vitro, the MCcps differentiated into metachromatic mast cells. With age of gestation the percent of MCcps diminished while the percent of mast cell progenitors increased. An increased knowledge of the biology and embryonic origin of mast cells may contribute to a greater understanding of allergy, asthma, and other mast cell related diseases.Michel Farchi GuiraldelliCarolina Nunes FrançaDevandir Antonio de SouzaElaine Zayas Marcelino da SilvaVanina Danuza TosoCeliane Cardoso CarvalhoMaria Célia JamurConstance OliverPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e57862 (2013) |
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DOAJ |
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DOAJ |
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Medicine R Science Q |
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Medicine R Science Q Michel Farchi Guiraldelli Carolina Nunes França Devandir Antonio de Souza Elaine Zayas Marcelino da Silva Vanina Danuza Toso Celiane Cardoso Carvalho Maria Célia Jamur Constance Oliver Rat embryonic mast cells originate in the AGM. |
| description |
Mast cells originate from pluripotent hematopoietic stem cells. Two mast cell specific antibodies, mAbsAA4 and BGD6, have previously been used to identify and study committed mast cell precursors (MCcps) in the bone marrow of adult mice and rats. However, the embryonic origin of MCcps is still not known. In the present study, we identified MCcps in rat embryos using these previously characterized mast cell specific antibodies. The MCcps were found in the AGM (aorta-gonad-mesonephros) region of rat embryos at E11.5. These cells were BGD6+, CD34(+), c-kit(+), CD13(+), FcεRI(-), AA4(-) CD40(-), and Thy-1(-). By PCR the cells contained message for the α and β subunits of FcεRI and mast cell specific proteases. In vitro, the MCcps differentiated into metachromatic mast cells. With age of gestation the percent of MCcps diminished while the percent of mast cell progenitors increased. An increased knowledge of the biology and embryonic origin of mast cells may contribute to a greater understanding of allergy, asthma, and other mast cell related diseases. |
| format |
article |
| author |
Michel Farchi Guiraldelli Carolina Nunes França Devandir Antonio de Souza Elaine Zayas Marcelino da Silva Vanina Danuza Toso Celiane Cardoso Carvalho Maria Célia Jamur Constance Oliver |
| author_facet |
Michel Farchi Guiraldelli Carolina Nunes França Devandir Antonio de Souza Elaine Zayas Marcelino da Silva Vanina Danuza Toso Celiane Cardoso Carvalho Maria Célia Jamur Constance Oliver |
| author_sort |
Michel Farchi Guiraldelli |
| title |
Rat embryonic mast cells originate in the AGM. |
| title_short |
Rat embryonic mast cells originate in the AGM. |
| title_full |
Rat embryonic mast cells originate in the AGM. |
| title_fullStr |
Rat embryonic mast cells originate in the AGM. |
| title_full_unstemmed |
Rat embryonic mast cells originate in the AGM. |
| title_sort |
rat embryonic mast cells originate in the agm. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/f5c30d5a156749b5a11c6c5734d7530c |
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