Citral modulates human monocyte responses to Staphylococcus aureus infection

Abstract Staphylococcus aureus is a Gram-positive bacterium that is considered an important human pathogen. Due to its virulence and ability to acquire mechanisms of resistance to antibiotics, the clinical severity of S. aureus infection is driven by inflammatory responses to the bacteria. Thus, the...

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Autores principales:	Hellen Braga Martins Oliveira, Nathan das Neves Selis, Thamara Louisy Santos Brito, Beatriz Almeida Sampaio, Rafaela de Souza Bittencourt, Caline Novais Teixeira Oliveira, Manoel Neres Santos Júnior, Carolline Florentino Almeida, Palloma Porto Almeida, Guilherme Barreto Campos, Aline Teixeira Amorim, Jorge Timenetsky, Carla Cristina Romano, Ana Paula Trovatti Uetanabaro, Regiane Yatsuda, Lucas Miranda Marques
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f5e3d110964d44c484a655bfbafd9696
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spelling	oai:doaj.org-article:f5e3d110964d44c484a655bfbafd96962021-11-14T12:18:02ZCitral modulates human monocyte responses to Staphylococcus aureus infection10.1038/s41598-021-01536-42045-2322https://doaj.org/article/f5e3d110964d44c484a655bfbafd96962021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01536-4https://doaj.org/toc/2045-2322Abstract Staphylococcus aureus is a Gram-positive bacterium that is considered an important human pathogen. Due to its virulence and ability to acquire mechanisms of resistance to antibiotics, the clinical severity of S. aureus infection is driven by inflammatory responses to the bacteria. Thus, the present study aimed to investigate the modulating role of citral in inflammation caused by S. aureus infection. For this, we used an isolate obtained from a nasal swab sample of a healthy child attending a day-care centre in Vitória da Conquista, Bahia, Brazil. The role of citral in modulating immunological factors against S. aureus infection was evaluated by isolating and cultivating human peripheral blood mononuclear cells. The monocytes were treated with 4%, 2%, and 1% citral before and after inoculation with S. aureus. The cells were analysed by immunophenotyping of monocyte cell surface molecules (CD54, CD282, CD80, HLA-DR, and CD86) and cytokine dosage (IL-1β, IL-6, IL-10, IL-12p70, IL-23, IFN-γ, TGF-β, and TNF-α), and evaluated for the expression of 84 genes related to innate and adaptive immune system responses. GraphPad Prism software and variables with P values < 0.05, were used for statistical analysis. Our data demonstrated citral’s action on the expression of surface markers involved in recognition, presentation, and migration, such as CD14, CD54, and CD80, in global negative regulation of inflammation with inhibitory effects on NF-κB, JNK/p38, and IFN pathways. Consequently, IL-1β, IL-6, IL-12p70, IL-23, IFN-γ, and TNF-α cytokine expression was reduced in groups treated with citral and groups treated with citral at 4%, 2%, and 1% and infected, and levels of anti-inflammatory cytokines such as IL-10 were increased. Furthermore, citral could be used as a supporting anti-inflammatory agent against infections caused by S. aureus. There are no data correlating citral, S. aureus, and the markers analysed here; thus, our study addresses this gap in the literature.Hellen Braga Martins OliveiraNathan das Neves SelisThamara Louisy Santos BritoBeatriz Almeida SampaioRafaela de Souza BittencourtCaline Novais Teixeira OliveiraManoel Neres Santos JúniorCarolline Florentino AlmeidaPalloma Porto AlmeidaGuilherme Barreto CamposAline Teixeira AmorimJorge TimenetskyCarla Cristina RomanoAna Paula Trovatti UetanabaroRegiane YatsudaLucas Miranda MarquesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hellen Braga Martins Oliveira Nathan das Neves Selis Thamara Louisy Santos Brito Beatriz Almeida Sampaio Rafaela de Souza Bittencourt Caline Novais Teixeira Oliveira Manoel Neres Santos Júnior Carolline Florentino Almeida Palloma Porto Almeida Guilherme Barreto Campos Aline Teixeira Amorim Jorge Timenetsky Carla Cristina Romano Ana Paula Trovatti Uetanabaro Regiane Yatsuda Lucas Miranda Marques Citral modulates human monocyte responses to Staphylococcus aureus infection
description	Abstract Staphylococcus aureus is a Gram-positive bacterium that is considered an important human pathogen. Due to its virulence and ability to acquire mechanisms of resistance to antibiotics, the clinical severity of S. aureus infection is driven by inflammatory responses to the bacteria. Thus, the present study aimed to investigate the modulating role of citral in inflammation caused by S. aureus infection. For this, we used an isolate obtained from a nasal swab sample of a healthy child attending a day-care centre in Vitória da Conquista, Bahia, Brazil. The role of citral in modulating immunological factors against S. aureus infection was evaluated by isolating and cultivating human peripheral blood mononuclear cells. The monocytes were treated with 4%, 2%, and 1% citral before and after inoculation with S. aureus. The cells were analysed by immunophenotyping of monocyte cell surface molecules (CD54, CD282, CD80, HLA-DR, and CD86) and cytokine dosage (IL-1β, IL-6, IL-10, IL-12p70, IL-23, IFN-γ, TGF-β, and TNF-α), and evaluated for the expression of 84 genes related to innate and adaptive immune system responses. GraphPad Prism software and variables with P values < 0.05, were used for statistical analysis. Our data demonstrated citral’s action on the expression of surface markers involved in recognition, presentation, and migration, such as CD14, CD54, and CD80, in global negative regulation of inflammation with inhibitory effects on NF-κB, JNK/p38, and IFN pathways. Consequently, IL-1β, IL-6, IL-12p70, IL-23, IFN-γ, and TNF-α cytokine expression was reduced in groups treated with citral and groups treated with citral at 4%, 2%, and 1% and infected, and levels of anti-inflammatory cytokines such as IL-10 were increased. Furthermore, citral could be used as a supporting anti-inflammatory agent against infections caused by S. aureus. There are no data correlating citral, S. aureus, and the markers analysed here; thus, our study addresses this gap in the literature.
format	article
author	Hellen Braga Martins Oliveira Nathan das Neves Selis Thamara Louisy Santos Brito Beatriz Almeida Sampaio Rafaela de Souza Bittencourt Caline Novais Teixeira Oliveira Manoel Neres Santos Júnior Carolline Florentino Almeida Palloma Porto Almeida Guilherme Barreto Campos Aline Teixeira Amorim Jorge Timenetsky Carla Cristina Romano Ana Paula Trovatti Uetanabaro Regiane Yatsuda Lucas Miranda Marques
author_facet	Hellen Braga Martins Oliveira Nathan das Neves Selis Thamara Louisy Santos Brito Beatriz Almeida Sampaio Rafaela de Souza Bittencourt Caline Novais Teixeira Oliveira Manoel Neres Santos Júnior Carolline Florentino Almeida Palloma Porto Almeida Guilherme Barreto Campos Aline Teixeira Amorim Jorge Timenetsky Carla Cristina Romano Ana Paula Trovatti Uetanabaro Regiane Yatsuda Lucas Miranda Marques
author_sort	Hellen Braga Martins Oliveira
title	Citral modulates human monocyte responses to Staphylococcus aureus infection
title_short	Citral modulates human monocyte responses to Staphylococcus aureus infection
title_full	Citral modulates human monocyte responses to Staphylococcus aureus infection
title_fullStr	Citral modulates human monocyte responses to Staphylococcus aureus infection
title_full_unstemmed	Citral modulates human monocyte responses to Staphylococcus aureus infection
title_sort	citral modulates human monocyte responses to staphylococcus aureus infection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f5e3d110964d44c484a655bfbafd9696
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Citral modulates human monocyte responses to Staphylococcus aureus infection

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