vB_BcM_Sam46 and vB_BcM_Sam112, members of a new bacteriophage genus with unusual small terminase structure

Abstract One of the serious public health concerns is food contaminated with pathogens and their vital activity products such as toxins. Bacillus cereus group of bacteria includes well-known pathogenic species such as B. anthracis, B. cereus sensu stricto (ss), B. cytotoxicus and B. thuringiensis. I...

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Autores principales:	Olesya A. Kazantseva, Emma G. Piligrimova, Andrey M. Shadrin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f649957710c041338475d4b53f4311c5
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spelling	oai:doaj.org-article:f649957710c041338475d4b53f4311c52021-12-02T17:48:00ZvB_BcM_Sam46 and vB_BcM_Sam112, members of a new bacteriophage genus with unusual small terminase structure10.1038/s41598-021-91289-x2045-2322https://doaj.org/article/f649957710c041338475d4b53f4311c52021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91289-xhttps://doaj.org/toc/2045-2322Abstract One of the serious public health concerns is food contaminated with pathogens and their vital activity products such as toxins. Bacillus cereus group of bacteria includes well-known pathogenic species such as B. anthracis, B. cereus sensu stricto (ss), B. cytotoxicus and B. thuringiensis. In this report, we describe the Bacillus phages vB_BcM_Sam46 and vB_BcM_Sam112 infecting species of this group. Electron microscopic analyses indicated that phages Sam46 and Sam112 have the myovirus morphotype. The genomes of Sam46 and Sam112 comprise double-stranded DNA of 45,419 bp and 45,037 bp in length, respectively, and have the same GC-content. The genome identity of Sam46 and Sam112 is 96.0%, indicating that they belong to the same phage species. According to the phylogenetic analysis, these phages form a distinct clade and may be members of a new phage genus, for which we propose the name ‘Samaravirus’. In addition, an interesting feature of the Sam46 and Sam112 phages is the unusual structure of their small terminase subunit containing N-terminal FtsK_gamma domain.Olesya A. KazantsevaEmma G. PiligrimovaAndrey M. ShadrinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Olesya A. Kazantseva Emma G. Piligrimova Andrey M. Shadrin vB_BcM_Sam46 and vB_BcM_Sam112, members of a new bacteriophage genus with unusual small terminase structure
description	Abstract One of the serious public health concerns is food contaminated with pathogens and their vital activity products such as toxins. Bacillus cereus group of bacteria includes well-known pathogenic species such as B. anthracis, B. cereus sensu stricto (ss), B. cytotoxicus and B. thuringiensis. In this report, we describe the Bacillus phages vB_BcM_Sam46 and vB_BcM_Sam112 infecting species of this group. Electron microscopic analyses indicated that phages Sam46 and Sam112 have the myovirus morphotype. The genomes of Sam46 and Sam112 comprise double-stranded DNA of 45,419 bp and 45,037 bp in length, respectively, and have the same GC-content. The genome identity of Sam46 and Sam112 is 96.0%, indicating that they belong to the same phage species. According to the phylogenetic analysis, these phages form a distinct clade and may be members of a new phage genus, for which we propose the name ‘Samaravirus’. In addition, an interesting feature of the Sam46 and Sam112 phages is the unusual structure of their small terminase subunit containing N-terminal FtsK_gamma domain.
format	article
author	Olesya A. Kazantseva Emma G. Piligrimova Andrey M. Shadrin
author_facet	Olesya A. Kazantseva Emma G. Piligrimova Andrey M. Shadrin
author_sort	Olesya A. Kazantseva
title	vB_BcM_Sam46 and vB_BcM_Sam112, members of a new bacteriophage genus with unusual small terminase structure
title_short	vB_BcM_Sam46 and vB_BcM_Sam112, members of a new bacteriophage genus with unusual small terminase structure
title_full	vB_BcM_Sam46 and vB_BcM_Sam112, members of a new bacteriophage genus with unusual small terminase structure
title_fullStr	vB_BcM_Sam46 and vB_BcM_Sam112, members of a new bacteriophage genus with unusual small terminase structure
title_full_unstemmed	vB_BcM_Sam46 and vB_BcM_Sam112, members of a new bacteriophage genus with unusual small terminase structure
title_sort	vb_bcm_sam46 and vb_bcm_sam112, members of a new bacteriophage genus with unusual small terminase structure
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f649957710c041338475d4b53f4311c5
work_keys_str_mv	AT olesyaakazantseva vbbcmsam46andvbbcmsam112membersofanewbacteriophagegenuswithunusualsmallterminasestructure AT emmagpiligrimova vbbcmsam46andvbbcmsam112membersofanewbacteriophagegenuswithunusualsmallterminasestructure AT andreymshadrin vbbcmsam46andvbbcmsam112membersofanewbacteriophagegenuswithunusualsmallterminasestructure
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vB_BcM_Sam46 and vB_BcM_Sam112, members of a new bacteriophage genus with unusual small terminase structure

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