Dynamic Expression and Regulatory Network of Circular RNA for Abdominal Preadipocytes Differentiation in Chicken (Gallus gallus)

Circular RNA (circRNA), as a novel endogenous biomolecule, has been emergingly demonstrated to play crucial roles in mammalian lipid metabolism and obesity. However, little is known about their genome-wide identification, expression profile, and function in chicken adipogenesis. In present study, th...

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Autores principales:	Weihua Tian, Bo Zhang, Haian Zhong, Ruixue Nie, Yao Ling, Hao Zhang, Changxin Wu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	chicken circRNA abdominal fat adipogenic differentiation competing endogenous RNA Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f5d961e986db4736924b96d644873a7f
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spelling	oai:doaj.org-article:f5d961e986db4736924b96d644873a7f2021-11-12T05:48:30ZDynamic Expression and Regulatory Network of Circular RNA for Abdominal Preadipocytes Differentiation in Chicken (Gallus gallus)2296-634X10.3389/fcell.2021.761638https://doaj.org/article/f5d961e986db4736924b96d644873a7f2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.761638/fullhttps://doaj.org/toc/2296-634XCircular RNA (circRNA), as a novel endogenous biomolecule, has been emergingly demonstrated to play crucial roles in mammalian lipid metabolism and obesity. However, little is known about their genome-wide identification, expression profile, and function in chicken adipogenesis. In present study, the adipogenic differentiation of chicken abdominal preadipocyte was successfully induced, and the regulatory functional circRNAs in chicken adipogenesis were identified from abdominal adipocytes at different differentiation stages using Ribo-Zero RNA-seq. A total of 1,068 circRNA candidates were identified and mostly derived from exons. Of these, 111 differentially expressed circRNAs (DE-circRNAs) were detected, characterized by stage-specific expression, and enriched in several lipid-related pathways, such as Hippo signaling pathway, mTOR signaling pathway. Through weighted gene co-expression network analyses (WGCNA) and K-means clustering analyses, two DE-circRNAs, Z:35565770\|35568133 and Z:54674624\|54755962, were identified as candidate regulatory circRNAs in chicken adipogenic differentiation. Z:35565770\|35568133 might compete splicing with its parental gene, ABHD17B, owing to its strictly negative co-expression. We also constructed competing endogenous RNA (ceRNA) network based on DE-circRNA, DE-miRNA, DE-mRNAs, revealing that Z:54674624\|54755962 might function as a ceRNA to regulate chicken adipogenic differentiation through the gga-miR-1635-AHR2/IRF1/MGAT3/ABCA1/AADAC and/or the novel_miR_232-STAT5A axis. Translation activity analysis showed that Z:35565770\|35568133 and Z:54674624\|54755962 have no protein-coding potential. These findings provide valuable evidence for a better understanding of the specific functions and molecular mechanisms of circRNAs underlying avian adipogenesis.Weihua TianWeihua TianBo ZhangBo ZhangHaian ZhongHaian ZhongRuixue NieRuixue NieYao LingYao LingHao ZhangHao ZhangChangxin WuChangxin WuFrontiers Media S.A.articlechickencircRNAabdominal fatadipogenic differentiationcompeting endogenous RNABiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chicken circRNA abdominal fat adipogenic differentiation competing endogenous RNA Biology (General) QH301-705.5
spellingShingle	chicken circRNA abdominal fat adipogenic differentiation competing endogenous RNA Biology (General) QH301-705.5 Weihua Tian Weihua Tian Bo Zhang Bo Zhang Haian Zhong Haian Zhong Ruixue Nie Ruixue Nie Yao Ling Yao Ling Hao Zhang Hao Zhang Changxin Wu Changxin Wu Dynamic Expression and Regulatory Network of Circular RNA for Abdominal Preadipocytes Differentiation in Chicken (Gallus gallus)
description	Circular RNA (circRNA), as a novel endogenous biomolecule, has been emergingly demonstrated to play crucial roles in mammalian lipid metabolism and obesity. However, little is known about their genome-wide identification, expression profile, and function in chicken adipogenesis. In present study, the adipogenic differentiation of chicken abdominal preadipocyte was successfully induced, and the regulatory functional circRNAs in chicken adipogenesis were identified from abdominal adipocytes at different differentiation stages using Ribo-Zero RNA-seq. A total of 1,068 circRNA candidates were identified and mostly derived from exons. Of these, 111 differentially expressed circRNAs (DE-circRNAs) were detected, characterized by stage-specific expression, and enriched in several lipid-related pathways, such as Hippo signaling pathway, mTOR signaling pathway. Through weighted gene co-expression network analyses (WGCNA) and K-means clustering analyses, two DE-circRNAs, Z:35565770\|35568133 and Z:54674624\|54755962, were identified as candidate regulatory circRNAs in chicken adipogenic differentiation. Z:35565770\|35568133 might compete splicing with its parental gene, ABHD17B, owing to its strictly negative co-expression. We also constructed competing endogenous RNA (ceRNA) network based on DE-circRNA, DE-miRNA, DE-mRNAs, revealing that Z:54674624\|54755962 might function as a ceRNA to regulate chicken adipogenic differentiation through the gga-miR-1635-AHR2/IRF1/MGAT3/ABCA1/AADAC and/or the novel_miR_232-STAT5A axis. Translation activity analysis showed that Z:35565770\|35568133 and Z:54674624\|54755962 have no protein-coding potential. These findings provide valuable evidence for a better understanding of the specific functions and molecular mechanisms of circRNAs underlying avian adipogenesis.
format	article
author	Weihua Tian Weihua Tian Bo Zhang Bo Zhang Haian Zhong Haian Zhong Ruixue Nie Ruixue Nie Yao Ling Yao Ling Hao Zhang Hao Zhang Changxin Wu Changxin Wu
author_facet	Weihua Tian Weihua Tian Bo Zhang Bo Zhang Haian Zhong Haian Zhong Ruixue Nie Ruixue Nie Yao Ling Yao Ling Hao Zhang Hao Zhang Changxin Wu Changxin Wu
author_sort	Weihua Tian
title	Dynamic Expression and Regulatory Network of Circular RNA for Abdominal Preadipocytes Differentiation in Chicken (Gallus gallus)
title_short	Dynamic Expression and Regulatory Network of Circular RNA for Abdominal Preadipocytes Differentiation in Chicken (Gallus gallus)
title_full	Dynamic Expression and Regulatory Network of Circular RNA for Abdominal Preadipocytes Differentiation in Chicken (Gallus gallus)
title_fullStr	Dynamic Expression and Regulatory Network of Circular RNA for Abdominal Preadipocytes Differentiation in Chicken (Gallus gallus)
title_full_unstemmed	Dynamic Expression and Regulatory Network of Circular RNA for Abdominal Preadipocytes Differentiation in Chicken (Gallus gallus)
title_sort	dynamic expression and regulatory network of circular rna for abdominal preadipocytes differentiation in chicken (gallus gallus)
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f5d961e986db4736924b96d644873a7f
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Dynamic Expression and Regulatory Network of Circular RNA for Abdominal Preadipocytes Differentiation in Chicken (Gallus gallus)

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