A <italic toggle="yes">Sclerotinia sclerotiorum</italic> Transcription Factor Involved in Sclerotial Development and Virulence on Pea

ABSTRACT Sclerotinia sclerotiorum is a plant-pathogenic ascomycete fungus and infects over 400 host plants, including pea (Pisum sativum L.). The fungus causes white mold on pea, and substantial yield loss is attributed to the disease. To improve white mold management, further understanding of S. sc...

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Autores principales:	Hyunkyu Sang, Hao-Xun Chang, Martin I. Chilvers
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Sclerotinia sclerotiorum gene silencing pea sclerotial development transcription factor virulence Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f5cef0d91add4b29a913aa0fe700c36b
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spelling	oai:doaj.org-article:f5cef0d91add4b29a913aa0fe700c36b2021-11-15T15:22:05ZA <italic toggle="yes">Sclerotinia sclerotiorum</italic> Transcription Factor Involved in Sclerotial Development and Virulence on Pea10.1128/mSphere.00615-182379-5042https://doaj.org/article/f5cef0d91add4b29a913aa0fe700c36b2019-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00615-18https://doaj.org/toc/2379-5042ABSTRACT Sclerotinia sclerotiorum is a plant-pathogenic ascomycete fungus and infects over 400 host plants, including pea (Pisum sativum L.). The fungus causes white mold on pea, and substantial yield loss is attributed to the disease. To improve white mold management, further understanding of S. sclerotiorum pathogenicity is crucial. In this study, 389 transcription factors (TFs) were mined from the complete genome sequence of S. sclerotiorum and their in planta expression patterns were determined in susceptible and partially resistant pea lines and compared to in vitro expression patterns on culture medium. One of the transcription factors was significantly induced in planta at 24 and 48 h postinfection compared to the expression in vitro. This putative C6 transcription factor of S. sclerotiorum (SsC6TF1) was knocked down using a gene-silencing approach to investigate its functions in vegetative growth and sclerotial development as well as its virulence and pathogenicity in pea. While the SsC6TF1 knockdown mutants had hyphal growth rates identical to those of the wild-type strain and were capable of infection, the knockdown mutants produced no sclerotia or significantly fewer and smaller sclerotia on the culture medium and exhibited reduced virulence on both pea lines. This study profiled genome-wide expression for S. sclerotiorum transcription factors in planta and in vitro and functionally characterized a novel transcription factor, SsC6TF1, which positively regulates sclerotial development and virulence on pea. The finding provides molecular insights into S. sclerotiorum biology and interaction with pea and other economically important crops. IMPORTANCE White mold, caused by Sclerotinia sclerotiorum, is a destructive disease on important legume species such as soybean, dry bean, and pea. This study investigated expression levels of transcription factors in S. sclerotiorum in planta (pea lines) and in vitro (culture medium). One transcription factor displaying high expression in planta was found to be involved in sclerotial development and virulence on pea. This report provides a new understanding regarding transcription factors of S. sclerotiorum in development and virulence.Hyunkyu SangHao-Xun ChangMartin I. ChilversAmerican Society for MicrobiologyarticleSclerotinia sclerotiorumgene silencingpeasclerotial developmenttranscription factorvirulenceMicrobiologyQR1-502ENmSphere, Vol 4, Iss 1 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sclerotinia sclerotiorum gene silencing pea sclerotial development transcription factor virulence Microbiology QR1-502
spellingShingle	Sclerotinia sclerotiorum gene silencing pea sclerotial development transcription factor virulence Microbiology QR1-502 Hyunkyu Sang Hao-Xun Chang Martin I. Chilvers A <italic toggle="yes">Sclerotinia sclerotiorum</italic> Transcription Factor Involved in Sclerotial Development and Virulence on Pea
description	ABSTRACT Sclerotinia sclerotiorum is a plant-pathogenic ascomycete fungus and infects over 400 host plants, including pea (Pisum sativum L.). The fungus causes white mold on pea, and substantial yield loss is attributed to the disease. To improve white mold management, further understanding of S. sclerotiorum pathogenicity is crucial. In this study, 389 transcription factors (TFs) were mined from the complete genome sequence of S. sclerotiorum and their in planta expression patterns were determined in susceptible and partially resistant pea lines and compared to in vitro expression patterns on culture medium. One of the transcription factors was significantly induced in planta at 24 and 48 h postinfection compared to the expression in vitro. This putative C6 transcription factor of S. sclerotiorum (SsC6TF1) was knocked down using a gene-silencing approach to investigate its functions in vegetative growth and sclerotial development as well as its virulence and pathogenicity in pea. While the SsC6TF1 knockdown mutants had hyphal growth rates identical to those of the wild-type strain and were capable of infection, the knockdown mutants produced no sclerotia or significantly fewer and smaller sclerotia on the culture medium and exhibited reduced virulence on both pea lines. This study profiled genome-wide expression for S. sclerotiorum transcription factors in planta and in vitro and functionally characterized a novel transcription factor, SsC6TF1, which positively regulates sclerotial development and virulence on pea. The finding provides molecular insights into S. sclerotiorum biology and interaction with pea and other economically important crops. IMPORTANCE White mold, caused by Sclerotinia sclerotiorum, is a destructive disease on important legume species such as soybean, dry bean, and pea. This study investigated expression levels of transcription factors in S. sclerotiorum in planta (pea lines) and in vitro (culture medium). One transcription factor displaying high expression in planta was found to be involved in sclerotial development and virulence on pea. This report provides a new understanding regarding transcription factors of S. sclerotiorum in development and virulence.
format	article
author	Hyunkyu Sang Hao-Xun Chang Martin I. Chilvers
author_facet	Hyunkyu Sang Hao-Xun Chang Martin I. Chilvers
author_sort	Hyunkyu Sang
title	A <italic toggle="yes">Sclerotinia sclerotiorum</italic> Transcription Factor Involved in Sclerotial Development and Virulence on Pea
title_short	A <italic toggle="yes">Sclerotinia sclerotiorum</italic> Transcription Factor Involved in Sclerotial Development and Virulence on Pea
title_full	A <italic toggle="yes">Sclerotinia sclerotiorum</italic> Transcription Factor Involved in Sclerotial Development and Virulence on Pea
title_fullStr	A <italic toggle="yes">Sclerotinia sclerotiorum</italic> Transcription Factor Involved in Sclerotial Development and Virulence on Pea
title_full_unstemmed	A <italic toggle="yes">Sclerotinia sclerotiorum</italic> Transcription Factor Involved in Sclerotial Development and Virulence on Pea
title_sort	<italic toggle="yes">sclerotinia sclerotiorum</italic> transcription factor involved in sclerotial development and virulence on pea
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/f5cef0d91add4b29a913aa0fe700c36b
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A <italic toggle="yes">Sclerotinia sclerotiorum</italic> Transcription Factor Involved in Sclerotial Development and Virulence on Pea

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