Specific syndecan-1 domains regulate mesenchymal tumor cell adhesion, motility and migration.

<h4>Background</h4>Syndecans are proteoglycans whose core proteins have a short cytoplasmic domain, a transmembrane domain and a large N-terminal extracellular domain possessing glycosaminoglycan chains. Syndecans are involved in many important cellular processes. Our recent publications...

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Autores principales:	Fang Zong, Eleni Fthenou, Filip Mundt, Tünde Szatmári, Ilona Kovalszky, László Szilák, David Brodin, George Tzanakakis, Anders Hjerpe, Katalin Dobra
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f5f6512a30cb403396802922a89cddd3
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spelling	oai:doaj.org-article:f5f6512a30cb403396802922a89cddd32021-11-18T06:51:29ZSpecific syndecan-1 domains regulate mesenchymal tumor cell adhesion, motility and migration.1932-620310.1371/journal.pone.0014816https://doaj.org/article/f5f6512a30cb403396802922a89cddd32011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21731601/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Syndecans are proteoglycans whose core proteins have a short cytoplasmic domain, a transmembrane domain and a large N-terminal extracellular domain possessing glycosaminoglycan chains. Syndecans are involved in many important cellular processes. Our recent publications have demonstrated that syndecan-1 translocates into the nucleus and hampers tumor cell proliferation. In the present study, we aimed to investigate the role of syndecan-1 in tumor cell adhesion and migration, with special focus on the importance of its distinct protein domains, to better understand the structure-function relationship of syndecan-1 in tumor progression.<h4>Methodology/principal findings</h4>We utilized two mesenchymal tumor cell lines which were transfected to stably overexpress full-length syndecan-1 or truncated variants: the 78 which lacks the extracellular domain except the DRKE sequence proposed to be essential for oligomerization, the 77 which lacks the whole extracellular domain, and the RMKKK which serves as a nuclear localization signal. The deletion of the RMKKK motif from full-length syndecan-1 abolished the nuclear translocation of this proteoglycan. Various bioassays for cell adhesion, chemotaxis, random movement and wound healing were studied. Furthermore, we performed gene microarray to analyze the global gene expression pattern influenced by syndecan-1. Both full-length and truncated syndecan-1 constructs decrease tumor cell migration and motility, and affect cell adhesion. Distinct protein domains have differential effects, the extracellular domain is more important for promoting cell adhesion, while the transmembrane and cytoplasmic domains are sufficient for inhibition of cell migration. Cell behavior seems to depend also on the nuclear translocation of syndecan-1. Many genes are differentially regulated by syndecan-1 and a number of genes are actually involved in cell adhesion and migration.<h4>Conclusions/significance</h4>Our results demonstrate that syndecan-1 regulates mesenchymal tumor cell adhesion and migration, and different domains have differential effects. Our study provides new insights into better understanding of the role of syndecans in tumor progression.Fang ZongEleni FthenouFilip MundtTünde SzatmáriIlona KovalszkyLászló SzilákDavid BrodinGeorge TzanakakisAnders HjerpeKatalin DobraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 6, p e14816 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fang Zong Eleni Fthenou Filip Mundt Tünde Szatmári Ilona Kovalszky László Szilák David Brodin George Tzanakakis Anders Hjerpe Katalin Dobra Specific syndecan-1 domains regulate mesenchymal tumor cell adhesion, motility and migration.
description	<h4>Background</h4>Syndecans are proteoglycans whose core proteins have a short cytoplasmic domain, a transmembrane domain and a large N-terminal extracellular domain possessing glycosaminoglycan chains. Syndecans are involved in many important cellular processes. Our recent publications have demonstrated that syndecan-1 translocates into the nucleus and hampers tumor cell proliferation. In the present study, we aimed to investigate the role of syndecan-1 in tumor cell adhesion and migration, with special focus on the importance of its distinct protein domains, to better understand the structure-function relationship of syndecan-1 in tumor progression.<h4>Methodology/principal findings</h4>We utilized two mesenchymal tumor cell lines which were transfected to stably overexpress full-length syndecan-1 or truncated variants: the 78 which lacks the extracellular domain except the DRKE sequence proposed to be essential for oligomerization, the 77 which lacks the whole extracellular domain, and the RMKKK which serves as a nuclear localization signal. The deletion of the RMKKK motif from full-length syndecan-1 abolished the nuclear translocation of this proteoglycan. Various bioassays for cell adhesion, chemotaxis, random movement and wound healing were studied. Furthermore, we performed gene microarray to analyze the global gene expression pattern influenced by syndecan-1. Both full-length and truncated syndecan-1 constructs decrease tumor cell migration and motility, and affect cell adhesion. Distinct protein domains have differential effects, the extracellular domain is more important for promoting cell adhesion, while the transmembrane and cytoplasmic domains are sufficient for inhibition of cell migration. Cell behavior seems to depend also on the nuclear translocation of syndecan-1. Many genes are differentially regulated by syndecan-1 and a number of genes are actually involved in cell adhesion and migration.<h4>Conclusions/significance</h4>Our results demonstrate that syndecan-1 regulates mesenchymal tumor cell adhesion and migration, and different domains have differential effects. Our study provides new insights into better understanding of the role of syndecans in tumor progression.
format	article
author	Fang Zong Eleni Fthenou Filip Mundt Tünde Szatmári Ilona Kovalszky László Szilák David Brodin George Tzanakakis Anders Hjerpe Katalin Dobra
author_facet	Fang Zong Eleni Fthenou Filip Mundt Tünde Szatmári Ilona Kovalszky László Szilák David Brodin George Tzanakakis Anders Hjerpe Katalin Dobra
author_sort	Fang Zong
title	Specific syndecan-1 domains regulate mesenchymal tumor cell adhesion, motility and migration.
title_short	Specific syndecan-1 domains regulate mesenchymal tumor cell adhesion, motility and migration.
title_full	Specific syndecan-1 domains regulate mesenchymal tumor cell adhesion, motility and migration.
title_fullStr	Specific syndecan-1 domains regulate mesenchymal tumor cell adhesion, motility and migration.
title_full_unstemmed	Specific syndecan-1 domains regulate mesenchymal tumor cell adhesion, motility and migration.
title_sort	specific syndecan-1 domains regulate mesenchymal tumor cell adhesion, motility and migration.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/f5f6512a30cb403396802922a89cddd3
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_version_	1718424329682681856

Specific syndecan-1 domains regulate mesenchymal tumor cell adhesion, motility and migration.

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