Insights in genetic diversity of German and Italian grape berry moth (Eupoecilia ambiguella) populations using novel microsatellite markers

Abstract The grape berry moth Eupoecilia ambiguella (Lepidoptera: Tortricidae) is causing significant damage to grape berries, however, little is known on population genetics of this lepidopteran pest insect, hindered so far by the lack of suitable molecular markers. Here we report on the developmen...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Annette Reineke, Alberto Pozzebon, Olivia Herczynski, Carlo Duso
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f5c713760e9d4f51af01493cac1f2837
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f5c713760e9d4f51af01493cac1f2837
record_format	dspace
spelling	oai:doaj.org-article:f5c713760e9d4f51af01493cac1f28372021-12-02T11:35:41ZInsights in genetic diversity of German and Italian grape berry moth (Eupoecilia ambiguella) populations using novel microsatellite markers10.1038/s41598-021-83855-02045-2322https://doaj.org/article/f5c713760e9d4f51af01493cac1f28372021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83855-0https://doaj.org/toc/2045-2322Abstract The grape berry moth Eupoecilia ambiguella (Lepidoptera: Tortricidae) is causing significant damage to grape berries, however, little is known on population genetics of this lepidopteran pest insect, hindered so far by the lack of suitable molecular markers. Here we report on the development of ten microsatellite markers of which six were used to characterise 21 E. ambiguella populations obtained from two viticultural regions in Germany and Italy. Moths were sampled during two subsequent generations (flights) in the same vineyard as well as in vineyards surrounded by different landscape types. German and Italian populations were genetically differentiated and a significant isolation by distance was evident. No significant divergence was observed among the populations from first or second flight moths, however, inbreeding was higher in first than in second flight populations. Moreover, inbreeding was influenced by habitat composition and complexity of landscape around vineyards, being positively associated with the percentage of area covered by grapevine. Population genetics of E. ambiguella could thus be affected by the presence of alternative host plants in viticultural landscapes, which is important in the light of both insecticide resistance management and sustainable pest management.Annette ReinekeAlberto PozzebonOlivia HerczynskiCarlo DusoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Annette Reineke Alberto Pozzebon Olivia Herczynski Carlo Duso Insights in genetic diversity of German and Italian grape berry moth (Eupoecilia ambiguella) populations using novel microsatellite markers
description	Abstract The grape berry moth Eupoecilia ambiguella (Lepidoptera: Tortricidae) is causing significant damage to grape berries, however, little is known on population genetics of this lepidopteran pest insect, hindered so far by the lack of suitable molecular markers. Here we report on the development of ten microsatellite markers of which six were used to characterise 21 E. ambiguella populations obtained from two viticultural regions in Germany and Italy. Moths were sampled during two subsequent generations (flights) in the same vineyard as well as in vineyards surrounded by different landscape types. German and Italian populations were genetically differentiated and a significant isolation by distance was evident. No significant divergence was observed among the populations from first or second flight moths, however, inbreeding was higher in first than in second flight populations. Moreover, inbreeding was influenced by habitat composition and complexity of landscape around vineyards, being positively associated with the percentage of area covered by grapevine. Population genetics of E. ambiguella could thus be affected by the presence of alternative host plants in viticultural landscapes, which is important in the light of both insecticide resistance management and sustainable pest management.
format	article
author	Annette Reineke Alberto Pozzebon Olivia Herczynski Carlo Duso
author_facet	Annette Reineke Alberto Pozzebon Olivia Herczynski Carlo Duso
author_sort	Annette Reineke
title	Insights in genetic diversity of German and Italian grape berry moth (Eupoecilia ambiguella) populations using novel microsatellite markers
title_short	Insights in genetic diversity of German and Italian grape berry moth (Eupoecilia ambiguella) populations using novel microsatellite markers
title_full	Insights in genetic diversity of German and Italian grape berry moth (Eupoecilia ambiguella) populations using novel microsatellite markers
title_fullStr	Insights in genetic diversity of German and Italian grape berry moth (Eupoecilia ambiguella) populations using novel microsatellite markers
title_full_unstemmed	Insights in genetic diversity of German and Italian grape berry moth (Eupoecilia ambiguella) populations using novel microsatellite markers
title_sort	insights in genetic diversity of german and italian grape berry moth (eupoecilia ambiguella) populations using novel microsatellite markers
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f5c713760e9d4f51af01493cac1f2837
work_keys_str_mv	AT annettereineke insightsingeneticdiversityofgermananditaliangrapeberrymotheupoeciliaambiguellapopulationsusingnovelmicrosatellitemarkers AT albertopozzebon insightsingeneticdiversityofgermananditaliangrapeberrymotheupoeciliaambiguellapopulationsusingnovelmicrosatellitemarkers AT oliviaherczynski insightsingeneticdiversityofgermananditaliangrapeberrymotheupoeciliaambiguellapopulationsusingnovelmicrosatellitemarkers AT carloduso insightsingeneticdiversityofgermananditaliangrapeberrymotheupoeciliaambiguellapopulationsusingnovelmicrosatellitemarkers
_version_	1718395856827187200

Insights in genetic diversity of German and Italian grape berry moth (Eupoecilia ambiguella) populations using novel microsatellite markers

Ejemplares similares