3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment

Physical signals provided by the extracellular matrix (ECM) are key microenvironmental parameters for the fate decision of hematopoietic stem and progenitor cells (HSPC) in bone marrow. Insights into cell-ECM interactions are critical for advancing HSC-based tissue engineering. Herein, we employed c...

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Autores principales:	Pan Zhang, Linmu Xu, Jingsong Gao, Guangkui Xu, Yanping Song, Guang Li, Jingjing Ren, Yunjie Zhang, Cheng Yang, Yu Zhang, Ruiheng Xie, Nu Zhang, Hui Yang
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Hematopoietic progenitor cells Collagen Matrix-dimensionality Macrophages Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f5c8d6db82a34a58bf2f58abfab572c3
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spelling	oai:doaj.org-article:f5c8d6db82a34a58bf2f58abfab572c32021-11-28T04:35:22Z3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment2452-199X10.1016/j.bioactmat.2021.08.032https://doaj.org/article/f5c8d6db82a34a58bf2f58abfab572c32022-04-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X21004084https://doaj.org/toc/2452-199XPhysical signals provided by the extracellular matrix (ECM) are key microenvironmental parameters for the fate decision of hematopoietic stem and progenitor cells (HSPC) in bone marrow. Insights into cell-ECM interactions are critical for advancing HSC-based tissue engineering. Herein, we employed collagen hydrogels and collagen-alginate hydrogels of defined stiffness to study the behaviors of hematopoietic progenitor cells (HPCs). Three-dimensional (3D) collagen hydrogels with a stiffness of 45 Pa were found to promote HPC maintenance and colony formation of monocyte/macrophage progenitors. Using single-cell RNA sequencing (scRNA-seq), we also characterized the comprehensive transcriptional profiles of cells randomly selected from two-dimensional (2D) and 3D hydrogels. A distinct maturation trajectory from HPCs into macrophages within the 3D microenvironment was revealed by these results. 3D-derived macrophages expressed high levels of various cytokines and chemokines, such as Saa3, Cxcl2, Socs3 and Tnf. Furthermore, enhanced communication between 3D-macrophages and other hematopoietic clusters based on ligand-repair interactions was demonstrated through bioinformatic analyses. Our research underlines the regulatory role of matrix-dimensionality in HPC differentiation and therefore probably be applied to the generation of specialized macrophages.Pan ZhangLinmu XuJingsong GaoGuangkui XuYanping SongGuang LiJingjing RenYunjie ZhangCheng YangYu ZhangRuiheng XieNu ZhangHui YangKeAi Communications Co., Ltd.articleHematopoietic progenitor cellsCollagenMatrix-dimensionalityMacrophagesMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 10, Iss , Pp 255-268 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hematopoietic progenitor cells Collagen Matrix-dimensionality Macrophages Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	Hematopoietic progenitor cells Collagen Matrix-dimensionality Macrophages Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Pan Zhang Linmu Xu Jingsong Gao Guangkui Xu Yanping Song Guang Li Jingjing Ren Yunjie Zhang Cheng Yang Yu Zhang Ruiheng Xie Nu Zhang Hui Yang 3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment
description	Physical signals provided by the extracellular matrix (ECM) are key microenvironmental parameters for the fate decision of hematopoietic stem and progenitor cells (HSPC) in bone marrow. Insights into cell-ECM interactions are critical for advancing HSC-based tissue engineering. Herein, we employed collagen hydrogels and collagen-alginate hydrogels of defined stiffness to study the behaviors of hematopoietic progenitor cells (HPCs). Three-dimensional (3D) collagen hydrogels with a stiffness of 45 Pa were found to promote HPC maintenance and colony formation of monocyte/macrophage progenitors. Using single-cell RNA sequencing (scRNA-seq), we also characterized the comprehensive transcriptional profiles of cells randomly selected from two-dimensional (2D) and 3D hydrogels. A distinct maturation trajectory from HPCs into macrophages within the 3D microenvironment was revealed by these results. 3D-derived macrophages expressed high levels of various cytokines and chemokines, such as Saa3, Cxcl2, Socs3 and Tnf. Furthermore, enhanced communication between 3D-macrophages and other hematopoietic clusters based on ligand-repair interactions was demonstrated through bioinformatic analyses. Our research underlines the regulatory role of matrix-dimensionality in HPC differentiation and therefore probably be applied to the generation of specialized macrophages.
format	article
author	Pan Zhang Linmu Xu Jingsong Gao Guangkui Xu Yanping Song Guang Li Jingjing Ren Yunjie Zhang Cheng Yang Yu Zhang Ruiheng Xie Nu Zhang Hui Yang
author_facet	Pan Zhang Linmu Xu Jingsong Gao Guangkui Xu Yanping Song Guang Li Jingjing Ren Yunjie Zhang Cheng Yang Yu Zhang Ruiheng Xie Nu Zhang Hui Yang
author_sort	Pan Zhang
title	3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment
title_short	3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment
title_full	3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment
title_fullStr	3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment
title_full_unstemmed	3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment
title_sort	3d collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/f5c8d6db82a34a58bf2f58abfab572c3
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3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment

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