Overexpression of protochlorophyllide oxidoreductase C regulates oxidative stress in Arabidopsis.

Light absorbed by colored intermediates of chlorophyll biosynthesis is not utilized in photosynthesis; instead, it is transferred to molecular oxygen, generating singlet oxygen ((1)O(2)). As there is no enzymatic detoxification mechanism available in plants to destroy (1)O(2), its generation should...

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Autores principales:	Gopal K Pattanayak, Baishnab C Tripathy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f5d4d93d886140158ffee84e753b71ed
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spelling	oai:doaj.org-article:f5d4d93d886140158ffee84e753b71ed2021-11-18T07:35:57ZOverexpression of protochlorophyllide oxidoreductase C regulates oxidative stress in Arabidopsis.1932-620310.1371/journal.pone.0026532https://doaj.org/article/f5d4d93d886140158ffee84e753b71ed2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22031838/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Light absorbed by colored intermediates of chlorophyll biosynthesis is not utilized in photosynthesis; instead, it is transferred to molecular oxygen, generating singlet oxygen ((1)O(2)). As there is no enzymatic detoxification mechanism available in plants to destroy (1)O(2), its generation should be minimized. We manipulated the concentration of a major chlorophyll biosynthetic intermediate i.e., protochlorophyllide in Arabidopsis by overexpressing the light-inducible protochlorophyllide oxidoreductase C (PORC) that effectively phototransforms endogenous protochlorophyllide to chlorophyllide leading to minimal accumulation of the photosensitizer protochlorophyllide in light-grown plants. In PORC overexpressing (PORCx) plants exposed to high-light, the (1)O(2) generation and consequent malonedialdehyde production was minimal and the maximum quantum efficiency of photosystem II remained unaffected demonstrating that their photosynthetic apparatus and cellular organization were intact. Further, PORCx plants treated with 5-aminolevulinicacid when exposed to light, photo-converted over-accumulated protochlorophyllide to chlorophyllide, reduced the generation of (1)O(2) and malonedialdehyde production and reduced plasma membrane damage. So PORCx plants survived and bolted whereas, the 5-aminolevulinicacid-treated wild-type plants perished. Thus, overexpression of PORC could be biotechnologically exploited in crop plants for tolerance to (1)O(2)-induced oxidative stress, paving the use of 5-aminolevulinicacid as a selective commercial light-activated biodegradable herbicide. Reduced protochlorophyllide content in PORCx plants released the protochlorophyllide-mediated feed-back inhibition of 5-aminolevulinicacid biosynthesis that resulted in higher 5-aminolevulinicacid production. Increase of 5-aminolevulinicacid synthesis upregulated the gene and protein expression of several downstream chlorophyll biosynthetic enzymes elucidating a regulatory net work of expression of genes involved in 5-aminolevulinicacid and tetrapyrrole biosynthesis.Gopal K PattanayakBaishnab C TripathyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26532 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gopal K Pattanayak Baishnab C Tripathy Overexpression of protochlorophyllide oxidoreductase C regulates oxidative stress in Arabidopsis.
description	Light absorbed by colored intermediates of chlorophyll biosynthesis is not utilized in photosynthesis; instead, it is transferred to molecular oxygen, generating singlet oxygen ((1)O(2)). As there is no enzymatic detoxification mechanism available in plants to destroy (1)O(2), its generation should be minimized. We manipulated the concentration of a major chlorophyll biosynthetic intermediate i.e., protochlorophyllide in Arabidopsis by overexpressing the light-inducible protochlorophyllide oxidoreductase C (PORC) that effectively phototransforms endogenous protochlorophyllide to chlorophyllide leading to minimal accumulation of the photosensitizer protochlorophyllide in light-grown plants. In PORC overexpressing (PORCx) plants exposed to high-light, the (1)O(2) generation and consequent malonedialdehyde production was minimal and the maximum quantum efficiency of photosystem II remained unaffected demonstrating that their photosynthetic apparatus and cellular organization were intact. Further, PORCx plants treated with 5-aminolevulinicacid when exposed to light, photo-converted over-accumulated protochlorophyllide to chlorophyllide, reduced the generation of (1)O(2) and malonedialdehyde production and reduced plasma membrane damage. So PORCx plants survived and bolted whereas, the 5-aminolevulinicacid-treated wild-type plants perished. Thus, overexpression of PORC could be biotechnologically exploited in crop plants for tolerance to (1)O(2)-induced oxidative stress, paving the use of 5-aminolevulinicacid as a selective commercial light-activated biodegradable herbicide. Reduced protochlorophyllide content in PORCx plants released the protochlorophyllide-mediated feed-back inhibition of 5-aminolevulinicacid biosynthesis that resulted in higher 5-aminolevulinicacid production. Increase of 5-aminolevulinicacid synthesis upregulated the gene and protein expression of several downstream chlorophyll biosynthetic enzymes elucidating a regulatory net work of expression of genes involved in 5-aminolevulinicacid and tetrapyrrole biosynthesis.
format	article
author	Gopal K Pattanayak Baishnab C Tripathy
author_facet	Gopal K Pattanayak Baishnab C Tripathy
author_sort	Gopal K Pattanayak
title	Overexpression of protochlorophyllide oxidoreductase C regulates oxidative stress in Arabidopsis.
title_short	Overexpression of protochlorophyllide oxidoreductase C regulates oxidative stress in Arabidopsis.
title_full	Overexpression of protochlorophyllide oxidoreductase C regulates oxidative stress in Arabidopsis.
title_fullStr	Overexpression of protochlorophyllide oxidoreductase C regulates oxidative stress in Arabidopsis.
title_full_unstemmed	Overexpression of protochlorophyllide oxidoreductase C regulates oxidative stress in Arabidopsis.
title_sort	overexpression of protochlorophyllide oxidoreductase c regulates oxidative stress in arabidopsis.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/f5d4d93d886140158ffee84e753b71ed
work_keys_str_mv	AT gopalkpattanayak overexpressionofprotochlorophyllideoxidoreductasecregulatesoxidativestressinarabidopsis AT baishnabctripathy overexpressionofprotochlorophyllideoxidoreductasecregulatesoxidativestressinarabidopsis
_version_	1718423261655597056

Overexpression of protochlorophyllide oxidoreductase C regulates oxidative stress in Arabidopsis.

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