High‐resolution HLA genotyping identifies alleles associated with severe COVID‐19: A preliminary study from India

Abstract Introduction Human leukocyte antigen (HLA) variability has been demonstrated to be associated with susceptibility/severity of COVID‐19. High‐resolution HLA genotyping to identify alleles associated with severe COVID‐19 in an Indian cohort was performed. Methods Quantitative reverse‐transcri...

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Autores principales:	Ravikanth Vishnubhotla, Mitnala Sasikala, Vijayasarathy Ketavarapu, Duvvur Nageshwar Reddy
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	COVID‐19 HLA genotyping SARS‐CoV‐2 severe COVID‐19 vaccination Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/f638b4984c9e4c378a3aa19c859cf345
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spelling	oai:doaj.org-article:f638b4984c9e4c378a3aa19c859cf3452021-11-12T19:57:15ZHigh‐resolution HLA genotyping identifies alleles associated with severe COVID‐19: A preliminary study from India2050-452710.1002/iid3.481https://doaj.org/article/f638b4984c9e4c378a3aa19c859cf3452021-12-01T00:00:00Zhttps://doi.org/10.1002/iid3.481https://doaj.org/toc/2050-4527Abstract Introduction Human leukocyte antigen (HLA) variability has been demonstrated to be associated with susceptibility/severity of COVID‐19. High‐resolution HLA genotyping to identify alleles associated with severe COVID‐19 in an Indian cohort was performed. Methods Quantitative reverse‐transcription polymerase chain reaction‐confirmed SARS‐CoV‐2‐positive patients with mild/moderate/severe disease (n = 54) and asymptomatic (n = 42) were recruited and genotyped for 11‐HLA loci on MiSeq using NGSgo®‐MX11‐3 and analyzed (NGSengine; GenDx). Results A significant difference in alleles between the groups was identified for HLA‐C04:01:01:01, HLA‐DRB501:01:01:02, HLA‐DQA103:01:01:01, HLA‐DPB104:01:01:41, and HLA‐DPA101:03:01:02. Alleles namely, HLA‐C04:01:01:01 (OR: 5.71; 95% CI: 1.2–27.14; p = .02), HLA‐DRB501:01:01:02 (OR: 2.94; 95% CI: 1.1–7.84; p = .03), DQA103:01:01:01 (OR: 22.47; 95% CI: 1.28–393.5; p = .03), HLA‐DPB104:01:01:41 (OR: 9.44; 95% CI: 0.5–175.81; p = .13), and HLA‐DPA101:03:01:02 (OR: 8.27; 95% CI: 2.26–30.21; p = .001) were associated with severe COVID‐19. Conclusion Genotyping for these alleles will enable identification of individuals at risk of severe disease and stratification for preferential vaccination.Ravikanth VishnubhotlaMitnala SasikalaVijayasarathy KetavarapuDuvvur Nageshwar ReddyWileyarticleCOVID‐19HLA genotypingSARS‐CoV‐2severe COVID‐19vaccinationImmunologic diseases. AllergyRC581-607ENImmunity, Inflammation and Disease, Vol 9, Iss 4, Pp 1781-1785 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	COVID‐19 HLA genotyping SARS‐CoV‐2 severe COVID‐19 vaccination Immunologic diseases. Allergy RC581-607
spellingShingle	COVID‐19 HLA genotyping SARS‐CoV‐2 severe COVID‐19 vaccination Immunologic diseases. Allergy RC581-607 Ravikanth Vishnubhotla Mitnala Sasikala Vijayasarathy Ketavarapu Duvvur Nageshwar Reddy High‐resolution HLA genotyping identifies alleles associated with severe COVID‐19: A preliminary study from India
description	Abstract Introduction Human leukocyte antigen (HLA) variability has been demonstrated to be associated with susceptibility/severity of COVID‐19. High‐resolution HLA genotyping to identify alleles associated with severe COVID‐19 in an Indian cohort was performed. Methods Quantitative reverse‐transcription polymerase chain reaction‐confirmed SARS‐CoV‐2‐positive patients with mild/moderate/severe disease (n = 54) and asymptomatic (n = 42) were recruited and genotyped for 11‐HLA loci on MiSeq using NGSgo®‐MX11‐3 and analyzed (NGSengine; GenDx). Results A significant difference in alleles between the groups was identified for HLA‐C04:01:01:01, HLA‐DRB501:01:01:02, HLA‐DQA103:01:01:01, HLA‐DPB104:01:01:41, and HLA‐DPA101:03:01:02. Alleles namely, HLA‐C04:01:01:01 (OR: 5.71; 95% CI: 1.2–27.14; p = .02), HLA‐DRB501:01:01:02 (OR: 2.94; 95% CI: 1.1–7.84; p = .03), DQA103:01:01:01 (OR: 22.47; 95% CI: 1.28–393.5; p = .03), HLA‐DPB104:01:01:41 (OR: 9.44; 95% CI: 0.5–175.81; p = .13), and HLA‐DPA101:03:01:02 (OR: 8.27; 95% CI: 2.26–30.21; p = .001) were associated with severe COVID‐19. Conclusion Genotyping for these alleles will enable identification of individuals at risk of severe disease and stratification for preferential vaccination.
format	article
author	Ravikanth Vishnubhotla Mitnala Sasikala Vijayasarathy Ketavarapu Duvvur Nageshwar Reddy
author_facet	Ravikanth Vishnubhotla Mitnala Sasikala Vijayasarathy Ketavarapu Duvvur Nageshwar Reddy
author_sort	Ravikanth Vishnubhotla
title	High‐resolution HLA genotyping identifies alleles associated with severe COVID‐19: A preliminary study from India
title_short	High‐resolution HLA genotyping identifies alleles associated with severe COVID‐19: A preliminary study from India
title_full	High‐resolution HLA genotyping identifies alleles associated with severe COVID‐19: A preliminary study from India
title_fullStr	High‐resolution HLA genotyping identifies alleles associated with severe COVID‐19: A preliminary study from India
title_full_unstemmed	High‐resolution HLA genotyping identifies alleles associated with severe COVID‐19: A preliminary study from India
title_sort	high‐resolution hla genotyping identifies alleles associated with severe covid‐19: a preliminary study from india
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/f638b4984c9e4c378a3aa19c859cf345
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High‐resolution HLA genotyping identifies alleles associated with severe COVID‐19: A preliminary study from India

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