Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits

Data on the values of selected blood antioxidant parameters, i.e. total antioxidant capacity, glutathione peroxidase, and superoxide dismutase in healthy dogs, are lacking. There are no published accepted standard reference methods for their determination. The aim of this study was to determine the...

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Autores principales:	Tomsič Katerina, Seliškar Alenka, Lukanc Barbara, Nemec Svete Alenka
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2016
Materias:	antioxidant enzymes automated assays canine oxidative stress total antioxidant status Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/f63d36e95482498989ef6d3aa2b92c58
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spelling	oai:doaj.org-article:f63d36e95482498989ef6d3aa2b92c582021-11-17T21:27:51ZPlasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits1820-744810.1515/acve-2016-0046https://doaj.org/article/f63d36e95482498989ef6d3aa2b92c582016-12-01T00:00:00Zhttps://doi.org/10.1515/acve-2016-0046https://doaj.org/toc/1820-7448Data on the values of selected blood antioxidant parameters, i.e. total antioxidant capacity, glutathione peroxidase, and superoxide dismutase in healthy dogs, are lacking. There are no published accepted standard reference methods for their determination. The aim of this study was to determine the values of plasma total antioxidant capacity and the activities of whole blood glutathione peroxidase and erythrocyte superoxide dismutase in 30 healthy client-owned dogs (19 females, 11 males). The effect of age and sex on the measured antioxidant parameters was also investigated. Antioxidant parameters were determined with an automated biochemical analyser, using the commercially available Randox kits. No significant difference in age, weight, and antioxidant parameters was determined between females and males. A significant positive effect of age (p = 0.002, r2 = 0.284) on superoxide dismutase activity was confirmed. There was no effect of sex on any of the antioxidant parameters measured. However, we observed a tendency of the effect of sex (p = 0.063, r2 = 0.118), as well as age (p = 0.073, r2 = 0.111), on the activity of glutathione peroxidase. Our results are in part comparable with the results of other studies in which the same types of methods and samples were used to determine antioxidant parameters. In conclusion, the sex and age of dogs should be taken into consideration when planning a study on antioxidant status parameters.Tomsič KaterinaSeliškar AlenkaLukanc BarbaraNemec Svete AlenkaSciendoarticleantioxidant enzymesautomated assayscanineoxidative stresstotal antioxidant statusVeterinary medicineSF600-1100ENActa Veterinaria, Vol 66, Iss 4, Pp 534-548 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	antioxidant enzymes automated assays canine oxidative stress total antioxidant status Veterinary medicine SF600-1100
spellingShingle	antioxidant enzymes automated assays canine oxidative stress total antioxidant status Veterinary medicine SF600-1100 Tomsič Katerina Seliškar Alenka Lukanc Barbara Nemec Svete Alenka Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits
description	Data on the values of selected blood antioxidant parameters, i.e. total antioxidant capacity, glutathione peroxidase, and superoxide dismutase in healthy dogs, are lacking. There are no published accepted standard reference methods for their determination. The aim of this study was to determine the values of plasma total antioxidant capacity and the activities of whole blood glutathione peroxidase and erythrocyte superoxide dismutase in 30 healthy client-owned dogs (19 females, 11 males). The effect of age and sex on the measured antioxidant parameters was also investigated. Antioxidant parameters were determined with an automated biochemical analyser, using the commercially available Randox kits. No significant difference in age, weight, and antioxidant parameters was determined between females and males. A significant positive effect of age (p = 0.002, r2 = 0.284) on superoxide dismutase activity was confirmed. There was no effect of sex on any of the antioxidant parameters measured. However, we observed a tendency of the effect of sex (p = 0.063, r2 = 0.118), as well as age (p = 0.073, r2 = 0.111), on the activity of glutathione peroxidase. Our results are in part comparable with the results of other studies in which the same types of methods and samples were used to determine antioxidant parameters. In conclusion, the sex and age of dogs should be taken into consideration when planning a study on antioxidant status parameters.
format	article
author	Tomsič Katerina Seliškar Alenka Lukanc Barbara Nemec Svete Alenka
author_facet	Tomsič Katerina Seliškar Alenka Lukanc Barbara Nemec Svete Alenka
author_sort	Tomsič Katerina
title	Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits
title_short	Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits
title_full	Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits
title_fullStr	Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits
title_full_unstemmed	Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits
title_sort	plasma total antioxidant capacity and activities of blood glutathione peroxidase and superoxide dismutase determined in healthy dogs by using commercially available kits
publisher	Sciendo
publishDate	2016
url	https://doaj.org/article/f63d36e95482498989ef6d3aa2b92c58
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Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits

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