Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract

Summary: Visualizing precise spatial patterns of an organ-wide gene and protein expression among diverse cell types can provide critical insights into the fundamental processes underlying normal tissue homeostasis and disease development. Here, we describe an optimized protocol for single-molecule R...

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Autores principales:	Rajendra Kumar Gurumurthy, Naveen Kumar, Cindrilla Chumduri
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Microscopy Gene Expression In Situ Hybridization Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/f5eabe965e014c81a882de798a74ac0d
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spelling	oai:doaj.org-article:f5eabe965e014c81a882de798a74ac0d2021-11-20T05:13:55ZSpatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract2666-166710.1016/j.xpro.2021.100969https://doaj.org/article/f5eabe965e014c81a882de798a74ac0d2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006754https://doaj.org/toc/2666-1667Summary: Visualizing precise spatial patterns of an organ-wide gene and protein expression among diverse cell types can provide critical insights into the fundamental processes underlying normal tissue homeostasis and disease development. Here, we describe an optimized protocol for single-molecule RNA in situ hybridization (smRNA-ISH), immunohistochemistry, and cell lineage analysis of the female reproductive tract organs using commercially available smRNA-ISH probes, antibodies, and inducible Cre-mice. The high-resolution multispectral fluorescence imaging is performed using wide-field epifluorescence or confocal microscopy combined with a slide scanner.For complete details on the use and execution of this protocol, please refer to Chumduri et al. (2021).Rajendra Kumar GurumurthyNaveen KumarCindrilla ChumduriElsevierarticleMicroscopyGene ExpressionIn Situ HybridizationScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100969- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microscopy Gene Expression In Situ Hybridization Science (General) Q1-390
spellingShingle	Microscopy Gene Expression In Situ Hybridization Science (General) Q1-390 Rajendra Kumar Gurumurthy Naveen Kumar Cindrilla Chumduri Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract
description	Summary: Visualizing precise spatial patterns of an organ-wide gene and protein expression among diverse cell types can provide critical insights into the fundamental processes underlying normal tissue homeostasis and disease development. Here, we describe an optimized protocol for single-molecule RNA in situ hybridization (smRNA-ISH), immunohistochemistry, and cell lineage analysis of the female reproductive tract organs using commercially available smRNA-ISH probes, antibodies, and inducible Cre-mice. The high-resolution multispectral fluorescence imaging is performed using wide-field epifluorescence or confocal microscopy combined with a slide scanner.For complete details on the use and execution of this protocol, please refer to Chumduri et al. (2021).
format	article
author	Rajendra Kumar Gurumurthy Naveen Kumar Cindrilla Chumduri
author_facet	Rajendra Kumar Gurumurthy Naveen Kumar Cindrilla Chumduri
author_sort	Rajendra Kumar Gurumurthy
title	Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract
title_short	Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract
title_full	Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract
title_fullStr	Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract
title_full_unstemmed	Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract
title_sort	spatial analysis of organ-wide rna, protein expression, and lineage tracing in the female mouse reproductive tract
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/f5eabe965e014c81a882de798a74ac0d
work_keys_str_mv	AT rajendrakumargurumurthy spatialanalysisoforganwidernaproteinexpressionandlineagetracinginthefemalemousereproductivetract AT naveenkumar spatialanalysisoforganwidernaproteinexpressionandlineagetracinginthefemalemousereproductivetract AT cindrillachumduri spatialanalysisoforganwidernaproteinexpressionandlineagetracinginthefemalemousereproductivetract
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Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract

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