Gene expression profiles identify inflammatory signatures in dendritic cells.

Dendritic cells (DCs) constitute a heterogeneous group of antigen-presenting leukocytes important in activation of both innate and adaptive immunity. We studied the gene expression patterns of DCs incubated with reagents inducing their activation or inhibition. Total RNA was isolated from DCs and ge...

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Autores principales:	Anna Torri, Ottavio Beretta, Anna Ranghetti, Francesca Granucci, Paola Ricciardi-Castagnoli, Maria Foti
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f63d8d2b66b14e60baa30c9cb3916597
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spelling	oai:doaj.org-article:f63d8d2b66b14e60baa30c9cb39165972021-11-25T06:25:34ZGene expression profiles identify inflammatory signatures in dendritic cells.1932-620310.1371/journal.pone.0009404https://doaj.org/article/f63d8d2b66b14e60baa30c9cb39165972010-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20195376/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Dendritic cells (DCs) constitute a heterogeneous group of antigen-presenting leukocytes important in activation of both innate and adaptive immunity. We studied the gene expression patterns of DCs incubated with reagents inducing their activation or inhibition. Total RNA was isolated from DCs and gene expression profiling was performed with oligonucleotide microarrays. Using a supervised learning algorithm based on Random Forest, we generated a molecular signature of inflammation from a training set of 77 samples. We then validated this molecular signature in a testing set of 38 samples. Supervised analysis identified a set of 44 genes that distinguished very accurately between inflammatory and non inflammatory samples. The diagnostic performance of the signature genes was assessed against an independent set of samples, by qRT-PCR. Our findings suggest that the gene expression signature of DCs can provide a molecular classification for use in the selection of anti-inflammatory or adjuvant molecules with specific effects on DC activity.Anna TorriOttavio BerettaAnna RanghettiFrancesca GranucciPaola Ricciardi-CastagnoliMaria FotiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 2, p e9404 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna Torri Ottavio Beretta Anna Ranghetti Francesca Granucci Paola Ricciardi-Castagnoli Maria Foti Gene expression profiles identify inflammatory signatures in dendritic cells.
description	Dendritic cells (DCs) constitute a heterogeneous group of antigen-presenting leukocytes important in activation of both innate and adaptive immunity. We studied the gene expression patterns of DCs incubated with reagents inducing their activation or inhibition. Total RNA was isolated from DCs and gene expression profiling was performed with oligonucleotide microarrays. Using a supervised learning algorithm based on Random Forest, we generated a molecular signature of inflammation from a training set of 77 samples. We then validated this molecular signature in a testing set of 38 samples. Supervised analysis identified a set of 44 genes that distinguished very accurately between inflammatory and non inflammatory samples. The diagnostic performance of the signature genes was assessed against an independent set of samples, by qRT-PCR. Our findings suggest that the gene expression signature of DCs can provide a molecular classification for use in the selection of anti-inflammatory or adjuvant molecules with specific effects on DC activity.
format	article
author	Anna Torri Ottavio Beretta Anna Ranghetti Francesca Granucci Paola Ricciardi-Castagnoli Maria Foti
author_facet	Anna Torri Ottavio Beretta Anna Ranghetti Francesca Granucci Paola Ricciardi-Castagnoli Maria Foti
author_sort	Anna Torri
title	Gene expression profiles identify inflammatory signatures in dendritic cells.
title_short	Gene expression profiles identify inflammatory signatures in dendritic cells.
title_full	Gene expression profiles identify inflammatory signatures in dendritic cells.
title_fullStr	Gene expression profiles identify inflammatory signatures in dendritic cells.
title_full_unstemmed	Gene expression profiles identify inflammatory signatures in dendritic cells.
title_sort	gene expression profiles identify inflammatory signatures in dendritic cells.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/f63d8d2b66b14e60baa30c9cb3916597
work_keys_str_mv	AT annatorri geneexpressionprofilesidentifyinflammatorysignaturesindendriticcells AT ottavioberetta geneexpressionprofilesidentifyinflammatorysignaturesindendriticcells AT annaranghetti geneexpressionprofilesidentifyinflammatorysignaturesindendriticcells AT francescagranucci geneexpressionprofilesidentifyinflammatorysignaturesindendriticcells AT paolaricciardicastagnoli geneexpressionprofilesidentifyinflammatorysignaturesindendriticcells AT mariafoti geneexpressionprofilesidentifyinflammatorysignaturesindendriticcells
_version_	1718413779989954560

Gene expression profiles identify inflammatory signatures in dendritic cells.

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