Towards endometriosis diagnosis by gadofosveset-trisodium enhanced magnetic resonance imaging.

Endometriosis is defined as the presence of endometrial tissue outside the uterus. It affects 10-15% of women during reproductive age and has a big personal and social impact due to chronic pelvic pain, subfertility, loss of work-hours and medical costs. Such conditions are exacerbated by the fact t...

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Autores principales:	Marc H Schreinemacher, Walter H Backes, Jos M Slenter, Sofia Xanthoulea, Bert Delvoux, Larissa van Winden, Regina G Beets-Tan, Johannes L H Evers, Gerard A J Dunselman, Andrea Romano
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f5fb2e8550154cd19f39ec5ae17c99fe
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spelling	oai:doaj.org-article:f5fb2e8550154cd19f39ec5ae17c99fe2021-11-18T07:24:28ZTowards endometriosis diagnosis by gadofosveset-trisodium enhanced magnetic resonance imaging.1932-620310.1371/journal.pone.0033241https://doaj.org/article/f5fb2e8550154cd19f39ec5ae17c99fe2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22457748/?tool=EBIhttps://doaj.org/toc/1932-6203Endometriosis is defined as the presence of endometrial tissue outside the uterus. It affects 10-15% of women during reproductive age and has a big personal and social impact due to chronic pelvic pain, subfertility, loss of work-hours and medical costs. Such conditions are exacerbated by the fact that the correct diagnosis is made as late as 8-11 years after symptom presentation. This is due to the lack of a reliable non-invasive diagnostic test and the fact that the reference diagnostic standard is laparoscopy (invasive, expensive and not without risks). High-molecular weight gadofosveset-trisodium is used as contrast agent in Magnetic Resonance Imaging (MRI). Since it extravasates from hyperpermeable vessels more easily than from mature blood vessels, this contrast agent detects angiogenesis efficiently. Endometriosis has high angiogenic activity. Therefore, we have tested the possibility to detect endometriosis non-invasively using Dynamic Contrast-Enhanced MRI (DCE-MRI) and gadofosveset-trisodium as a contrast agent in a mouse model. Endometriotic lesions were surgically induced in nine mice by autologous transplantation. Three weeks after lesion induction, mice were scanned by DCE-MRI. Dynamic image analysis showed that the rates of uptake (inwash), persistence and outwash of the contrast agent were different between endometriosis and control tissues (large blood vessels and back muscle). Due to the extensive angiogenesis in induced lesions, the contrast agent persisted longer in endometriotic than control tissues, thus enhancing the MRI signal intensity. DCE-MRI was repeated five weeks after lesion induction, and contrast enhancement was similar to that observed three weeks after endometriosis induction. The endothelial-cell marker CD31 and the pericyte marker α-smooth-muscle-actin (mature vessels) were detected with immunohistochemistry and confirmed that endometriotic lesions had significantly higher prevalence of new vessels (CD31 only positive) than the uterus and control tissues. The diagnostic value of gadofosveset-trisodium to detect endometriosis should be tested in human settings.Marc H SchreinemacherWalter H BackesJos M SlenterSofia XanthouleaBert DelvouxLarissa van WindenRegina G Beets-TanJohannes L H EversGerard A J DunselmanAndrea RomanoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e33241 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marc H Schreinemacher Walter H Backes Jos M Slenter Sofia Xanthoulea Bert Delvoux Larissa van Winden Regina G Beets-Tan Johannes L H Evers Gerard A J Dunselman Andrea Romano Towards endometriosis diagnosis by gadofosveset-trisodium enhanced magnetic resonance imaging.
description	Endometriosis is defined as the presence of endometrial tissue outside the uterus. It affects 10-15% of women during reproductive age and has a big personal and social impact due to chronic pelvic pain, subfertility, loss of work-hours and medical costs. Such conditions are exacerbated by the fact that the correct diagnosis is made as late as 8-11 years after symptom presentation. This is due to the lack of a reliable non-invasive diagnostic test and the fact that the reference diagnostic standard is laparoscopy (invasive, expensive and not without risks). High-molecular weight gadofosveset-trisodium is used as contrast agent in Magnetic Resonance Imaging (MRI). Since it extravasates from hyperpermeable vessels more easily than from mature blood vessels, this contrast agent detects angiogenesis efficiently. Endometriosis has high angiogenic activity. Therefore, we have tested the possibility to detect endometriosis non-invasively using Dynamic Contrast-Enhanced MRI (DCE-MRI) and gadofosveset-trisodium as a contrast agent in a mouse model. Endometriotic lesions were surgically induced in nine mice by autologous transplantation. Three weeks after lesion induction, mice were scanned by DCE-MRI. Dynamic image analysis showed that the rates of uptake (inwash), persistence and outwash of the contrast agent were different between endometriosis and control tissues (large blood vessels and back muscle). Due to the extensive angiogenesis in induced lesions, the contrast agent persisted longer in endometriotic than control tissues, thus enhancing the MRI signal intensity. DCE-MRI was repeated five weeks after lesion induction, and contrast enhancement was similar to that observed three weeks after endometriosis induction. The endothelial-cell marker CD31 and the pericyte marker α-smooth-muscle-actin (mature vessels) were detected with immunohistochemistry and confirmed that endometriotic lesions had significantly higher prevalence of new vessels (CD31 only positive) than the uterus and control tissues. The diagnostic value of gadofosveset-trisodium to detect endometriosis should be tested in human settings.
format	article
author	Marc H Schreinemacher Walter H Backes Jos M Slenter Sofia Xanthoulea Bert Delvoux Larissa van Winden Regina G Beets-Tan Johannes L H Evers Gerard A J Dunselman Andrea Romano
author_facet	Marc H Schreinemacher Walter H Backes Jos M Slenter Sofia Xanthoulea Bert Delvoux Larissa van Winden Regina G Beets-Tan Johannes L H Evers Gerard A J Dunselman Andrea Romano
author_sort	Marc H Schreinemacher
title	Towards endometriosis diagnosis by gadofosveset-trisodium enhanced magnetic resonance imaging.
title_short	Towards endometriosis diagnosis by gadofosveset-trisodium enhanced magnetic resonance imaging.
title_full	Towards endometriosis diagnosis by gadofosveset-trisodium enhanced magnetic resonance imaging.
title_fullStr	Towards endometriosis diagnosis by gadofosveset-trisodium enhanced magnetic resonance imaging.
title_full_unstemmed	Towards endometriosis diagnosis by gadofosveset-trisodium enhanced magnetic resonance imaging.
title_sort	towards endometriosis diagnosis by gadofosveset-trisodium enhanced magnetic resonance imaging.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/f5fb2e8550154cd19f39ec5ae17c99fe
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Towards endometriosis diagnosis by gadofosveset-trisodium enhanced magnetic resonance imaging.

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