Improvements in Smartphone and Night Vision Imaging Technologies Enable Low Cost, On-Site Assays of Bioluminescent Cells

Technologies enabling on-site environmental detection or medical diagnostics in resource-limited settings have a strong disruptive potential compared to current analytical approaches that require trained personnel in laboratories with immobile, resource intensive instrumentation. Handheld devices, s...

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Autores principales:	Mark Wienhold, Andrew Kirkpatrick, Tingting Xu, Steven Ripp, Gary Sayler, Dan Close
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	bioluminescence luciferase optical imaging remote sensing smartphone portable analytical device Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/f62a2c4fa14f46159732a7c6fb641d49
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spelling	oai:doaj.org-article:f62a2c4fa14f46159732a7c6fb641d492021-11-19T07:18:02ZImprovements in Smartphone and Night Vision Imaging Technologies Enable Low Cost, On-Site Assays of Bioluminescent Cells2296-418510.3389/fbioe.2021.767313https://doaj.org/article/f62a2c4fa14f46159732a7c6fb641d492021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.767313/fullhttps://doaj.org/toc/2296-4185Technologies enabling on-site environmental detection or medical diagnostics in resource-limited settings have a strong disruptive potential compared to current analytical approaches that require trained personnel in laboratories with immobile, resource intensive instrumentation. Handheld devices, such as smartphones, are now routinely produced with CPUs, RAM, wireless data transfer capabilities, and high-resolution complementary metal oxide semiconductor (CMOS) cameras capable of supporting the capture and processing of bioluminescent signals. In theory, combining the capabilities of these devices with continuously bioluminescent human cell-based bioreporters would allow them to replicate the functionality of more expensive, more complex, and less flexible platforms while supporting human-relevant conclusions. In this work, we compare the performance of smartphone (CMOS) and night vision (image intensifier) devices with in vivo (CCD camera), and in vitro (photomultiplier tube) laboratory instrumentation for monitoring signal dynamics from continuously bioluminescent human cellular models under toxic, stable, and induced expression scenarios. All systems detected bioluminescence from cells at common plating densities. While the in vivo and in vitro systems were more sensitive and detected signal dynamics representing cellular health changes earlier, the night vision and smartphone systems also detected these changes with relatively similar coefficients of variation and linear detection capabilities. The smartphone system did not detect transcriptional induction. The night vision system did detect transcriptional activation, but was less sensitive than the in vivo or in vitro systems and required a stronger induction before the change could be resolved.Mark WienholdAndrew KirkpatrickTingting XuSteven RippSteven RippGary SaylerDan CloseFrontiers Media S.A.articlebioluminescenceluciferaseoptical imagingremote sensingsmartphoneportable analytical deviceBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioluminescence luciferase optical imaging remote sensing smartphone portable analytical device Biotechnology TP248.13-248.65
spellingShingle	bioluminescence luciferase optical imaging remote sensing smartphone portable analytical device Biotechnology TP248.13-248.65 Mark Wienhold Andrew Kirkpatrick Tingting Xu Steven Ripp Steven Ripp Gary Sayler Dan Close Improvements in Smartphone and Night Vision Imaging Technologies Enable Low Cost, On-Site Assays of Bioluminescent Cells
description	Technologies enabling on-site environmental detection or medical diagnostics in resource-limited settings have a strong disruptive potential compared to current analytical approaches that require trained personnel in laboratories with immobile, resource intensive instrumentation. Handheld devices, such as smartphones, are now routinely produced with CPUs, RAM, wireless data transfer capabilities, and high-resolution complementary metal oxide semiconductor (CMOS) cameras capable of supporting the capture and processing of bioluminescent signals. In theory, combining the capabilities of these devices with continuously bioluminescent human cell-based bioreporters would allow them to replicate the functionality of more expensive, more complex, and less flexible platforms while supporting human-relevant conclusions. In this work, we compare the performance of smartphone (CMOS) and night vision (image intensifier) devices with in vivo (CCD camera), and in vitro (photomultiplier tube) laboratory instrumentation for monitoring signal dynamics from continuously bioluminescent human cellular models under toxic, stable, and induced expression scenarios. All systems detected bioluminescence from cells at common plating densities. While the in vivo and in vitro systems were more sensitive and detected signal dynamics representing cellular health changes earlier, the night vision and smartphone systems also detected these changes with relatively similar coefficients of variation and linear detection capabilities. The smartphone system did not detect transcriptional induction. The night vision system did detect transcriptional activation, but was less sensitive than the in vivo or in vitro systems and required a stronger induction before the change could be resolved.
format	article
author	Mark Wienhold Andrew Kirkpatrick Tingting Xu Steven Ripp Steven Ripp Gary Sayler Dan Close
author_facet	Mark Wienhold Andrew Kirkpatrick Tingting Xu Steven Ripp Steven Ripp Gary Sayler Dan Close
author_sort	Mark Wienhold
title	Improvements in Smartphone and Night Vision Imaging Technologies Enable Low Cost, On-Site Assays of Bioluminescent Cells
title_short	Improvements in Smartphone and Night Vision Imaging Technologies Enable Low Cost, On-Site Assays of Bioluminescent Cells
title_full	Improvements in Smartphone and Night Vision Imaging Technologies Enable Low Cost, On-Site Assays of Bioluminescent Cells
title_fullStr	Improvements in Smartphone and Night Vision Imaging Technologies Enable Low Cost, On-Site Assays of Bioluminescent Cells
title_full_unstemmed	Improvements in Smartphone and Night Vision Imaging Technologies Enable Low Cost, On-Site Assays of Bioluminescent Cells
title_sort	improvements in smartphone and night vision imaging technologies enable low cost, on-site assays of bioluminescent cells
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f62a2c4fa14f46159732a7c6fb641d49
work_keys_str_mv	AT markwienhold improvementsinsmartphoneandnightvisionimagingtechnologiesenablelowcostonsiteassaysofbioluminescentcells AT andrewkirkpatrick improvementsinsmartphoneandnightvisionimagingtechnologiesenablelowcostonsiteassaysofbioluminescentcells AT tingtingxu improvementsinsmartphoneandnightvisionimagingtechnologiesenablelowcostonsiteassaysofbioluminescentcells AT stevenripp improvementsinsmartphoneandnightvisionimagingtechnologiesenablelowcostonsiteassaysofbioluminescentcells AT stevenripp improvementsinsmartphoneandnightvisionimagingtechnologiesenablelowcostonsiteassaysofbioluminescentcells AT garysayler improvementsinsmartphoneandnightvisionimagingtechnologiesenablelowcostonsiteassaysofbioluminescentcells AT danclose improvementsinsmartphoneandnightvisionimagingtechnologiesenablelowcostonsiteassaysofbioluminescentcells
_version_	1718420351639093248

Improvements in Smartphone and Night Vision Imaging Technologies Enable Low Cost, On-Site Assays of Bioluminescent Cells

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