Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica

Abstract Immune signatures of sheep acutely-infected with Fasciola hepatica, an important pathogen of livestock and humans were analysed within the peritoneal compartment to investigate early infection. Within the peritoneum, F. hepatica antibodies coincided with an intense innate and adaptive cellu...

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Autores principales:	Maria Teresa Ruiz-Campillo, Veronica Molina Hernandez, Alejandro Escamilla, Michael Stevenson, Jose Perez, Alvaro Martinez-Moreno, Sheila Donnelly, John P. Dalton, Krystyna Cwiklinski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f5f5e0d2521442cd88710f4f8231e8fe
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spelling	oai:doaj.org-article:f5f5e0d2521442cd88710f4f8231e8fe2021-12-02T12:32:29ZImmune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica10.1038/s41598-017-03094-02045-2322https://doaj.org/article/f5f5e0d2521442cd88710f4f8231e8fe2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03094-0https://doaj.org/toc/2045-2322Abstract Immune signatures of sheep acutely-infected with Fasciola hepatica, an important pathogen of livestock and humans were analysed within the peritoneal compartment to investigate early infection. Within the peritoneum, F. hepatica antibodies coincided with an intense innate and adaptive cellular immune response, with infiltrating leukocytes and a marked eosinophilia (49%). However, while cytokine qPCR analysis revealed IL-10, IL-12, IL-13, IL-23 and TGFβ were elevated, these were not statistically different at 18 days post-infection compared to uninfected animals indicating that the immune response is muted and not yet skewed to a Th2 type response that is associated with chronic disease. Proteomic analysis of the peritoneal fluid identified infection-related proteins, including several structural proteins derived from the liver extracellular matrix, connective tissue and epithelium, and proteins related to the immune system. Periostin and vascular cell adhesion protein 1 (VCAM-1), molecules that mediate leukocyte infiltration and are associated with inflammatory disorders involving marked eosinophilia (e.g. asthma), were particularly elevated in the peritoneum. Immuno-histochemical studies indicated that the source of periostin and VCAM-1 was the inflamed sheep liver tissue. This study has revealed previously unknown aspects of the immunology and pathogenesis associated with acute fascioliasis in the peritoneum and liver.Maria Teresa Ruiz-CampilloVeronica Molina HernandezAlejandro EscamillaMichael StevensonJose PerezAlvaro Martinez-MorenoSheila DonnellyJohn P. DaltonKrystyna CwiklinskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Teresa Ruiz-Campillo Veronica Molina Hernandez Alejandro Escamilla Michael Stevenson Jose Perez Alvaro Martinez-Moreno Sheila Donnelly John P. Dalton Krystyna Cwiklinski Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica
description	Abstract Immune signatures of sheep acutely-infected with Fasciola hepatica, an important pathogen of livestock and humans were analysed within the peritoneal compartment to investigate early infection. Within the peritoneum, F. hepatica antibodies coincided with an intense innate and adaptive cellular immune response, with infiltrating leukocytes and a marked eosinophilia (49%). However, while cytokine qPCR analysis revealed IL-10, IL-12, IL-13, IL-23 and TGFβ were elevated, these were not statistically different at 18 days post-infection compared to uninfected animals indicating that the immune response is muted and not yet skewed to a Th2 type response that is associated with chronic disease. Proteomic analysis of the peritoneal fluid identified infection-related proteins, including several structural proteins derived from the liver extracellular matrix, connective tissue and epithelium, and proteins related to the immune system. Periostin and vascular cell adhesion protein 1 (VCAM-1), molecules that mediate leukocyte infiltration and are associated with inflammatory disorders involving marked eosinophilia (e.g. asthma), were particularly elevated in the peritoneum. Immuno-histochemical studies indicated that the source of periostin and VCAM-1 was the inflamed sheep liver tissue. This study has revealed previously unknown aspects of the immunology and pathogenesis associated with acute fascioliasis in the peritoneum and liver.
format	article
author	Maria Teresa Ruiz-Campillo Veronica Molina Hernandez Alejandro Escamilla Michael Stevenson Jose Perez Alvaro Martinez-Moreno Sheila Donnelly John P. Dalton Krystyna Cwiklinski
author_facet	Maria Teresa Ruiz-Campillo Veronica Molina Hernandez Alejandro Escamilla Michael Stevenson Jose Perez Alvaro Martinez-Moreno Sheila Donnelly John P. Dalton Krystyna Cwiklinski
author_sort	Maria Teresa Ruiz-Campillo
title	Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica
title_short	Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica
title_full	Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica
title_fullStr	Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica
title_full_unstemmed	Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica
title_sort	immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with fasciola hepatica
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f5f5e0d2521442cd88710f4f8231e8fe
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Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica

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