Experiment on Inhibiting NEK7 to Promote Apoptosis of Hepatocellular Carcinoma Cells

Objective To use in vitro experiments to verify the changes of proliferation, senescence and apoptosis of hepatocellular carcinoma cells after inhibiting the expression of NEK7, and to explore the related molecular mechanism. Methods Western blot and RT-PCR were used to detect the expression of NEK7...

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Autores principales:	SONG Yanzhou, ZHANG Kun, CHEN Qijun, WEI Wenping, ZHAO Xin, LI Zhiwei, LI Wei
Formato:	article
Lenguaje:	ZH
Publicado:	Magazine House of Cancer Research on Prevention and Treatment 2021
Materias:	hepatocellular carcinoma nek7 cell cycle Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/f6393d777b2d4b9da477229f535e9fef
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spelling	oai:doaj.org-article:f6393d777b2d4b9da477229f535e9fef2021-11-05T02:22:52ZExperiment on Inhibiting NEK7 to Promote Apoptosis of Hepatocellular Carcinoma Cells1000-857810.3971/j.issn.1000-8578.2021.21.0261https://doaj.org/article/f6393d777b2d4b9da477229f535e9fef2021-10-01T00:00:00Zhttp://html.rhhz.net/ZLFZYJ/html/8578.2021.21.0261.htmhttps://doaj.org/toc/1000-8578Objective To use in vitro experiments to verify the changes of proliferation, senescence and apoptosis of hepatocellular carcinoma cells after inhibiting the expression of NEK7, and to explore the related molecular mechanism. Methods Western blot and RT-PCR were used to detect the expression of NEK7 in hepatocellular carcinoma cells and THLE-2 cells. A viral vector was designed to inhibit the expression of NEK7 based on the gene sequence. After hepatocellular carcinoma cells were transfected, we observed the changes of proliferation activity, cell senescence, cell apoptosis and cell cycle in vitro. Western blot was used to detect the expression of cell cycle-related factors. Results Compared with THLE-2 cells, NEK7 was highly expressed in hepatocellular carcinoma cells. After inhibiting the expression of NEK7 with shRNA, the proliferation of hepatocellular carcinoma cells was inhibited, the proportions of cell senescence and apoptosis were increased, meanwhile, the cell number in stage S and G2/M was significantly reduced, the cell cycle progression was blocked, the expression levels of C-myc, c-Fos, cyclin D1 and cyclin E were inhibited, P16 and P27 expression were increased, and CDK2, CDK4 and CDK6 expression were not significantly changed. Conclusion After inhibiting the expression of NEK7, the proliferation ability of hepatocellular carcinoma cells is reduced, cell senescence is promoted and apoptosis is induced; meanwhile, the cell cycle progress is blocked.SONG YanzhouZHANG KunCHEN QijunWEI WenpingZHAO XinLI ZhiweiLI WeiMagazine House of Cancer Research on Prevention and Treatmentarticlehepatocellular carcinomanek7cell cycleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ZHZhongliu Fangzhi Yanjiu, Vol 48, Iss 10, Pp 929-933 (2021)
institution	DOAJ
collection	DOAJ
language	ZH
topic	hepatocellular carcinoma nek7 cell cycle Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	hepatocellular carcinoma nek7 cell cycle Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 SONG Yanzhou ZHANG Kun CHEN Qijun WEI Wenping ZHAO Xin LI Zhiwei LI Wei Experiment on Inhibiting NEK7 to Promote Apoptosis of Hepatocellular Carcinoma Cells
description	Objective To use in vitro experiments to verify the changes of proliferation, senescence and apoptosis of hepatocellular carcinoma cells after inhibiting the expression of NEK7, and to explore the related molecular mechanism. Methods Western blot and RT-PCR were used to detect the expression of NEK7 in hepatocellular carcinoma cells and THLE-2 cells. A viral vector was designed to inhibit the expression of NEK7 based on the gene sequence. After hepatocellular carcinoma cells were transfected, we observed the changes of proliferation activity, cell senescence, cell apoptosis and cell cycle in vitro. Western blot was used to detect the expression of cell cycle-related factors. Results Compared with THLE-2 cells, NEK7 was highly expressed in hepatocellular carcinoma cells. After inhibiting the expression of NEK7 with shRNA, the proliferation of hepatocellular carcinoma cells was inhibited, the proportions of cell senescence and apoptosis were increased, meanwhile, the cell number in stage S and G2/M was significantly reduced, the cell cycle progression was blocked, the expression levels of C-myc, c-Fos, cyclin D1 and cyclin E were inhibited, P16 and P27 expression were increased, and CDK2, CDK4 and CDK6 expression were not significantly changed. Conclusion After inhibiting the expression of NEK7, the proliferation ability of hepatocellular carcinoma cells is reduced, cell senescence is promoted and apoptosis is induced; meanwhile, the cell cycle progress is blocked.
format	article
author	SONG Yanzhou ZHANG Kun CHEN Qijun WEI Wenping ZHAO Xin LI Zhiwei LI Wei
author_facet	SONG Yanzhou ZHANG Kun CHEN Qijun WEI Wenping ZHAO Xin LI Zhiwei LI Wei
author_sort	SONG Yanzhou
title	Experiment on Inhibiting NEK7 to Promote Apoptosis of Hepatocellular Carcinoma Cells
title_short	Experiment on Inhibiting NEK7 to Promote Apoptosis of Hepatocellular Carcinoma Cells
title_full	Experiment on Inhibiting NEK7 to Promote Apoptosis of Hepatocellular Carcinoma Cells
title_fullStr	Experiment on Inhibiting NEK7 to Promote Apoptosis of Hepatocellular Carcinoma Cells
title_full_unstemmed	Experiment on Inhibiting NEK7 to Promote Apoptosis of Hepatocellular Carcinoma Cells
title_sort	experiment on inhibiting nek7 to promote apoptosis of hepatocellular carcinoma cells
publisher	Magazine House of Cancer Research on Prevention and Treatment
publishDate	2021
url	https://doaj.org/article/f6393d777b2d4b9da477229f535e9fef
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Experiment on Inhibiting NEK7 to Promote Apoptosis of Hepatocellular Carcinoma Cells

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