YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis.

MIA/CD-RAP is a small, secreted protein involved in cartilage differentiation and melanoma progression. We recently revealed that p54(nrb) acts as a mediator of MIA/CD-RAP action to promote chondrogenesis and the progression of malignant melanoma. As the molecular mechanism of MIA/CD-RAP action in c...

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Autores principales:	Rainer Schmid, Katharina Meyer, Rainer Spang, Birgit Schittek, Anja Katrin Bosserhoff
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f62ec8949d7f415dac0b05d36361bac0
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spelling	oai:doaj.org-article:f62ec8949d7f415dac0b05d36361bac02021-11-18T08:42:15ZYBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis.1932-620310.1371/journal.pone.0082166https://doaj.org/article/f62ec8949d7f415dac0b05d36361bac02013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349210/?tool=EBIhttps://doaj.org/toc/1932-6203MIA/CD-RAP is a small, secreted protein involved in cartilage differentiation and melanoma progression. We recently revealed that p54(nrb) acts as a mediator of MIA/CD-RAP action to promote chondrogenesis and the progression of malignant melanoma. As the molecular mechanism of MIA/CD-RAP action in cartilage has not been defined in detail until now, we aimed to understand the regulation of p54(nrb) transcription in chondrogenesis. We concentrated on the previously described MIA/CD-RAP-dependent regulatory region in the p54(nrb) promoter and characterized the transcriptional regulation of p54(nrb) by MIA/CD-RAP in cartilage. A series of truncated p54(nrb) promoter constructs and mutagenesis analysis revealed that the transcription factor YBX1, which has not been investigated in chondrogenesis thus far, is the mediator of MIA/CD-RAP dependent activation of p54(nrb) transcription. A systematic analysis of genes carrying this binding site in their promoter region revealed further potential MIA/CD-RAP-regulated genes that have been implicated in cartilage differentiation. In summary, we described the effects of MIA/CD-RAP on transcriptional regulation in chondrocytes. Understanding the regulation of p54(nrb) via YBX1 contributes to the understanding of chondrogenesis. Uncovering new downstream effectors that function via the activation of YBX1 supports the important role of MIA/CD-RAP in these processes.Rainer SchmidKatharina MeyerRainer SpangBirgit SchittekAnja Katrin BosserhoffPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82166 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rainer Schmid Katharina Meyer Rainer Spang Birgit Schittek Anja Katrin Bosserhoff YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis.
description	MIA/CD-RAP is a small, secreted protein involved in cartilage differentiation and melanoma progression. We recently revealed that p54(nrb) acts as a mediator of MIA/CD-RAP action to promote chondrogenesis and the progression of malignant melanoma. As the molecular mechanism of MIA/CD-RAP action in cartilage has not been defined in detail until now, we aimed to understand the regulation of p54(nrb) transcription in chondrogenesis. We concentrated on the previously described MIA/CD-RAP-dependent regulatory region in the p54(nrb) promoter and characterized the transcriptional regulation of p54(nrb) by MIA/CD-RAP in cartilage. A series of truncated p54(nrb) promoter constructs and mutagenesis analysis revealed that the transcription factor YBX1, which has not been investigated in chondrogenesis thus far, is the mediator of MIA/CD-RAP dependent activation of p54(nrb) transcription. A systematic analysis of genes carrying this binding site in their promoter region revealed further potential MIA/CD-RAP-regulated genes that have been implicated in cartilage differentiation. In summary, we described the effects of MIA/CD-RAP on transcriptional regulation in chondrocytes. Understanding the regulation of p54(nrb) via YBX1 contributes to the understanding of chondrogenesis. Uncovering new downstream effectors that function via the activation of YBX1 supports the important role of MIA/CD-RAP in these processes.
format	article
author	Rainer Schmid Katharina Meyer Rainer Spang Birgit Schittek Anja Katrin Bosserhoff
author_facet	Rainer Schmid Katharina Meyer Rainer Spang Birgit Schittek Anja Katrin Bosserhoff
author_sort	Rainer Schmid
title	YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis.
title_short	YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis.
title_full	YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis.
title_fullStr	YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis.
title_full_unstemmed	YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis.
title_sort	ybx1 is a modulator of mia/cd-rap-dependent chondrogenesis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f62ec8949d7f415dac0b05d36361bac0
work_keys_str_mv	AT rainerschmid ybx1isamodulatorofmiacdrapdependentchondrogenesis AT katharinameyer ybx1isamodulatorofmiacdrapdependentchondrogenesis AT rainerspang ybx1isamodulatorofmiacdrapdependentchondrogenesis AT birgitschittek ybx1isamodulatorofmiacdrapdependentchondrogenesis AT anjakatrinbosserhoff ybx1isamodulatorofmiacdrapdependentchondrogenesis
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YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis.

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