Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells.

Many normal adult tissues contain rare stem cells with extensive self-maintaining regenerative potential. During development, the stem cells of the hematopoietic and neural systems undergo intrinsically specified changes in their self-renewal potential. In the mouse, mammary stem cells with transpla...

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Autores principales:	Maisam Makarem, Nagarajan Kannan, Long V Nguyen, David J H F Knapp, Sneha Balani, Michael D Prater, John Stingl, Afshin Raouf, Oksana Nemirovsky, Peter Eirew, Connie J Eaves
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f5e5ef86b0bd4ec39f0359d8283539c8
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spelling	oai:doaj.org-article:f5e5ef86b0bd4ec39f0359d8283539c82021-11-18T05:37:53ZDevelopmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells.1544-91731545-788510.1371/journal.pbio.1001630https://doaj.org/article/f5e5ef86b0bd4ec39f0359d8283539c82013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23966837/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Many normal adult tissues contain rare stem cells with extensive self-maintaining regenerative potential. During development, the stem cells of the hematopoietic and neural systems undergo intrinsically specified changes in their self-renewal potential. In the mouse, mammary stem cells with transplantable regenerative activity are first detectable a few days before birth. They share some phenotypic properties with their adult counterparts but are enriched in a subpopulation that displays a distinct gene expression profile. Here we show that fetal mammary epithelial cells have a greater direct and inducible growth potential than their adult counterparts. The latter feature is revealed in a novel culture system that enables large numbers of in vitro clonogenic progenitors as well as mammary stem cells with serially transplantable activity to be produced within 7 days from single fetal or adult input cells. We further show that these responses are highly dependent on novel factors produced by fibroblasts. These findings provide new avenues for elucidating mechanisms that regulate normal mammary epithelial stem cell properties at the single-cell level, how these change during development, and how their perturbation may contribute to transformation.Maisam MakaremNagarajan KannanLong V NguyenDavid J H F KnappSneha BalaniMichael D PraterJohn StinglAfshin RaoufOksana NemirovskyPeter EirewConnie J EavesPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 11, Iss 8, p e1001630 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Maisam Makarem Nagarajan Kannan Long V Nguyen David J H F Knapp Sneha Balani Michael D Prater John Stingl Afshin Raouf Oksana Nemirovsky Peter Eirew Connie J Eaves Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells.
description	Many normal adult tissues contain rare stem cells with extensive self-maintaining regenerative potential. During development, the stem cells of the hematopoietic and neural systems undergo intrinsically specified changes in their self-renewal potential. In the mouse, mammary stem cells with transplantable regenerative activity are first detectable a few days before birth. They share some phenotypic properties with their adult counterparts but are enriched in a subpopulation that displays a distinct gene expression profile. Here we show that fetal mammary epithelial cells have a greater direct and inducible growth potential than their adult counterparts. The latter feature is revealed in a novel culture system that enables large numbers of in vitro clonogenic progenitors as well as mammary stem cells with serially transplantable activity to be produced within 7 days from single fetal or adult input cells. We further show that these responses are highly dependent on novel factors produced by fibroblasts. These findings provide new avenues for elucidating mechanisms that regulate normal mammary epithelial stem cell properties at the single-cell level, how these change during development, and how their perturbation may contribute to transformation.
format	article
author	Maisam Makarem Nagarajan Kannan Long V Nguyen David J H F Knapp Sneha Balani Michael D Prater John Stingl Afshin Raouf Oksana Nemirovsky Peter Eirew Connie J Eaves
author_facet	Maisam Makarem Nagarajan Kannan Long V Nguyen David J H F Knapp Sneha Balani Michael D Prater John Stingl Afshin Raouf Oksana Nemirovsky Peter Eirew Connie J Eaves
author_sort	Maisam Makarem
title	Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells.
title_short	Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells.
title_full	Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells.
title_fullStr	Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells.
title_full_unstemmed	Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells.
title_sort	developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f5e5ef86b0bd4ec39f0359d8283539c8
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Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells.

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