Bacterial processing of glucose modulates C. elegans lifespan and healthspan

Abstract Intestinal microbiota play an essential role in the health of a host organism. Here, we define how commensal Escherichia coli (E. coli) alters its host after long term exposure to glucose using a Caenorhabditis elegans-E. coli system where only the bacteria have direct contact with glucose....

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Autores principales:	Samuel F. Kingsley, Yonghak Seo, Calista Allen, Krishna S. Ghanta, Steven Finkel, Heidi A. Tissenbaum
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f63aae9db4f64f86b521e0dee1a092a0
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spelling	oai:doaj.org-article:f63aae9db4f64f86b521e0dee1a092a02021-12-02T16:30:58ZBacterial processing of glucose modulates C. elegans lifespan and healthspan10.1038/s41598-021-85046-32045-2322https://doaj.org/article/f63aae9db4f64f86b521e0dee1a092a02021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85046-3https://doaj.org/toc/2045-2322Abstract Intestinal microbiota play an essential role in the health of a host organism. Here, we define how commensal Escherichia coli (E. coli) alters its host after long term exposure to glucose using a Caenorhabditis elegans-E. coli system where only the bacteria have direct contact with glucose. Our data reveal that bacterial processing of glucose results in reduced lifespan and healthspan including reduced locomotion, oxidative stress resistance, and heat stress resistance in C. elegans. With chronic exposure to glucose, E. coli exhibits growth defects and increased advanced glycation end products. These negative effects are abrogated when the E. coli is not able to process the additional glucose and by the addition of the anti-glycation compound carnosine. Physiological changes of the host C. elegans are accompanied by dysregulation of detoxifying genes including glyoxalase, glutathione-S-transferase, and superoxide dismutase. Loss of the glutathione-S-transferase, gst-4 shortens C. elegans lifespan and blunts the animal's response to a glucose fed bacterial diet. Taken together, we reveal that added dietary sugar may alter intestinal microbial E. coli to decrease lifespan and healthspan of the host and define a critical role of detoxification genes in maintaining health during a chronic high-sugar diet.Samuel F. KingsleyYonghak SeoCalista AllenKrishna S. GhantaSteven FinkelHeidi A. TissenbaumNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Samuel F. Kingsley Yonghak Seo Calista Allen Krishna S. Ghanta Steven Finkel Heidi A. Tissenbaum Bacterial processing of glucose modulates C. elegans lifespan and healthspan
description	Abstract Intestinal microbiota play an essential role in the health of a host organism. Here, we define how commensal Escherichia coli (E. coli) alters its host after long term exposure to glucose using a Caenorhabditis elegans-E. coli system where only the bacteria have direct contact with glucose. Our data reveal that bacterial processing of glucose results in reduced lifespan and healthspan including reduced locomotion, oxidative stress resistance, and heat stress resistance in C. elegans. With chronic exposure to glucose, E. coli exhibits growth defects and increased advanced glycation end products. These negative effects are abrogated when the E. coli is not able to process the additional glucose and by the addition of the anti-glycation compound carnosine. Physiological changes of the host C. elegans are accompanied by dysregulation of detoxifying genes including glyoxalase, glutathione-S-transferase, and superoxide dismutase. Loss of the glutathione-S-transferase, gst-4 shortens C. elegans lifespan and blunts the animal's response to a glucose fed bacterial diet. Taken together, we reveal that added dietary sugar may alter intestinal microbial E. coli to decrease lifespan and healthspan of the host and define a critical role of detoxification genes in maintaining health during a chronic high-sugar diet.
format	article
author	Samuel F. Kingsley Yonghak Seo Calista Allen Krishna S. Ghanta Steven Finkel Heidi A. Tissenbaum
author_facet	Samuel F. Kingsley Yonghak Seo Calista Allen Krishna S. Ghanta Steven Finkel Heidi A. Tissenbaum
author_sort	Samuel F. Kingsley
title	Bacterial processing of glucose modulates C. elegans lifespan and healthspan
title_short	Bacterial processing of glucose modulates C. elegans lifespan and healthspan
title_full	Bacterial processing of glucose modulates C. elegans lifespan and healthspan
title_fullStr	Bacterial processing of glucose modulates C. elegans lifespan and healthspan
title_full_unstemmed	Bacterial processing of glucose modulates C. elegans lifespan and healthspan
title_sort	bacterial processing of glucose modulates c. elegans lifespan and healthspan
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f63aae9db4f64f86b521e0dee1a092a0
work_keys_str_mv	AT samuelfkingsley bacterialprocessingofglucosemodulatesceleganslifespanandhealthspan AT yonghakseo bacterialprocessingofglucosemodulatesceleganslifespanandhealthspan AT calistaallen bacterialprocessingofglucosemodulatesceleganslifespanandhealthspan AT krishnasghanta bacterialprocessingofglucosemodulatesceleganslifespanandhealthspan AT stevenfinkel bacterialprocessingofglucosemodulatesceleganslifespanandhealthspan AT heidiatissenbaum bacterialprocessingofglucosemodulatesceleganslifespanandhealthspan
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Bacterial processing of glucose modulates C. elegans lifespan and healthspan

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