Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons.
Signaling via NF-κB in neurons depends on complex formation with interactors such as dynein/dynactin motor complex and can be triggered by synaptic activation. However, so far a detailed interaction map for the neuronal NF-κB is missing. In this study we used mass spectrometry to identify novel inte...
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2013
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oai:doaj.org-article:f61d7f07f49e49418e8eb961bd88150b2021-11-18T07:42:38ZHsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons.1932-620310.1371/journal.pone.0065280https://doaj.org/article/f61d7f07f49e49418e8eb961bd88150b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23762333/?tool=EBIhttps://doaj.org/toc/1932-6203Signaling via NF-κB in neurons depends on complex formation with interactors such as dynein/dynactin motor complex and can be triggered by synaptic activation. However, so far a detailed interaction map for the neuronal NF-κB is missing. In this study we used mass spectrometry to identify novel interactors of NF-κB p65 within the brain. Hsc70 was identified as a novel neuronal interactor of NF-κB p65. In HEK293 cells, a direct physical interaction was shown by co-immunoprecipitation and verified via in situ proximity ligation in healthy rat neurons. Pharmacological blockade of Hsc70 by deoxyspergualin (DSG) strongly decreased nuclear translocation of NF-κB p65 and transcriptional activity shown by reporter gene assays in neurons after stimulation with glutamate. In addition, knock down of Hsc70 via siRNA significantly reduced neuronal NF-κB activity. Taken together these data provide evidence for Hsc70 as a novel neuronal interactor of NF-κB p65.Christin KlenkeDarius WideraThomas EngelenJanine MüllerThomas NollKarsten NiehausM Lienhard SchmitzBarbara KaltschmidtChristian KaltschmidtPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e65280 (2013) |
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EN |
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Medicine R Science Q Christin Klenke Darius Widera Thomas Engelen Janine Müller Thomas Noll Karsten Niehaus M Lienhard Schmitz Barbara Kaltschmidt Christian Kaltschmidt Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. |
| description |
Signaling via NF-κB in neurons depends on complex formation with interactors such as dynein/dynactin motor complex and can be triggered by synaptic activation. However, so far a detailed interaction map for the neuronal NF-κB is missing. In this study we used mass spectrometry to identify novel interactors of NF-κB p65 within the brain. Hsc70 was identified as a novel neuronal interactor of NF-κB p65. In HEK293 cells, a direct physical interaction was shown by co-immunoprecipitation and verified via in situ proximity ligation in healthy rat neurons. Pharmacological blockade of Hsc70 by deoxyspergualin (DSG) strongly decreased nuclear translocation of NF-κB p65 and transcriptional activity shown by reporter gene assays in neurons after stimulation with glutamate. In addition, knock down of Hsc70 via siRNA significantly reduced neuronal NF-κB activity. Taken together these data provide evidence for Hsc70 as a novel neuronal interactor of NF-κB p65. |
| format |
article |
| author |
Christin Klenke Darius Widera Thomas Engelen Janine Müller Thomas Noll Karsten Niehaus M Lienhard Schmitz Barbara Kaltschmidt Christian Kaltschmidt |
| author_facet |
Christin Klenke Darius Widera Thomas Engelen Janine Müller Thomas Noll Karsten Niehaus M Lienhard Schmitz Barbara Kaltschmidt Christian Kaltschmidt |
| author_sort |
Christin Klenke |
| title |
Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. |
| title_short |
Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. |
| title_full |
Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. |
| title_fullStr |
Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. |
| title_full_unstemmed |
Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. |
| title_sort |
hsc70 is a novel interactor of nf-kappab p65 in living hippocampal neurons. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/f61d7f07f49e49418e8eb961bd88150b |
| work_keys_str_mv |
AT christinklenke hsc70isanovelinteractorofnfkappabp65inlivinghippocampalneurons AT dariuswidera hsc70isanovelinteractorofnfkappabp65inlivinghippocampalneurons AT thomasengelen hsc70isanovelinteractorofnfkappabp65inlivinghippocampalneurons AT janinemuller hsc70isanovelinteractorofnfkappabp65inlivinghippocampalneurons AT thomasnoll hsc70isanovelinteractorofnfkappabp65inlivinghippocampalneurons AT karstenniehaus hsc70isanovelinteractorofnfkappabp65inlivinghippocampalneurons AT mlienhardschmitz hsc70isanovelinteractorofnfkappabp65inlivinghippocampalneurons AT barbarakaltschmidt hsc70isanovelinteractorofnfkappabp65inlivinghippocampalneurons AT christiankaltschmidt hsc70isanovelinteractorofnfkappabp65inlivinghippocampalneurons |
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