Direct observation of coordinated DNA movements on the nucleosome during chromatin remodelling

Chromatin remodelling enzymes (remodellers) regulate DNA accessibility of eukaryotic genomes, which rely in large part on an ability to reposition nucleosomes. Here the authors use three-colour single-molecule FRET to simultaneously monitor remodeller-induced DNA movements on both sides of the nucle...

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Autores principales: Anton Sabantsev, Robert F. Levendosky, Xiaowei Zhuang, Gregory D. Bowman, Sebastian Deindl
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/f5f376ca46c6431b91508a7dcbf9f536
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Sumario:Chromatin remodelling enzymes (remodellers) regulate DNA accessibility of eukaryotic genomes, which rely in large part on an ability to reposition nucleosomes. Here the authors use three-colour single-molecule FRET to simultaneously monitor remodeller-induced DNA movements on both sides of the nucleosome in real-time.