p-Coumaric acid regulates macrophage polarization in myocardial ischemia/reperfusion by promoting the expression of indoleamine 2, 3-dioxygenase

Macrophage infiltration is a hallmark pathological change observed in early stage myocardial ischemia/reperfusion (MI/R) injury and one of the main causes of myocardial damage. Here, we investigated the effects of p-Coumaric acid (p-CA) on macrophage polarization following MI/R injury and its mechan...

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Autores principales:	Na Li, Xueyuan Guo, Rui Li, Jian Zhou, Fangfang Yu, Xianliang Yan
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	p-coumaric acid macrophage polarization myocardial ischemia/reperfusion ido Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/f640e04d4bab4fe7bd2f92a02396ae90
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spelling	oai:doaj.org-article:f640e04d4bab4fe7bd2f92a02396ae902021-12-01T14:41:00Zp-Coumaric acid regulates macrophage polarization in myocardial ischemia/reperfusion by promoting the expression of indoleamine 2, 3-dioxygenase2165-59792165-598710.1080/21655979.2021.2001924https://doaj.org/article/f640e04d4bab4fe7bd2f92a02396ae902021-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2001924https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Macrophage infiltration is a hallmark pathological change observed in early stage myocardial ischemia/reperfusion (MI/R) injury and one of the main causes of myocardial damage. Here, we investigated the effects of p-Coumaric acid (p-CA) on macrophage polarization following MI/R injury and its mechanisms. In vitro, p-CA decreases the expression of LPS/IFN-γ-induced M1 macrophage markers (TNF-α, IL-6, iNOS and CCL2) and increases IL-4-induced M2 macrophage markers (IL-10, CD206, Arg1 and Mrc) in mouse bone marrow-derived macrophages (BMDMs). Additionally, p-CA elevated indoleamine 2, 3-dioxygenase (IDO) protein expression levels, M2 macrophage polarization and M2 macrophage markers through IL-4. In contrast, repression of IDO attenuated p-CA functions regulating BMDMs through IL-4. In vivo, IDO expression was downregulated in mouse hearts subjected to MI/R injury. Treatment of p-CA increased IDO expression in the hearts of MI/R mice. Functionally, p-CA decreases M1 macrophage markers, the number of M1 macrophages and inflammation around heart tissue following MI/R injury. Importantly, p-CA reduces cardiomyocyte apoptosis caused by MI/R. Altogether, our study identified that p-CA modulates macrophage polarization by promoting IDO expression and that p-CA attenuates macrophage-mediated inflammation following MI/R by promoting M2 macrophage polarization through IDO.Na LiXueyuan GuoRui LiJian ZhouFangfang YuXianliang YanTaylor & Francis Grouparticlep-coumaric acidmacrophage polarizationmyocardial ischemia/reperfusionidoBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 2, Pp 10971-10981 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	p-coumaric acid macrophage polarization myocardial ischemia/reperfusion ido Biotechnology TP248.13-248.65
spellingShingle	p-coumaric acid macrophage polarization myocardial ischemia/reperfusion ido Biotechnology TP248.13-248.65 Na Li Xueyuan Guo Rui Li Jian Zhou Fangfang Yu Xianliang Yan p-Coumaric acid regulates macrophage polarization in myocardial ischemia/reperfusion by promoting the expression of indoleamine 2, 3-dioxygenase
description	Macrophage infiltration is a hallmark pathological change observed in early stage myocardial ischemia/reperfusion (MI/R) injury and one of the main causes of myocardial damage. Here, we investigated the effects of p-Coumaric acid (p-CA) on macrophage polarization following MI/R injury and its mechanisms. In vitro, p-CA decreases the expression of LPS/IFN-γ-induced M1 macrophage markers (TNF-α, IL-6, iNOS and CCL2) and increases IL-4-induced M2 macrophage markers (IL-10, CD206, Arg1 and Mrc) in mouse bone marrow-derived macrophages (BMDMs). Additionally, p-CA elevated indoleamine 2, 3-dioxygenase (IDO) protein expression levels, M2 macrophage polarization and M2 macrophage markers through IL-4. In contrast, repression of IDO attenuated p-CA functions regulating BMDMs through IL-4. In vivo, IDO expression was downregulated in mouse hearts subjected to MI/R injury. Treatment of p-CA increased IDO expression in the hearts of MI/R mice. Functionally, p-CA decreases M1 macrophage markers, the number of M1 macrophages and inflammation around heart tissue following MI/R injury. Importantly, p-CA reduces cardiomyocyte apoptosis caused by MI/R. Altogether, our study identified that p-CA modulates macrophage polarization by promoting IDO expression and that p-CA attenuates macrophage-mediated inflammation following MI/R by promoting M2 macrophage polarization through IDO.
format	article
author	Na Li Xueyuan Guo Rui Li Jian Zhou Fangfang Yu Xianliang Yan
author_facet	Na Li Xueyuan Guo Rui Li Jian Zhou Fangfang Yu Xianliang Yan
author_sort	Na Li
title	p-Coumaric acid regulates macrophage polarization in myocardial ischemia/reperfusion by promoting the expression of indoleamine 2, 3-dioxygenase
title_short	p-Coumaric acid regulates macrophage polarization in myocardial ischemia/reperfusion by promoting the expression of indoleamine 2, 3-dioxygenase
title_full	p-Coumaric acid regulates macrophage polarization in myocardial ischemia/reperfusion by promoting the expression of indoleamine 2, 3-dioxygenase
title_fullStr	p-Coumaric acid regulates macrophage polarization in myocardial ischemia/reperfusion by promoting the expression of indoleamine 2, 3-dioxygenase
title_full_unstemmed	p-Coumaric acid regulates macrophage polarization in myocardial ischemia/reperfusion by promoting the expression of indoleamine 2, 3-dioxygenase
title_sort	p-coumaric acid regulates macrophage polarization in myocardial ischemia/reperfusion by promoting the expression of indoleamine 2, 3-dioxygenase
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/f640e04d4bab4fe7bd2f92a02396ae90
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p-Coumaric acid regulates macrophage polarization in myocardial ischemia/reperfusion by promoting the expression of indoleamine 2, 3-dioxygenase

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