Phocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus
Yuxin Wang,1,* Meishuo Ouyang,2,* Xibao Gao,1 Shuai Wang,3 Chunyang Fu,1 Jiayi Zeng,1 Xiaodong He1,4 1Department of Physical and Chemical Inspection, School of Public Health, Shandong University, Jinan, Shandong 250012, People’s Republic of China; 2Department of Public Health, Graduate Sch...
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Dove Medical Press
2020
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oai:doaj.org-article:f5dc517e52ad4200adcd91b1e38b41d52021-12-02T11:51:20ZPhocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus1178-7007https://doaj.org/article/f5dc517e52ad4200adcd91b1e38b41d52020-03-01T00:00:00Zhttps://www.dovepress.com/phocea-pseudoflavonifractor-and-lactobacillus-intestinalis-three-poten-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Yuxin Wang,1,* Meishuo Ouyang,2,* Xibao Gao,1 Shuai Wang,3 Chunyang Fu,1 Jiayi Zeng,1 Xiaodong He1,4 1Department of Physical and Chemical Inspection, School of Public Health, Shandong University, Jinan, Shandong 250012, People’s Republic of China; 2Department of Public Health, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan; 3Institute of Toxicology, School of Public Health, Shandong University, Jinan, Shandong 250012, People’s Republic of China; 4Shandong Provincial Key Laboratory of Infection and Immunity, School of Basic Medical Sciences, Shandong University, Jinan, Shandong 250012, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiaodong HeDepartment of Physical and Chemical Inspection, School of Public Health, Shandong University, 44 West Wenhua Road, Jinan, Shandong 250012, People’s Republic of ChinaTel/Fax +86 531 88382554Email xiaodong.he@sdu.edu.cnPurpose: The purpose of this study was to explore the difference and association between intestinal microbiota and plasma metabolomics between type 2 diabetes mellitus (T2DM) and normal group and to identify potential microbiota biomarkers that contribute the most to the difference in metabolites.Methods: Six male ZDF model (fa/fa) rats were fed by a Purina #5008 Lab Diet (crude protein 23.5%, crude fat 6.5%) for 3 weeks and their age-matched 6 ZDF control (fa/+) rats were fed by normal rodent diet. Their stool and blood samples were collected at 12 weeks. To analyze the microbial populations in these samples, we used a 16S rRNA gene sequencing approach. Liquid chromatography–mass spectrometry (LC-MS) followed by multivariate statistical analysis was applied to the plasma metabolites profiling. Correlation analysis of them was calculated by Pearson statistical method.Results: Twelve potential biomarkers of intestinal microbial flora and 357 differential metabolites were found in ZDF fa/fa rats, among which there are three flora that contributed the most to the perturbation of metabolites, including genus Phocea, Pseudoflavonifractor and species Lactobacillus intestinalis.Conclusion: Our study demonstrates the alterations of the abundance and diversity of the intestinal microbiota and the perturbation of metabolites in ZDF rats (fa/fa). We found three potential biomarkers of intestinal microbiota that may lead to perturbation in plasma metabolites. This may prompt new pathogenesis of obesity-related T2DM, but we also need to study further about the causal relationship between intestinal microbe and T2DM, so as to find the target of T2DM treatment or preventive measures.Keywords: type 2 diabetes mellitus, Zucker diabetic fatty rats, 16S rRNA sequencing, intestinal microbiota, plasma metabolomicsWang YOuyang MGao XWang SFu CZeng JHe XDove Medical Pressarticletype 2 diabetes mellituszucker diabetic fatty rats16s rrna sequencingintestinal microbiotaplasma metabolomicsSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 835-850 (2020) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
type 2 diabetes mellitus zucker diabetic fatty rats 16s rrna sequencing intestinal microbiota plasma metabolomics Specialties of internal medicine RC581-951 |
| spellingShingle |
type 2 diabetes mellitus zucker diabetic fatty rats 16s rrna sequencing intestinal microbiota plasma metabolomics Specialties of internal medicine RC581-951 Wang Y Ouyang M Gao X Wang S Fu C Zeng J He X Phocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus |
| description |
Yuxin Wang,1,* Meishuo Ouyang,2,* Xibao Gao,1 Shuai Wang,3 Chunyang Fu,1 Jiayi Zeng,1 Xiaodong He1,4 1Department of Physical and Chemical Inspection, School of Public Health, Shandong University, Jinan, Shandong 250012, People’s Republic of China; 2Department of Public Health, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan; 3Institute of Toxicology, School of Public Health, Shandong University, Jinan, Shandong 250012, People’s Republic of China; 4Shandong Provincial Key Laboratory of Infection and Immunity, School of Basic Medical Sciences, Shandong University, Jinan, Shandong 250012, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiaodong HeDepartment of Physical and Chemical Inspection, School of Public Health, Shandong University, 44 West Wenhua Road, Jinan, Shandong 250012, People’s Republic of ChinaTel/Fax +86 531 88382554Email xiaodong.he@sdu.edu.cnPurpose: The purpose of this study was to explore the difference and association between intestinal microbiota and plasma metabolomics between type 2 diabetes mellitus (T2DM) and normal group and to identify potential microbiota biomarkers that contribute the most to the difference in metabolites.Methods: Six male ZDF model (fa/fa) rats were fed by a Purina #5008 Lab Diet (crude protein 23.5%, crude fat 6.5%) for 3 weeks and their age-matched 6 ZDF control (fa/+) rats were fed by normal rodent diet. Their stool and blood samples were collected at 12 weeks. To analyze the microbial populations in these samples, we used a 16S rRNA gene sequencing approach. Liquid chromatography–mass spectrometry (LC-MS) followed by multivariate statistical analysis was applied to the plasma metabolites profiling. Correlation analysis of them was calculated by Pearson statistical method.Results: Twelve potential biomarkers of intestinal microbial flora and 357 differential metabolites were found in ZDF fa/fa rats, among which there are three flora that contributed the most to the perturbation of metabolites, including genus Phocea, Pseudoflavonifractor and species Lactobacillus intestinalis.Conclusion: Our study demonstrates the alterations of the abundance and diversity of the intestinal microbiota and the perturbation of metabolites in ZDF rats (fa/fa). We found three potential biomarkers of intestinal microbiota that may lead to perturbation in plasma metabolites. This may prompt new pathogenesis of obesity-related T2DM, but we also need to study further about the causal relationship between intestinal microbe and T2DM, so as to find the target of T2DM treatment or preventive measures.Keywords: type 2 diabetes mellitus, Zucker diabetic fatty rats, 16S rRNA sequencing, intestinal microbiota, plasma metabolomics |
| format |
article |
| author |
Wang Y Ouyang M Gao X Wang S Fu C Zeng J He X |
| author_facet |
Wang Y Ouyang M Gao X Wang S Fu C Zeng J He X |
| author_sort |
Wang Y |
| title |
Phocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus |
| title_short |
Phocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus |
| title_full |
Phocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus |
| title_fullStr |
Phocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus |
| title_full_unstemmed |
Phocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus |
| title_sort |
phocea, pseudoflavonifractor and lactobacillus intestinalis: three potential biomarkers of gut microbiota that affect progression and complications of obesity-induced type 2 diabetes mellitus |
| publisher |
Dove Medical Press |
| publishDate |
2020 |
| url |
https://doaj.org/article/f5dc517e52ad4200adcd91b1e38b41d5 |
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