Recombinant HcGAPDH Protein Expressed on Probiotic <italic toggle="yes">Bacillus subtilis</italic> Spores Protects Sheep from <italic toggle="yes">Haemonchus contortus</italic> Infection by Inducing both Humoral and Cell-Mediated Responses

ABSTRACT Probiotic Bacillales are effective in controlling pathogens. Live probiotic bacteria improve the composition of the gastrointestinal microbiota, leading to a reduction in pathogen colonization. However, it remains largely unknown how probiotics regulate the host’s immunologic responses and...

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Autores principales:	Yi Yang, Guiheng Zhang, Jie Wu, Xueqiu Chen, Danni Tong, Yimin Yang, Hengzhi Shi, Chaoqun Yao, Lenan Zhuang, Jianbin Wang, Aifang Du
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Bacillus subtilis Haemonchus contortus spore coat microbiota recombinant live vaccine Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f5fd5f177d78452ab790059fade5cfce
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spelling	oai:doaj.org-article:f5fd5f177d78452ab790059fade5cfce2021-12-02T18:44:35ZRecombinant HcGAPDH Protein Expressed on Probiotic <italic toggle="yes">Bacillus subtilis</italic> Spores Protects Sheep from <italic toggle="yes">Haemonchus contortus</italic> Infection by Inducing both Humoral and Cell-Mediated Responses10.1128/mSystems.00239-202379-5077https://doaj.org/article/f5fd5f177d78452ab790059fade5cfce2020-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00239-20https://doaj.org/toc/2379-5077ABSTRACT Probiotic Bacillales are effective in controlling pathogens. Live probiotic bacteria improve the composition of the gastrointestinal microbiota, leading to a reduction in pathogen colonization. However, it remains largely unknown how probiotics regulate the host’s immunologic responses and protect the host from parasitic infection. In this study, we addressed whether Bacillales were effective against Haemonchus contortus, a parasitic nematode that infects small ruminants worldwide. Using 16S rRNA sequencing, we found that Bacillales were largely depleted in the abomasal microbiota of sheep infected with H. contortus. We constructed a recombinant Bacillus subtilis named rBSCotB-HcG that express the glyceraldehyde-3-phosphate dehydrogenase of H. contortus (HcGAPDH) on its spore surface using the Bacillus subtilis spore coat protein B (CotB) as a carrier. Mice receiving rBSCotB-HcG orally showed strong Th1-dominated immune responses. More importantly, sheep administered BSCotB-HcG per os showed increasing proliferation of the peripheral blood mononucleates, elevated anti-HcGAPDH IgG in sera, and higher anti-HcGAPDH sIgA in the intestinal mucus than the control sheep. The average weight gain of H. contortus-infected sheep treated with rBSCotB-HcG (Hc+rBSCotB-HcG) was 48.73% greater than that of unvaccinated sheep. Furthermore, these Hc+rBSCotB-HcG sheep had fewer eggs per gram of feces by 84.1% and adult worms by 71.5%. They also demonstrated greatly lessened abomasal damage by H. contortus with an abundance of probiotic species in the abomasal microbiota. Collectively, our data unequivocally demonstrate the protective roles of CotB-HcGAPDH-expressing B. subtilis spores in against H. contortus infection and showed great potential of using probiotic-based strategy in controlling parasitic nematodes of socioeconomic importance in general. IMPORTANCE Initial analyses of the abomasal microbiota of sheep using 16S rRNA sequencing suggested that probiotic bacteria played a protective role in against H. contortus infection. A recombinant Bacillus subtilis expressing a fusion protein CotB-HcGAPDH on its spore’s surface induced strong Th1 immune response in a murine model. The same probiotic recombinant, upon only one oral application, protected sheep against H. contortus infection by reducing egg shedding and decreasing adult worm loads of the parasite and increasing body weight gain of infected sheep. Both Th1 and Th2 immune responses were evident in these immunized sheep.Yi YangGuiheng ZhangJie WuXueqiu ChenDanni TongYimin YangHengzhi ShiChaoqun YaoLenan ZhuangJianbin WangAifang DuAmerican Society for MicrobiologyarticleBacillus subtilisHaemonchus contortusspore coatmicrobiotarecombinant live vaccineMicrobiologyQR1-502ENmSystems, Vol 5, Iss 3 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bacillus subtilis Haemonchus contortus spore coat microbiota recombinant live vaccine Microbiology QR1-502
spellingShingle	Bacillus subtilis Haemonchus contortus spore coat microbiota recombinant live vaccine Microbiology QR1-502 Yi Yang Guiheng Zhang Jie Wu Xueqiu Chen Danni Tong Yimin Yang Hengzhi Shi Chaoqun Yao Lenan Zhuang Jianbin Wang Aifang Du Recombinant HcGAPDH Protein Expressed on Probiotic <italic toggle="yes">Bacillus subtilis</italic> Spores Protects Sheep from <italic toggle="yes">Haemonchus contortus</italic> Infection by Inducing both Humoral and Cell-Mediated Responses
description	ABSTRACT Probiotic Bacillales are effective in controlling pathogens. Live probiotic bacteria improve the composition of the gastrointestinal microbiota, leading to a reduction in pathogen colonization. However, it remains largely unknown how probiotics regulate the host’s immunologic responses and protect the host from parasitic infection. In this study, we addressed whether Bacillales were effective against Haemonchus contortus, a parasitic nematode that infects small ruminants worldwide. Using 16S rRNA sequencing, we found that Bacillales were largely depleted in the abomasal microbiota of sheep infected with H. contortus. We constructed a recombinant Bacillus subtilis named rBSCotB-HcG that express the glyceraldehyde-3-phosphate dehydrogenase of H. contortus (HcGAPDH) on its spore surface using the Bacillus subtilis spore coat protein B (CotB) as a carrier. Mice receiving rBSCotB-HcG orally showed strong Th1-dominated immune responses. More importantly, sheep administered BSCotB-HcG per os showed increasing proliferation of the peripheral blood mononucleates, elevated anti-HcGAPDH IgG in sera, and higher anti-HcGAPDH sIgA in the intestinal mucus than the control sheep. The average weight gain of H. contortus-infected sheep treated with rBSCotB-HcG (Hc+rBSCotB-HcG) was 48.73% greater than that of unvaccinated sheep. Furthermore, these Hc+rBSCotB-HcG sheep had fewer eggs per gram of feces by 84.1% and adult worms by 71.5%. They also demonstrated greatly lessened abomasal damage by H. contortus with an abundance of probiotic species in the abomasal microbiota. Collectively, our data unequivocally demonstrate the protective roles of CotB-HcGAPDH-expressing B. subtilis spores in against H. contortus infection and showed great potential of using probiotic-based strategy in controlling parasitic nematodes of socioeconomic importance in general. IMPORTANCE Initial analyses of the abomasal microbiota of sheep using 16S rRNA sequencing suggested that probiotic bacteria played a protective role in against H. contortus infection. A recombinant Bacillus subtilis expressing a fusion protein CotB-HcGAPDH on its spore’s surface induced strong Th1 immune response in a murine model. The same probiotic recombinant, upon only one oral application, protected sheep against H. contortus infection by reducing egg shedding and decreasing adult worm loads of the parasite and increasing body weight gain of infected sheep. Both Th1 and Th2 immune responses were evident in these immunized sheep.
format	article
author	Yi Yang Guiheng Zhang Jie Wu Xueqiu Chen Danni Tong Yimin Yang Hengzhi Shi Chaoqun Yao Lenan Zhuang Jianbin Wang Aifang Du
author_facet	Yi Yang Guiheng Zhang Jie Wu Xueqiu Chen Danni Tong Yimin Yang Hengzhi Shi Chaoqun Yao Lenan Zhuang Jianbin Wang Aifang Du
author_sort	Yi Yang
title	Recombinant HcGAPDH Protein Expressed on Probiotic <italic toggle="yes">Bacillus subtilis</italic> Spores Protects Sheep from <italic toggle="yes">Haemonchus contortus</italic> Infection by Inducing both Humoral and Cell-Mediated Responses
title_short	Recombinant HcGAPDH Protein Expressed on Probiotic <italic toggle="yes">Bacillus subtilis</italic> Spores Protects Sheep from <italic toggle="yes">Haemonchus contortus</italic> Infection by Inducing both Humoral and Cell-Mediated Responses
title_full	Recombinant HcGAPDH Protein Expressed on Probiotic <italic toggle="yes">Bacillus subtilis</italic> Spores Protects Sheep from <italic toggle="yes">Haemonchus contortus</italic> Infection by Inducing both Humoral and Cell-Mediated Responses
title_fullStr	Recombinant HcGAPDH Protein Expressed on Probiotic <italic toggle="yes">Bacillus subtilis</italic> Spores Protects Sheep from <italic toggle="yes">Haemonchus contortus</italic> Infection by Inducing both Humoral and Cell-Mediated Responses
title_full_unstemmed	Recombinant HcGAPDH Protein Expressed on Probiotic <italic toggle="yes">Bacillus subtilis</italic> Spores Protects Sheep from <italic toggle="yes">Haemonchus contortus</italic> Infection by Inducing both Humoral and Cell-Mediated Responses
title_sort	recombinant hcgapdh protein expressed on probiotic <italic toggle="yes">bacillus subtilis</italic> spores protects sheep from <italic toggle="yes">haemonchus contortus</italic> infection by inducing both humoral and cell-mediated responses
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/f5fd5f177d78452ab790059fade5cfce
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Recombinant HcGAPDH Protein Expressed on Probiotic <italic toggle="yes">Bacillus subtilis</italic> Spores Protects Sheep from <italic toggle="yes">Haemonchus contortus</italic> Infection by Inducing both Humoral and Cell-Mediated Responses

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