Functional genomics study of Pseudomonas putida to determine traits associated with avoidance of a myxobacterial predator

Abstract Predation contributes to the structure and diversity of microbial communities. Predatory myxobacteria are ubiquitous to a variety of microbial habitats and capably consume a broad diversity of microbial prey. Predator–prey experiments utilizing myxobacteria have provided details into predat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shukria Akbar, D. Cole Stevens
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f633ef6cfe7144aaa4549047e2fa6a0f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f633ef6cfe7144aaa4549047e2fa6a0f
record_format	dspace
spelling	oai:doaj.org-article:f633ef6cfe7144aaa4549047e2fa6a0f2021-12-02T16:27:45ZFunctional genomics study of Pseudomonas putida to determine traits associated with avoidance of a myxobacterial predator10.1038/s41598-021-96046-82045-2322https://doaj.org/article/f633ef6cfe7144aaa4549047e2fa6a0f2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96046-8https://doaj.org/toc/2045-2322Abstract Predation contributes to the structure and diversity of microbial communities. Predatory myxobacteria are ubiquitous to a variety of microbial habitats and capably consume a broad diversity of microbial prey. Predator–prey experiments utilizing myxobacteria have provided details into predatory mechanisms and features that facilitate consumption of prey. However, prey resistance to myxobacterial predation remains underexplored, and prey resistances have been observed exclusively from predator–prey experiments that included the model myxobacterium Myxococcus xanthus. Utilizing a predator–prey pairing that instead included the myxobacterium, Cystobacter ferrugineus, with Pseudomonas putida as prey, we observed surviving phenotypes capable of eluding predation. Comparative transcriptomics between P. putida unexposed to C. ferrugineus and the survivor phenotype suggested that increased expression of efflux pumps, genes associated with mucoid conversion, and various membrane features contribute to predator avoidance. Unique features observed from the survivor phenotype when compared to the parent P. putida include small colony variation, efflux-mediated antibiotic resistance, phenazine-1-carboxylic acid production, and increased mucoid conversion. These results demonstrate the utility of myxobacterial predator–prey models and provide insight into prey resistances in response to predatory stress that might contribute to the phenotypic diversity and structure of bacterial communities.Shukria AkbarD. Cole StevensNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shukria Akbar D. Cole Stevens Functional genomics study of Pseudomonas putida to determine traits associated with avoidance of a myxobacterial predator
description	Abstract Predation contributes to the structure and diversity of microbial communities. Predatory myxobacteria are ubiquitous to a variety of microbial habitats and capably consume a broad diversity of microbial prey. Predator–prey experiments utilizing myxobacteria have provided details into predatory mechanisms and features that facilitate consumption of prey. However, prey resistance to myxobacterial predation remains underexplored, and prey resistances have been observed exclusively from predator–prey experiments that included the model myxobacterium Myxococcus xanthus. Utilizing a predator–prey pairing that instead included the myxobacterium, Cystobacter ferrugineus, with Pseudomonas putida as prey, we observed surviving phenotypes capable of eluding predation. Comparative transcriptomics between P. putida unexposed to C. ferrugineus and the survivor phenotype suggested that increased expression of efflux pumps, genes associated with mucoid conversion, and various membrane features contribute to predator avoidance. Unique features observed from the survivor phenotype when compared to the parent P. putida include small colony variation, efflux-mediated antibiotic resistance, phenazine-1-carboxylic acid production, and increased mucoid conversion. These results demonstrate the utility of myxobacterial predator–prey models and provide insight into prey resistances in response to predatory stress that might contribute to the phenotypic diversity and structure of bacterial communities.
format	article
author	Shukria Akbar D. Cole Stevens
author_facet	Shukria Akbar D. Cole Stevens
author_sort	Shukria Akbar
title	Functional genomics study of Pseudomonas putida to determine traits associated with avoidance of a myxobacterial predator
title_short	Functional genomics study of Pseudomonas putida to determine traits associated with avoidance of a myxobacterial predator
title_full	Functional genomics study of Pseudomonas putida to determine traits associated with avoidance of a myxobacterial predator
title_fullStr	Functional genomics study of Pseudomonas putida to determine traits associated with avoidance of a myxobacterial predator
title_full_unstemmed	Functional genomics study of Pseudomonas putida to determine traits associated with avoidance of a myxobacterial predator
title_sort	functional genomics study of pseudomonas putida to determine traits associated with avoidance of a myxobacterial predator
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f633ef6cfe7144aaa4549047e2fa6a0f
work_keys_str_mv	AT shukriaakbar functionalgenomicsstudyofpseudomonasputidatodeterminetraitsassociatedwithavoidanceofamyxobacterialpredator AT dcolestevens functionalgenomicsstudyofpseudomonasputidatodeterminetraitsassociatedwithavoidanceofamyxobacterialpredator
_version_	1718384029101719552

Functional genomics study of Pseudomonas putida to determine traits associated with avoidance of a myxobacterial predator

Ejemplares similares