RAPID URINE SCREENING FOR BACTERIURIA IN CHILDREN USING MICROBIOLOGY ANALYZER, COMBINING PHOTOMETRIC AND COHERENT FLUCTUATION NEPHELOMETRIC METHODS

One of the most widespread microbiological analyses in clinical practice is urine culture, and up to 80% of urine samples turn out to be negative. In this study we demonstrate applicability of coherent fluctuating nephelometry (CFN) method for rapid urine screening and negative samples identificatio...

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Autores principales: A. S. Gur’ev, O. Yu. Kuznetsova, M. F. Pyasetskaya, I. A. Smirnova, N. A. Belyaeva, V. N. Verbov, A. Yu. Volkov
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Lenguaje:RU
Publicado: Sankt-Peterburg : NIIÈM imeni Pastera 2017
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Acceso en línea:https://doaj.org/article/f64d15abe20f447684d04e7079afc24b
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spelling oai:doaj.org-article:f64d15abe20f447684d04e7079afc24b2021-11-22T07:09:48ZRAPID URINE SCREENING FOR BACTERIURIA IN CHILDREN USING MICROBIOLOGY ANALYZER, COMBINING PHOTOMETRIC AND COHERENT FLUCTUATION NEPHELOMETRIC METHODS2220-76192313-739810.15789/2220-7619-2016-4-395-398https://doaj.org/article/f64d15abe20f447684d04e7079afc24b2017-01-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/454https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398One of the most widespread microbiological analyses in clinical practice is urine culture, and up to 80% of urine samples turn out to be negative. In this study we demonstrate applicability of coherent fluctuating nephelometry (CFN) method for rapid urine screening and negative samples identification for the purpose of reducing the workload of microbiological laboratory. 205 urine samples were tested by conventional culture method (CCM) and using CFN-analyzer. The agreement between CCM and CFN was 87.8%. Compared to CCM, CFN demonstrated sensitivity — 94.5%, specificity — 85.3%, negative predictive value — 97.7%, positive predictive value — 70.3%. In this study CFN-analyzer allowed to identify 85.3% of negative urine samples (63.9% of all tested samples) within 4 hours, and 94.5% of positive samples were retained for later CCM. Moreover none of samples with count ≥ 104 CFU/ml was omitted.A. S. Gur’evO. Yu. KuznetsovaM. F. PyasetskayaI. A. SmirnovaN. A. BelyaevaV. N. VerbovA. Yu. VolkovSankt-Peterburg : NIIÈM imeni Pasteraarticlecoherent fluctuation nephelometrybacteriuriascreeningurinechildrenmicrobiology analyzerInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 6, Iss 4, Pp 395-398 (2017)
institution DOAJ
collection DOAJ
language RU
topic coherent fluctuation nephelometry
bacteriuria
screening
urine
children
microbiology analyzer
Infectious and parasitic diseases
RC109-216
spellingShingle coherent fluctuation nephelometry
bacteriuria
screening
urine
children
microbiology analyzer
Infectious and parasitic diseases
RC109-216
A. S. Gur’ev
O. Yu. Kuznetsova
M. F. Pyasetskaya
I. A. Smirnova
N. A. Belyaeva
V. N. Verbov
A. Yu. Volkov
RAPID URINE SCREENING FOR BACTERIURIA IN CHILDREN USING MICROBIOLOGY ANALYZER, COMBINING PHOTOMETRIC AND COHERENT FLUCTUATION NEPHELOMETRIC METHODS
description One of the most widespread microbiological analyses in clinical practice is urine culture, and up to 80% of urine samples turn out to be negative. In this study we demonstrate applicability of coherent fluctuating nephelometry (CFN) method for rapid urine screening and negative samples identification for the purpose of reducing the workload of microbiological laboratory. 205 urine samples were tested by conventional culture method (CCM) and using CFN-analyzer. The agreement between CCM and CFN was 87.8%. Compared to CCM, CFN demonstrated sensitivity — 94.5%, specificity — 85.3%, negative predictive value — 97.7%, positive predictive value — 70.3%. In this study CFN-analyzer allowed to identify 85.3% of negative urine samples (63.9% of all tested samples) within 4 hours, and 94.5% of positive samples were retained for later CCM. Moreover none of samples with count ≥ 104 CFU/ml was omitted.
format article
author A. S. Gur’ev
O. Yu. Kuznetsova
M. F. Pyasetskaya
I. A. Smirnova
N. A. Belyaeva
V. N. Verbov
A. Yu. Volkov
author_facet A. S. Gur’ev
O. Yu. Kuznetsova
M. F. Pyasetskaya
I. A. Smirnova
N. A. Belyaeva
V. N. Verbov
A. Yu. Volkov
author_sort A. S. Gur’ev
title RAPID URINE SCREENING FOR BACTERIURIA IN CHILDREN USING MICROBIOLOGY ANALYZER, COMBINING PHOTOMETRIC AND COHERENT FLUCTUATION NEPHELOMETRIC METHODS
title_short RAPID URINE SCREENING FOR BACTERIURIA IN CHILDREN USING MICROBIOLOGY ANALYZER, COMBINING PHOTOMETRIC AND COHERENT FLUCTUATION NEPHELOMETRIC METHODS
title_full RAPID URINE SCREENING FOR BACTERIURIA IN CHILDREN USING MICROBIOLOGY ANALYZER, COMBINING PHOTOMETRIC AND COHERENT FLUCTUATION NEPHELOMETRIC METHODS
title_fullStr RAPID URINE SCREENING FOR BACTERIURIA IN CHILDREN USING MICROBIOLOGY ANALYZER, COMBINING PHOTOMETRIC AND COHERENT FLUCTUATION NEPHELOMETRIC METHODS
title_full_unstemmed RAPID URINE SCREENING FOR BACTERIURIA IN CHILDREN USING MICROBIOLOGY ANALYZER, COMBINING PHOTOMETRIC AND COHERENT FLUCTUATION NEPHELOMETRIC METHODS
title_sort rapid urine screening for bacteriuria in children using microbiology analyzer, combining photometric and coherent fluctuation nephelometric methods
publisher Sankt-Peterburg : NIIÈM imeni Pastera
publishDate 2017
url https://doaj.org/article/f64d15abe20f447684d04e7079afc24b
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