Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats

Xiuying Li,1,* Zhenhong Wei,1,* Wei Zhang,2 Huiying Lv,1 Jing Li,1 Liya Wu,1 Hao Zhang,3 Bai Yang,3 Mingji Zhu,4 Jinlan Jiang1 1Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, Jilin, People’s Republic of China; 2Department of Radiotherapy, The Second...

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Autores principales:	Li X, Wei Z, Zhang W, Lv H, Li J, Wu L, Zhang H, Yang B, Zhu M, Jiang J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	mesenchymal stem cells skin injury magnetic targeting fe3o4 nanoparticle anti-inflammatory effect Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f631a5e66d694e98821629d39453bb7b
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spelling	oai:doaj.org-article:f631a5e66d694e98821629d39453bb7b2021-12-02T08:28:38ZAnti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats1178-2013https://doaj.org/article/f631a5e66d694e98821629d39453bb7b2020-08-01T00:00:00Zhttps://www.dovepress.com/anti-inflammatory-effects-of-magnetically-targeted-mesenchymal-stem-ce-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xiuying Li,1,* Zhenhong Wei,1,* Wei Zhang,2 Huiying Lv,1 Jing Li,1 Liya Wu,1 Hao Zhang,3 Bai Yang,3 Mingji Zhu,4 Jinlan Jiang1 1Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, Jilin, People’s Republic of China; 2Department of Radiotherapy, The Second Hospital of Jilin University, Changchun, Jilin, People’s Republic of China; 3State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, Jilin, People’s Republic of China; 4Dermatological Department, China-Japan Union Hospital of Jilin University, Changchun, Jilin, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jinlan JiangScientific Research Center, China-Japan Union Hospital of Jilin University, No. 126 Xiantai Street, Changchun 130033, Jilin, People’s Republic of ChinaEmail jiangjinlan@jlu.edu.cnMingji Zhu Dermatological Department No. 126 Xiantai Street, Changchun 130033, Jilin, People’s Republic of ChinaTel/Fax +86 04 318 499 5423Email zmj@jlu.edu.cnIntroduction: Mesenchymal stem cells (MSCs) are a promising resource for tissue regeneration and repair. However, their clinical application is hindered by technical limitations related to MSC enrichment at the target sites.Methods: MSCs were labeled with magnetic Fe3O4 nanoparticles (NPs). We analyzed the effects of NP on cell proliferation, stem cell characteristics, and cytokine secretion. Furthermore, we induced NP-labeled MSC migration with an external magnetic field toward laser-induced skin wounds in rats and evaluated the associated anti-inflammatory effects.Results: Fe3O4 NP application did not adversely affect MSC characteristics. Moreover, Fe3O4 NP-labeled MSCs presented increased anti-inflammatory cytokine and chemokine production compared with unlabeled MSCs. Furthermore, MSCs accumulated at the injury site and magnetic targeting promoted NP-labeled MSC migration toward burn injury sites in vivo. On day 7 following MSC injection, reduced inflammation and promoted angiogenesis were observed in the magnetically targeted MSC group. In addition, anti-inflammatory factors were upregulated, whereas pro-inflammatory factors were downregulated within the magnetically targeted MSC group compared with those in the PBS group.Conclusion: This study demonstrates that magnetically targeted MSCs contribute to cell migration to the site of skin injury, improve anti-inflammatory effects and enhance angiogenesis compared with MSC injection alone. Therefore, magnetically targeted MSC therapy may be an effective treatment approach for epithelial tissue injuries.Keywords: mesenchymal stem cells, skin injury, magnetic targeting, Fe3O4 nanoparticle, anti-inflammatory effectLi XWei ZZhang WLv HLi JWu LZhang HYang BZhu MJiang JDove Medical Pressarticlemesenchymal stem cellsskin injurymagnetic targetingfe3o4 nanoparticleanti-inflammatory effectMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 5645-5659 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	mesenchymal stem cells skin injury magnetic targeting fe3o4 nanoparticle anti-inflammatory effect Medicine (General) R5-920
spellingShingle	mesenchymal stem cells skin injury magnetic targeting fe3o4 nanoparticle anti-inflammatory effect Medicine (General) R5-920 Li X Wei Z Zhang W Lv H Li J Wu L Zhang H Yang B Zhu M Jiang J Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats
description	Xiuying Li,1,* Zhenhong Wei,1,* Wei Zhang,2 Huiying Lv,1 Jing Li,1 Liya Wu,1 Hao Zhang,3 Bai Yang,3 Mingji Zhu,4 Jinlan Jiang1 1Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, Jilin, People’s Republic of China; 2Department of Radiotherapy, The Second Hospital of Jilin University, Changchun, Jilin, People’s Republic of China; 3State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, Jilin, People’s Republic of China; 4Dermatological Department, China-Japan Union Hospital of Jilin University, Changchun, Jilin, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jinlan JiangScientific Research Center, China-Japan Union Hospital of Jilin University, No. 126 Xiantai Street, Changchun 130033, Jilin, People’s Republic of ChinaEmail jiangjinlan@jlu.edu.cnMingji Zhu Dermatological Department No. 126 Xiantai Street, Changchun 130033, Jilin, People’s Republic of ChinaTel/Fax +86 04 318 499 5423Email zmj@jlu.edu.cnIntroduction: Mesenchymal stem cells (MSCs) are a promising resource for tissue regeneration and repair. However, their clinical application is hindered by technical limitations related to MSC enrichment at the target sites.Methods: MSCs were labeled with magnetic Fe3O4 nanoparticles (NPs). We analyzed the effects of NP on cell proliferation, stem cell characteristics, and cytokine secretion. Furthermore, we induced NP-labeled MSC migration with an external magnetic field toward laser-induced skin wounds in rats and evaluated the associated anti-inflammatory effects.Results: Fe3O4 NP application did not adversely affect MSC characteristics. Moreover, Fe3O4 NP-labeled MSCs presented increased anti-inflammatory cytokine and chemokine production compared with unlabeled MSCs. Furthermore, MSCs accumulated at the injury site and magnetic targeting promoted NP-labeled MSC migration toward burn injury sites in vivo. On day 7 following MSC injection, reduced inflammation and promoted angiogenesis were observed in the magnetically targeted MSC group. In addition, anti-inflammatory factors were upregulated, whereas pro-inflammatory factors were downregulated within the magnetically targeted MSC group compared with those in the PBS group.Conclusion: This study demonstrates that magnetically targeted MSCs contribute to cell migration to the site of skin injury, improve anti-inflammatory effects and enhance angiogenesis compared with MSC injection alone. Therefore, magnetically targeted MSC therapy may be an effective treatment approach for epithelial tissue injuries.Keywords: mesenchymal stem cells, skin injury, magnetic targeting, Fe3O4 nanoparticle, anti-inflammatory effect
format	article
author	Li X Wei Z Zhang W Lv H Li J Wu L Zhang H Yang B Zhu M Jiang J
author_facet	Li X Wei Z Zhang W Lv H Li J Wu L Zhang H Yang B Zhu M Jiang J
author_sort	Li X
title	Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats
title_short	Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats
title_full	Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats
title_fullStr	Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats
title_full_unstemmed	Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats
title_sort	anti-inflammatory effects of magnetically targeted mesenchymal stem cells on laser-induced skin injuries in rats
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/f631a5e66d694e98821629d39453bb7b
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Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats

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