Organ-specific, integrated omics data-based study on the metabolic pathways of the medicinal plant Bletilla striata (Orchidaceae)

Abstract Background Bletilla striata is one of the important species belonging to the Bletilla genus of Orchidaceae. Since its extracts have an astringent effect on human tissues, B. striata is widely used for hemostasis and healing. Recently, some other beneficial effects have also been uncovered,...

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Autores principales:	Xiaoxia Ma, Kehua Tang, Zhonghai Tang, Aiwen Dong, Yijun Meng, Pu Wang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Organ-specific Transcriptome assembly Metabolic pathways Gene expression Long-range transportation Bletilla striata Botany QK1-989
Acceso en línea:	https://doaj.org/article/f60605af60134132b1d6fdc1ff13ce7a
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spelling	oai:doaj.org-article:f60605af60134132b1d6fdc1ff13ce7a2021-11-07T12:09:25ZOrgan-specific, integrated omics data-based study on the metabolic pathways of the medicinal plant Bletilla striata (Orchidaceae)10.1186/s12870-021-03288-91471-2229https://doaj.org/article/f60605af60134132b1d6fdc1ff13ce7a2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12870-021-03288-9https://doaj.org/toc/1471-2229Abstract Background Bletilla striata is one of the important species belonging to the Bletilla genus of Orchidaceae. Since its extracts have an astringent effect on human tissues, B. striata is widely used for hemostasis and healing. Recently, some other beneficial effects have also been uncovered, such as antioxidation, antiinflammation, antifibrotic, and immunomodulatory activities. As a key step towards a thorough understanding on the medicinal ingredient production in B. striata, deciphering the regulatory codes of the metabolic pathways becomes a major task. Results In this study, three organs (roots, tubers and leaves) of B. striata were analyzed by integrating transcriptome sequencing and untargeted metabolic profiling data. Five different metabolic pathways, involved in polysaccharide, sterol, flavonoid, terpenoid and alkaloid biosynthesis, were investigated respectively. For each pathway, the expression patterns of the enzyme-coding genes and the accumulation levels of the metabolic intermediates were presented in an organ-specific way. Furthermore, the relationships between enzyme activities and the levels of the related metabolites were partially inferred. Within the biosynthetic pathways of polysaccharides and flavonoids, long-range phytochemical transportation was proposed for certain metabolic intermediates and/or the enzymes. Conclusions The data presented by this work could strengthen the molecular basis for further studies on breeding and medicinal uses of B. striata.Xiaoxia MaKehua TangZhonghai TangAiwen DongYijun MengPu WangBMCarticleOrgan-specificTranscriptome assemblyMetabolic pathwaysGene expressionLong-range transportationBletilla striataBotanyQK1-989ENBMC Plant Biology, Vol 21, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Organ-specific Transcriptome assembly Metabolic pathways Gene expression Long-range transportation Bletilla striata Botany QK1-989
spellingShingle	Organ-specific Transcriptome assembly Metabolic pathways Gene expression Long-range transportation Bletilla striata Botany QK1-989 Xiaoxia Ma Kehua Tang Zhonghai Tang Aiwen Dong Yijun Meng Pu Wang Organ-specific, integrated omics data-based study on the metabolic pathways of the medicinal plant Bletilla striata (Orchidaceae)
description	Abstract Background Bletilla striata is one of the important species belonging to the Bletilla genus of Orchidaceae. Since its extracts have an astringent effect on human tissues, B. striata is widely used for hemostasis and healing. Recently, some other beneficial effects have also been uncovered, such as antioxidation, antiinflammation, antifibrotic, and immunomodulatory activities. As a key step towards a thorough understanding on the medicinal ingredient production in B. striata, deciphering the regulatory codes of the metabolic pathways becomes a major task. Results In this study, three organs (roots, tubers and leaves) of B. striata were analyzed by integrating transcriptome sequencing and untargeted metabolic profiling data. Five different metabolic pathways, involved in polysaccharide, sterol, flavonoid, terpenoid and alkaloid biosynthesis, were investigated respectively. For each pathway, the expression patterns of the enzyme-coding genes and the accumulation levels of the metabolic intermediates were presented in an organ-specific way. Furthermore, the relationships between enzyme activities and the levels of the related metabolites were partially inferred. Within the biosynthetic pathways of polysaccharides and flavonoids, long-range phytochemical transportation was proposed for certain metabolic intermediates and/or the enzymes. Conclusions The data presented by this work could strengthen the molecular basis for further studies on breeding and medicinal uses of B. striata.
format	article
author	Xiaoxia Ma Kehua Tang Zhonghai Tang Aiwen Dong Yijun Meng Pu Wang
author_facet	Xiaoxia Ma Kehua Tang Zhonghai Tang Aiwen Dong Yijun Meng Pu Wang
author_sort	Xiaoxia Ma
title	Organ-specific, integrated omics data-based study on the metabolic pathways of the medicinal plant Bletilla striata (Orchidaceae)
title_short	Organ-specific, integrated omics data-based study on the metabolic pathways of the medicinal plant Bletilla striata (Orchidaceae)
title_full	Organ-specific, integrated omics data-based study on the metabolic pathways of the medicinal plant Bletilla striata (Orchidaceae)
title_fullStr	Organ-specific, integrated omics data-based study on the metabolic pathways of the medicinal plant Bletilla striata (Orchidaceae)
title_full_unstemmed	Organ-specific, integrated omics data-based study on the metabolic pathways of the medicinal plant Bletilla striata (Orchidaceae)
title_sort	organ-specific, integrated omics data-based study on the metabolic pathways of the medicinal plant bletilla striata (orchidaceae)
publisher	BMC
publishDate	2021
url	https://doaj.org/article/f60605af60134132b1d6fdc1ff13ce7a
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Organ-specific, integrated omics data-based study on the metabolic pathways of the medicinal plant Bletilla striata (Orchidaceae)

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