Dopamine receptors in human adipocytes: expression and functions.

<h4>Introduction</h4>Dopamine (DA) binds to five receptors (DAR), classified by their ability to increase (D1R-like) or decrease (D2R-like) cAMP. In humans, most DA circulates as dopamine sulfate (DA-S), which can be de-conjugated to bioactive DA by arylsulfatase A (ARSA). The objective...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dana C Borcherding, Eric R Hugo, Gila Idelman, Anuradha De Silva, Nathan W Richtand, Jean Loftus, Nira Ben-Jonathan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f5dba42807724d7fa478d4bcba590595
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f5dba42807724d7fa478d4bcba590595
record_format	dspace
spelling	oai:doaj.org-article:f5dba42807724d7fa478d4bcba5905952021-11-04T06:07:46ZDopamine receptors in human adipocytes: expression and functions.1932-620310.1371/journal.pone.0025537https://doaj.org/article/f5dba42807724d7fa478d4bcba5905952011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21966540/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Introduction</h4>Dopamine (DA) binds to five receptors (DAR), classified by their ability to increase (D1R-like) or decrease (D2R-like) cAMP. In humans, most DA circulates as dopamine sulfate (DA-S), which can be de-conjugated to bioactive DA by arylsulfatase A (ARSA). The objective was to examine expression of DAR and ARSA in human adipose tissue and determine whether DA regulates prolactin (PRL) and adipokine expression and release.<h4>Methods</h4>DAR were analyzed by RT-PCR and Western blotting in explants, primary adipocytes and two human adipocyte cell lines, LS14 and SW872. ARSA expression and activity were determined by qPCR and enzymatic assay. PRL expression and release were determined by luciferase reporter and Nb2 bioassay. Analysis of cAMP, cGMP, leptin, adiponectin and interleukin 6 (IL-6) was done by ELISA. Activation of MAPK and PI3 kinase/Akt was determined by Western blotting.<h4>Results</h4>DAR are variably expressed at the mRNA and protein levels in adipose tissue and adipocytes during adipogenesis. ARSA activity in adipocyte increases after differentiation. DA at nM concentrations suppresses cAMP, stimulates cGMP, and activates MAPK in adipocytes. Acting via D2R-like receptors, DA and DA-S inhibit PRL gene expression and release. Acting via D1R/D5R receptors, DA suppresses leptin and stimulates adiponectin and IL-6 release.<h4>Conclusions</h4>This is the first report that human adipocytes express functional DAR and ARSA, suggesting a regulatory role for peripheral DA in adipose functions. We speculate that the propensity of some DAR-activating antipsychotics to increase weight and alter metabolic homeostasis is due, in part, to their direct action on adipose tissue.Dana C BorcherdingEric R HugoGila IdelmanAnuradha De SilvaNathan W RichtandJean LoftusNira Ben-JonathanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e25537 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dana C Borcherding Eric R Hugo Gila Idelman Anuradha De Silva Nathan W Richtand Jean Loftus Nira Ben-Jonathan Dopamine receptors in human adipocytes: expression and functions.
description	<h4>Introduction</h4>Dopamine (DA) binds to five receptors (DAR), classified by their ability to increase (D1R-like) or decrease (D2R-like) cAMP. In humans, most DA circulates as dopamine sulfate (DA-S), which can be de-conjugated to bioactive DA by arylsulfatase A (ARSA). The objective was to examine expression of DAR and ARSA in human adipose tissue and determine whether DA regulates prolactin (PRL) and adipokine expression and release.<h4>Methods</h4>DAR were analyzed by RT-PCR and Western blotting in explants, primary adipocytes and two human adipocyte cell lines, LS14 and SW872. ARSA expression and activity were determined by qPCR and enzymatic assay. PRL expression and release were determined by luciferase reporter and Nb2 bioassay. Analysis of cAMP, cGMP, leptin, adiponectin and interleukin 6 (IL-6) was done by ELISA. Activation of MAPK and PI3 kinase/Akt was determined by Western blotting.<h4>Results</h4>DAR are variably expressed at the mRNA and protein levels in adipose tissue and adipocytes during adipogenesis. ARSA activity in adipocyte increases after differentiation. DA at nM concentrations suppresses cAMP, stimulates cGMP, and activates MAPK in adipocytes. Acting via D2R-like receptors, DA and DA-S inhibit PRL gene expression and release. Acting via D1R/D5R receptors, DA suppresses leptin and stimulates adiponectin and IL-6 release.<h4>Conclusions</h4>This is the first report that human adipocytes express functional DAR and ARSA, suggesting a regulatory role for peripheral DA in adipose functions. We speculate that the propensity of some DAR-activating antipsychotics to increase weight and alter metabolic homeostasis is due, in part, to their direct action on adipose tissue.
format	article
author	Dana C Borcherding Eric R Hugo Gila Idelman Anuradha De Silva Nathan W Richtand Jean Loftus Nira Ben-Jonathan
author_facet	Dana C Borcherding Eric R Hugo Gila Idelman Anuradha De Silva Nathan W Richtand Jean Loftus Nira Ben-Jonathan
author_sort	Dana C Borcherding
title	Dopamine receptors in human adipocytes: expression and functions.
title_short	Dopamine receptors in human adipocytes: expression and functions.
title_full	Dopamine receptors in human adipocytes: expression and functions.
title_fullStr	Dopamine receptors in human adipocytes: expression and functions.
title_full_unstemmed	Dopamine receptors in human adipocytes: expression and functions.
title_sort	dopamine receptors in human adipocytes: expression and functions.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/f5dba42807724d7fa478d4bcba590595
work_keys_str_mv	AT danacborcherding dopaminereceptorsinhumanadipocytesexpressionandfunctions AT ericrhugo dopaminereceptorsinhumanadipocytesexpressionandfunctions AT gilaidelman dopaminereceptorsinhumanadipocytesexpressionandfunctions AT anuradhadesilva dopaminereceptorsinhumanadipocytesexpressionandfunctions AT nathanwrichtand dopaminereceptorsinhumanadipocytesexpressionandfunctions AT jeanloftus dopaminereceptorsinhumanadipocytesexpressionandfunctions AT nirabenjonathan dopaminereceptorsinhumanadipocytesexpressionandfunctions
_version_	1718445187788701696

Dopamine receptors in human adipocytes: expression and functions.

Ejemplares similares