Nanoporous diopside modulates biocompatibility, degradability and osteogenesis of bioactive scaffolds of gliadin-based composites for new bone formation

Zhaoyu Ba,1,* Zhaoxiong Chen,1,* Yufeng Huang,1,* Du Feng,2 Qinghui Zhao,3 Jianguang Zhu,1 Desheng Wu1 1Department of Spinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China; 2Key Laboratory of Protein Modification and Degradation, School of Basic Medical Scien...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ba Z, Chen Z, Huang Y, Feng D, Zhao Q, Zhu J, Wu D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	nanoporous diopside bioglass gliadin composite scaffolds degradability osteogenesis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f624134a4cd0441d9f913b642a8234bf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f624134a4cd0441d9f913b642a8234bf
record_format	dspace
spelling	oai:doaj.org-article:f624134a4cd0441d9f913b642a8234bf2021-12-02T05:02:48ZNanoporous diopside modulates biocompatibility, degradability and osteogenesis of bioactive scaffolds of gliadin-based composites for new bone formation1178-2013https://doaj.org/article/f624134a4cd0441d9f913b642a8234bf2018-07-01T00:00:00Zhttps://www.dovepress.com/nanoporous-diopside-modulates-biocompatibility-degradability-and-osteo-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhaoyu Ba,1,* Zhaoxiong Chen,1,* Yufeng Huang,1,* Du Feng,2 Qinghui Zhao,3 Jianguang Zhu,1 Desheng Wu1 1Department of Spinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China; 2Key Laboratory of Protein Modification and Degradation, School of Basic Medical Sciences, Affiliated Cancer Hospital, Institute of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China; 3Biobank, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China *These authors contributed equally to this work Introduction: It is predicted that with increased life expectancy in the whole world, there will be a greater demand for synthetic biomedical materials to repair or regenerate lost, injured or diseased tissues. Natural polymers, as biomedical materials, have been widely applied in the field of regenerative medicine. Materials and methods: By incorporation of nanoporous diopside bioglass (nDPB) into gliadin (GL) matrix, macro–nanoporous scaffolds of nDPB/GL composites (DGC) were fabricated by method of solution compressing and particles leaching. Results: The results revealed that the DGC scaffolds possessed well-interconnected macropores of 200–500 µm and nanopores of 4 nm, and the porosity and degradability of DGC scaffolds remarkably increased with the increase in nDPB content. In addition, in vitro cell experiments revealed that the adhesion and growth of MC3T3-E1 cells on DGC scaffolds were significantly promoted, which depended on nDPB content. Moreover, the results of histological evaluations confirmed that the osteogenic properties and degradability of DGC scaffolds in vivo significantly improved, which were nDPB content dependent. Furthermore, the results of immunohistochemical analysis demonstrated that, with the increase in nDPB content, the type I collagen expression in DGC scaffolds in vivo obviously enhanced, indicating excellent osteogenesis. Discussion and conclusion: The results demonstrated that the DGC scaffolds containing 30 wt% nDPB (30nDGC) exhibited good biocompatibility and new bone formation ability, which might have a great potential for applications in bone regeneration. Keywords: nanoporous diopside bioglass, gliadin, macro-nanoporous, MC3T3-E1, osteogenesisBa ZChen ZHuang YFeng DZhao QZhu JWu DDove Medical Pressarticlenanoporous diopside bioglassgliadincomposite scaffoldsdegradabilityosteogenesisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 3883-3896 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanoporous diopside bioglass gliadin composite scaffolds degradability osteogenesis Medicine (General) R5-920
spellingShingle	nanoporous diopside bioglass gliadin composite scaffolds degradability osteogenesis Medicine (General) R5-920 Ba Z Chen Z Huang Y Feng D Zhao Q Zhu J Wu D Nanoporous diopside modulates biocompatibility, degradability and osteogenesis of bioactive scaffolds of gliadin-based composites for new bone formation
description	Zhaoyu Ba,1,* Zhaoxiong Chen,1,* Yufeng Huang,1,* Du Feng,2 Qinghui Zhao,3 Jianguang Zhu,1 Desheng Wu1 1Department of Spinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China; 2Key Laboratory of Protein Modification and Degradation, School of Basic Medical Sciences, Affiliated Cancer Hospital, Institute of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China; 3Biobank, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China *These authors contributed equally to this work Introduction: It is predicted that with increased life expectancy in the whole world, there will be a greater demand for synthetic biomedical materials to repair or regenerate lost, injured or diseased tissues. Natural polymers, as biomedical materials, have been widely applied in the field of regenerative medicine. Materials and methods: By incorporation of nanoporous diopside bioglass (nDPB) into gliadin (GL) matrix, macro–nanoporous scaffolds of nDPB/GL composites (DGC) were fabricated by method of solution compressing and particles leaching. Results: The results revealed that the DGC scaffolds possessed well-interconnected macropores of 200–500 µm and nanopores of 4 nm, and the porosity and degradability of DGC scaffolds remarkably increased with the increase in nDPB content. In addition, in vitro cell experiments revealed that the adhesion and growth of MC3T3-E1 cells on DGC scaffolds were significantly promoted, which depended on nDPB content. Moreover, the results of histological evaluations confirmed that the osteogenic properties and degradability of DGC scaffolds in vivo significantly improved, which were nDPB content dependent. Furthermore, the results of immunohistochemical analysis demonstrated that, with the increase in nDPB content, the type I collagen expression in DGC scaffolds in vivo obviously enhanced, indicating excellent osteogenesis. Discussion and conclusion: The results demonstrated that the DGC scaffolds containing 30 wt% nDPB (30nDGC) exhibited good biocompatibility and new bone formation ability, which might have a great potential for applications in bone regeneration. Keywords: nanoporous diopside bioglass, gliadin, macro-nanoporous, MC3T3-E1, osteogenesis
format	article
author	Ba Z Chen Z Huang Y Feng D Zhao Q Zhu J Wu D
author_facet	Ba Z Chen Z Huang Y Feng D Zhao Q Zhu J Wu D
author_sort	Ba Z
title	Nanoporous diopside modulates biocompatibility, degradability and osteogenesis of bioactive scaffolds of gliadin-based composites for new bone formation
title_short	Nanoporous diopside modulates biocompatibility, degradability and osteogenesis of bioactive scaffolds of gliadin-based composites for new bone formation
title_full	Nanoporous diopside modulates biocompatibility, degradability and osteogenesis of bioactive scaffolds of gliadin-based composites for new bone formation
title_fullStr	Nanoporous diopside modulates biocompatibility, degradability and osteogenesis of bioactive scaffolds of gliadin-based composites for new bone formation
title_full_unstemmed	Nanoporous diopside modulates biocompatibility, degradability and osteogenesis of bioactive scaffolds of gliadin-based composites for new bone formation
title_sort	nanoporous diopside modulates biocompatibility, degradability and osteogenesis of bioactive scaffolds of gliadin-based composites for new bone formation
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/f624134a4cd0441d9f913b642a8234bf
work_keys_str_mv	AT baz nanoporousdiopsidemodulatesbiocompatibilitydegradabilityandosteogenesisofbioactivescaffoldsofgliadinbasedcompositesfornewboneformation AT chenz nanoporousdiopsidemodulatesbiocompatibilitydegradabilityandosteogenesisofbioactivescaffoldsofgliadinbasedcompositesfornewboneformation AT huangy nanoporousdiopsidemodulatesbiocompatibilitydegradabilityandosteogenesisofbioactivescaffoldsofgliadinbasedcompositesfornewboneformation AT fengd nanoporousdiopsidemodulatesbiocompatibilitydegradabilityandosteogenesisofbioactivescaffoldsofgliadinbasedcompositesfornewboneformation AT zhaoq nanoporousdiopsidemodulatesbiocompatibilitydegradabilityandosteogenesisofbioactivescaffoldsofgliadinbasedcompositesfornewboneformation AT zhuj nanoporousdiopsidemodulatesbiocompatibilitydegradabilityandosteogenesisofbioactivescaffoldsofgliadinbasedcompositesfornewboneformation AT wud nanoporousdiopsidemodulatesbiocompatibilitydegradabilityandosteogenesisofbioactivescaffoldsofgliadinbasedcompositesfornewboneformation
_version_	1718400748242337792

Nanoporous diopside modulates biocompatibility, degradability and osteogenesis of bioactive scaffolds of gliadin-based composites for new bone formation

Ejemplares similares