Gene expression profiles of germ-free and conventional piglets from the same litter

Abstract Germ-free (GF) pigs have clear microbiological backgrounds, and are extensively used as large animal models in the biomedical sciences. However, investigations of the transcriptomic differences between GF and cesarean-derived conventional (CV) piglets are limited. To improve our understandi...

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Autores principales:	Jing Sun, Hang Zhong, Lei Du, Xiaolei Li, Yuchun Ding, Haoran Cao, Zuohua Liu, Liangpeng Ge
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f5f643508bce4202b586f578d05c8517
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spelling	oai:doaj.org-article:f5f643508bce4202b586f578d05c85172021-12-02T11:40:15ZGene expression profiles of germ-free and conventional piglets from the same litter10.1038/s41598-018-29093-32045-2322https://doaj.org/article/f5f643508bce4202b586f578d05c85172018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29093-3https://doaj.org/toc/2045-2322Abstract Germ-free (GF) pigs have clear microbiological backgrounds, and are extensively used as large animal models in the biomedical sciences. However, investigations of the transcriptomic differences between GF and cesarean-derived conventional (CV) piglets are limited. To improve our understanding of GF pigs, and to increase the utility of pigs as an alternative non-rodent model, we used RNA sequencing to profile gene expression in five tissues (the oral mucosae, jejunum, colon, liver, and spleen) of four male GF piglets and four male CV piglets from the same litter. We identified 14 genes that were differentially expressed in all five tissues. Seven of these common differentially expressed genes (DEGs) were interferon-inducible genes, and all 14 were consistently downregulated in the GF piglets as compared to the CV piglets. Compared to the other tissues tested, the expression of transcription factors (TFs) in the colon was most affected by the absence of a microbiota. The expression patterns of immune-related genes were downregulated in the GF piglets as compared to the CV piglets, indicating that the intestinal microbiota influenced gene expression in other tissues besides the gut. Gene Ontology (GO) analysis indicated that, in pigs, the intestinal microbiota affected the expression of genes related to immune system function and development.Jing SunHang ZhongLei DuXiaolei LiYuchun DingHaoran CaoZuohua LiuLiangpeng GeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jing Sun Hang Zhong Lei Du Xiaolei Li Yuchun Ding Haoran Cao Zuohua Liu Liangpeng Ge Gene expression profiles of germ-free and conventional piglets from the same litter
description	Abstract Germ-free (GF) pigs have clear microbiological backgrounds, and are extensively used as large animal models in the biomedical sciences. However, investigations of the transcriptomic differences between GF and cesarean-derived conventional (CV) piglets are limited. To improve our understanding of GF pigs, and to increase the utility of pigs as an alternative non-rodent model, we used RNA sequencing to profile gene expression in five tissues (the oral mucosae, jejunum, colon, liver, and spleen) of four male GF piglets and four male CV piglets from the same litter. We identified 14 genes that were differentially expressed in all five tissues. Seven of these common differentially expressed genes (DEGs) were interferon-inducible genes, and all 14 were consistently downregulated in the GF piglets as compared to the CV piglets. Compared to the other tissues tested, the expression of transcription factors (TFs) in the colon was most affected by the absence of a microbiota. The expression patterns of immune-related genes were downregulated in the GF piglets as compared to the CV piglets, indicating that the intestinal microbiota influenced gene expression in other tissues besides the gut. Gene Ontology (GO) analysis indicated that, in pigs, the intestinal microbiota affected the expression of genes related to immune system function and development.
format	article
author	Jing Sun Hang Zhong Lei Du Xiaolei Li Yuchun Ding Haoran Cao Zuohua Liu Liangpeng Ge
author_facet	Jing Sun Hang Zhong Lei Du Xiaolei Li Yuchun Ding Haoran Cao Zuohua Liu Liangpeng Ge
author_sort	Jing Sun
title	Gene expression profiles of germ-free and conventional piglets from the same litter
title_short	Gene expression profiles of germ-free and conventional piglets from the same litter
title_full	Gene expression profiles of germ-free and conventional piglets from the same litter
title_fullStr	Gene expression profiles of germ-free and conventional piglets from the same litter
title_full_unstemmed	Gene expression profiles of germ-free and conventional piglets from the same litter
title_sort	gene expression profiles of germ-free and conventional piglets from the same litter
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/f5f643508bce4202b586f578d05c8517
work_keys_str_mv	AT jingsun geneexpressionprofilesofgermfreeandconventionalpigletsfromthesamelitter AT hangzhong geneexpressionprofilesofgermfreeandconventionalpigletsfromthesamelitter AT leidu geneexpressionprofilesofgermfreeandconventionalpigletsfromthesamelitter AT xiaoleili geneexpressionprofilesofgermfreeandconventionalpigletsfromthesamelitter AT yuchunding geneexpressionprofilesofgermfreeandconventionalpigletsfromthesamelitter AT haorancao geneexpressionprofilesofgermfreeandconventionalpigletsfromthesamelitter AT zuohualiu geneexpressionprofilesofgermfreeandconventionalpigletsfromthesamelitter AT liangpengge geneexpressionprofilesofgermfreeandconventionalpigletsfromthesamelitter
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Gene expression profiles of germ-free and conventional piglets from the same litter

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