A novel circular RNA circ_HN1/miR-628-5p/Ecto-5’-nucleotidase competing endogenous RNA network regulates gastric cancer development

The competing endogenous RNA (ceRNA) activity of circular RNAs (circRNAs) has been implicated in the development of gastric cancer. Here, we sought to explore the ceRNA function of circRNA Jupiter microtubule associated homolog 1 (circ_HN1) in gastric tumorigenesis. Circ_HN1, microRNA (miR)-628-5p,...

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Autores principales:	Jianmin Zhang, Fang Wang, Haihui Zhang, Mingbo Cao
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	circ_hn1 mir-628-5p nt5e cerna crosstalk gastric cancer Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/f642f029763b4326a36280bf21b39fd7
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spelling	oai:doaj.org-article:f642f029763b4326a36280bf21b39fd72021-12-01T14:41:00ZA novel circular RNA circ_HN1/miR-628-5p/Ecto-5’-nucleotidase competing endogenous RNA network regulates gastric cancer development2165-59792165-598710.1080/21655979.2021.1989259https://doaj.org/article/f642f029763b4326a36280bf21b39fd72021-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1989259https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987The competing endogenous RNA (ceRNA) activity of circular RNAs (circRNAs) has been implicated in the development of gastric cancer. Here, we sought to explore the ceRNA function of circRNA Jupiter microtubule associated homolog 1 (circ_HN1) in gastric tumorigenesis. Circ_HN1, microRNA (miR)-628-5p, and NT5E expression levels were quantified by qRT-PCR and western blot. Dual-luciferase reporter assays were used to assess the direct relationship between miR-628-5p and circ_HN1 or NT5E. Our data showed that circ_HN1 expression was enhanced in human gastric cancer. Depletion of circ_HN1 impeded cell proliferation, spheroid formation, invasion, and migration and promoted apoptosis in vitro, as well as diminished tumor growth in vivo. NT5E was a downstream effector of circ_HN1 function. NT5E was targeted and inhibited by miR-628-5p through the perfect complementary site in NT5E 3ʹUTR, and circ_HN1 affected NT5E expression through miR-628-5p competition. Moreover, depletion of miR-628-5p reversed the effects of circ_HN1 silencing on regulating cell functional behaviors. Our findings identify a novel ceRNA network, the circ_HN1/miR-628-5p/NT5E axis, for the oncogenic activity of circ_HN1 in gastric cancer, highlighting circ_HN1 inhibition as a promising targeted treatment against gastric cancer.Jianmin ZhangFang WangHaihui ZhangMingbo CaoTaylor & Francis Grouparticlecirc_hn1mir-628-5pnt5ecerna crosstalkgastric cancerBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 2, Pp 9739-9752 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circ_hn1 mir-628-5p nt5e cerna crosstalk gastric cancer Biotechnology TP248.13-248.65
spellingShingle	circ_hn1 mir-628-5p nt5e cerna crosstalk gastric cancer Biotechnology TP248.13-248.65 Jianmin Zhang Fang Wang Haihui Zhang Mingbo Cao A novel circular RNA circ_HN1/miR-628-5p/Ecto-5’-nucleotidase competing endogenous RNA network regulates gastric cancer development
description	The competing endogenous RNA (ceRNA) activity of circular RNAs (circRNAs) has been implicated in the development of gastric cancer. Here, we sought to explore the ceRNA function of circRNA Jupiter microtubule associated homolog 1 (circ_HN1) in gastric tumorigenesis. Circ_HN1, microRNA (miR)-628-5p, and NT5E expression levels were quantified by qRT-PCR and western blot. Dual-luciferase reporter assays were used to assess the direct relationship between miR-628-5p and circ_HN1 or NT5E. Our data showed that circ_HN1 expression was enhanced in human gastric cancer. Depletion of circ_HN1 impeded cell proliferation, spheroid formation, invasion, and migration and promoted apoptosis in vitro, as well as diminished tumor growth in vivo. NT5E was a downstream effector of circ_HN1 function. NT5E was targeted and inhibited by miR-628-5p through the perfect complementary site in NT5E 3ʹUTR, and circ_HN1 affected NT5E expression through miR-628-5p competition. Moreover, depletion of miR-628-5p reversed the effects of circ_HN1 silencing on regulating cell functional behaviors. Our findings identify a novel ceRNA network, the circ_HN1/miR-628-5p/NT5E axis, for the oncogenic activity of circ_HN1 in gastric cancer, highlighting circ_HN1 inhibition as a promising targeted treatment against gastric cancer.
format	article
author	Jianmin Zhang Fang Wang Haihui Zhang Mingbo Cao
author_facet	Jianmin Zhang Fang Wang Haihui Zhang Mingbo Cao
author_sort	Jianmin Zhang
title	A novel circular RNA circ_HN1/miR-628-5p/Ecto-5’-nucleotidase competing endogenous RNA network regulates gastric cancer development
title_short	A novel circular RNA circ_HN1/miR-628-5p/Ecto-5’-nucleotidase competing endogenous RNA network regulates gastric cancer development
title_full	A novel circular RNA circ_HN1/miR-628-5p/Ecto-5’-nucleotidase competing endogenous RNA network regulates gastric cancer development
title_fullStr	A novel circular RNA circ_HN1/miR-628-5p/Ecto-5’-nucleotidase competing endogenous RNA network regulates gastric cancer development
title_full_unstemmed	A novel circular RNA circ_HN1/miR-628-5p/Ecto-5’-nucleotidase competing endogenous RNA network regulates gastric cancer development
title_sort	novel circular rna circ_hn1/mir-628-5p/ecto-5’-nucleotidase competing endogenous rna network regulates gastric cancer development
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/f642f029763b4326a36280bf21b39fd7
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A novel circular RNA circ_HN1/miR-628-5p/Ecto-5’-nucleotidase competing endogenous RNA network regulates gastric cancer development

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