Comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes.

<h4>Background</h4>Proliferative vitreoretinopathy (PVR) is a destructive complication of retinal detachment and vitreoretinal surgery which can lead to severe vision reduction by tractional retinal detachments. The purpose of this study was to determine the gene expression profile of ep...

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Autores principales:	Ryo Asato, Shigeo Yoshida, Atsushi Ogura, Takahito Nakama, Keijiro Ishikawa, Shintaro Nakao, Yukio Sassa, Hiroshi Enaida, Yuji Oshima, Kazuho Ikeo, Takashi Gojobori, Toshihiro Kono, Tatsuro Ishibashi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f6354fee8896493293be61513b907dc7
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spelling	oai:doaj.org-article:f6354fee8896493293be61513b907dc72021-11-18T08:00:20ZComparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes.1932-620310.1371/journal.pone.0054191https://doaj.org/article/f6354fee8896493293be61513b907dc72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23372684/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Proliferative vitreoretinopathy (PVR) is a destructive complication of retinal detachment and vitreoretinal surgery which can lead to severe vision reduction by tractional retinal detachments. The purpose of this study was to determine the gene expression profile of epiretinal membranes (ERMs) associated with a PVR (PVR-ERM) and to compare it to the expression profile of less-aggressive secondary ERMs.<h4>Methodology/principal findings</h4>A PCR-amplified complementary DNA (cDNA) library was constructed using the RNAs isolated from ERMs obtained during vitrectomy. The sequence from the 5' end was obtained for randomly selected clones and used to generate expressed sequence tags (ESTs). We obtained 1116 nonredundant clusters representing individual genes expressed in PVR-ERMs, and 799 clusters representing the genes expressed in secondary ERMs. The transcriptome of the PVR-ERMs was subdivided by functional subsets of genes related to metabolism, cell adhesion, cytoskeleton, signaling, and other functions, by FatiGo analysis. The genes highly expressed in PVR-ERMs were compared to those expressed in the secondary ERMs, and these were subdivided by cell adhesion, proliferation, and other functions. Querying 10 cell adhesion-related genes against the STRING database yielded 70 possible physical relationships to other genes/proteins, which included an additional 60 genes that were not detected in the PVR-ERM library. Of these, soluble CD44 and soluble vascular cellular adhesion molecule-1 were significantly increased in the vitreous of patients with PVR.<h4>Conclusions/significance</h4>Our results support an earlier hypothesis that a PVR-ERM, even from genomic points of view, is an aberrant form of wound healing response. Genes preferentially expressed in PVR-ERMs may play an important role in the progression of PVR and could be served as therapeutic targets.Ryo AsatoShigeo YoshidaAtsushi OguraTakahito NakamaKeijiro IshikawaShintaro NakaoYukio SassaHiroshi EnaidaYuji OshimaKazuho IkeoTakashi GojoboriToshihiro KonoTatsuro IshibashiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e54191 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryo Asato Shigeo Yoshida Atsushi Ogura Takahito Nakama Keijiro Ishikawa Shintaro Nakao Yukio Sassa Hiroshi Enaida Yuji Oshima Kazuho Ikeo Takashi Gojobori Toshihiro Kono Tatsuro Ishibashi Comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes.
description	<h4>Background</h4>Proliferative vitreoretinopathy (PVR) is a destructive complication of retinal detachment and vitreoretinal surgery which can lead to severe vision reduction by tractional retinal detachments. The purpose of this study was to determine the gene expression profile of epiretinal membranes (ERMs) associated with a PVR (PVR-ERM) and to compare it to the expression profile of less-aggressive secondary ERMs.<h4>Methodology/principal findings</h4>A PCR-amplified complementary DNA (cDNA) library was constructed using the RNAs isolated from ERMs obtained during vitrectomy. The sequence from the 5' end was obtained for randomly selected clones and used to generate expressed sequence tags (ESTs). We obtained 1116 nonredundant clusters representing individual genes expressed in PVR-ERMs, and 799 clusters representing the genes expressed in secondary ERMs. The transcriptome of the PVR-ERMs was subdivided by functional subsets of genes related to metabolism, cell adhesion, cytoskeleton, signaling, and other functions, by FatiGo analysis. The genes highly expressed in PVR-ERMs were compared to those expressed in the secondary ERMs, and these were subdivided by cell adhesion, proliferation, and other functions. Querying 10 cell adhesion-related genes against the STRING database yielded 70 possible physical relationships to other genes/proteins, which included an additional 60 genes that were not detected in the PVR-ERM library. Of these, soluble CD44 and soluble vascular cellular adhesion molecule-1 were significantly increased in the vitreous of patients with PVR.<h4>Conclusions/significance</h4>Our results support an earlier hypothesis that a PVR-ERM, even from genomic points of view, is an aberrant form of wound healing response. Genes preferentially expressed in PVR-ERMs may play an important role in the progression of PVR and could be served as therapeutic targets.
format	article
author	Ryo Asato Shigeo Yoshida Atsushi Ogura Takahito Nakama Keijiro Ishikawa Shintaro Nakao Yukio Sassa Hiroshi Enaida Yuji Oshima Kazuho Ikeo Takashi Gojobori Toshihiro Kono Tatsuro Ishibashi
author_facet	Ryo Asato Shigeo Yoshida Atsushi Ogura Takahito Nakama Keijiro Ishikawa Shintaro Nakao Yukio Sassa Hiroshi Enaida Yuji Oshima Kazuho Ikeo Takashi Gojobori Toshihiro Kono Tatsuro Ishibashi
author_sort	Ryo Asato
title	Comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes.
title_short	Comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes.
title_full	Comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes.
title_fullStr	Comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes.
title_full_unstemmed	Comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes.
title_sort	comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f6354fee8896493293be61513b907dc7
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Comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes.

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