Facile fabrication of antibacterial and antiviral perhydrolase-polydopamine composite coatings

Abstract In situ generation of antibacterial and antiviral agents by harnessing the catalytic activity of enzymes on surfaces provides an effective eco-friendly approach for disinfection. The perhydrolase (AcT) from Mycobacterium smegmatis catalyzes the perhydrolysis of acetate esters to generate th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Li-Sheng Wang, Shirley Xu, Sneha Gopal, Eunsol Kim, Domyoung Kim, Matthew Brier, Kusum Solanki, Jonathan S. Dordick
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f64f9a76d16c46ce93991347d097aebd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f64f9a76d16c46ce93991347d097aebd
record_format	dspace
spelling	oai:doaj.org-article:f64f9a76d16c46ce93991347d097aebd2021-12-02T16:04:36ZFacile fabrication of antibacterial and antiviral perhydrolase-polydopamine composite coatings10.1038/s41598-021-91925-62045-2322https://doaj.org/article/f64f9a76d16c46ce93991347d097aebd2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91925-6https://doaj.org/toc/2045-2322Abstract In situ generation of antibacterial and antiviral agents by harnessing the catalytic activity of enzymes on surfaces provides an effective eco-friendly approach for disinfection. The perhydrolase (AcT) from Mycobacterium smegmatis catalyzes the perhydrolysis of acetate esters to generate the potent disinfectant, peracetic acid (PAA). In the presence of AcT and its two substrates, propylene glycol diacetate and H2O2, sufficient and continuous PAA is generated over an extended time to kill a wide range of bacteria with the enzyme dissolved in aqueous buffer. For extended self-disinfection, however, active and stable AcT bound onto or incorporated into a surface coating is necessary. In the current study, an active, stable and reusable AcT-based coating was developed by incorporating AcT into a polydopamine (PDA) matrix in a single step, thereby forming a biocatalytic composite onto a variety of surfaces. The resulting AcT-PDA composite coatings on glass, metal and epoxy surfaces yielded up to 7-log reduction of Gram-positive and Gram-negative bacteria when in contact with the biocatalytic coating. This composite coating also possessed potent antiviral activity, and dramatically reduced the infectivity of a SARS-CoV-2 pseudovirus within minutes. The single-step approach enables rapid and facile fabrication of enzyme-based disinfectant composite coatings with high activity and stability, which enables reuse following surface washing. As a result, this enzyme-polymer composite technique may serve as a general strategy for preparing antibacterial and antiviral surfaces for applications in health care and common infrastructure safety, such as in schools, the workplace, transportation, etc.Li-Sheng WangShirley XuSneha GopalEunsol KimDomyoung KimMatthew BrierKusum SolankiJonathan S. DordickNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Li-Sheng Wang Shirley Xu Sneha Gopal Eunsol Kim Domyoung Kim Matthew Brier Kusum Solanki Jonathan S. Dordick Facile fabrication of antibacterial and antiviral perhydrolase-polydopamine composite coatings
description	Abstract In situ generation of antibacterial and antiviral agents by harnessing the catalytic activity of enzymes on surfaces provides an effective eco-friendly approach for disinfection. The perhydrolase (AcT) from Mycobacterium smegmatis catalyzes the perhydrolysis of acetate esters to generate the potent disinfectant, peracetic acid (PAA). In the presence of AcT and its two substrates, propylene glycol diacetate and H2O2, sufficient and continuous PAA is generated over an extended time to kill a wide range of bacteria with the enzyme dissolved in aqueous buffer. For extended self-disinfection, however, active and stable AcT bound onto or incorporated into a surface coating is necessary. In the current study, an active, stable and reusable AcT-based coating was developed by incorporating AcT into a polydopamine (PDA) matrix in a single step, thereby forming a biocatalytic composite onto a variety of surfaces. The resulting AcT-PDA composite coatings on glass, metal and epoxy surfaces yielded up to 7-log reduction of Gram-positive and Gram-negative bacteria when in contact with the biocatalytic coating. This composite coating also possessed potent antiviral activity, and dramatically reduced the infectivity of a SARS-CoV-2 pseudovirus within minutes. The single-step approach enables rapid and facile fabrication of enzyme-based disinfectant composite coatings with high activity and stability, which enables reuse following surface washing. As a result, this enzyme-polymer composite technique may serve as a general strategy for preparing antibacterial and antiviral surfaces for applications in health care and common infrastructure safety, such as in schools, the workplace, transportation, etc.
format	article
author	Li-Sheng Wang Shirley Xu Sneha Gopal Eunsol Kim Domyoung Kim Matthew Brier Kusum Solanki Jonathan S. Dordick
author_facet	Li-Sheng Wang Shirley Xu Sneha Gopal Eunsol Kim Domyoung Kim Matthew Brier Kusum Solanki Jonathan S. Dordick
author_sort	Li-Sheng Wang
title	Facile fabrication of antibacterial and antiviral perhydrolase-polydopamine composite coatings
title_short	Facile fabrication of antibacterial and antiviral perhydrolase-polydopamine composite coatings
title_full	Facile fabrication of antibacterial and antiviral perhydrolase-polydopamine composite coatings
title_fullStr	Facile fabrication of antibacterial and antiviral perhydrolase-polydopamine composite coatings
title_full_unstemmed	Facile fabrication of antibacterial and antiviral perhydrolase-polydopamine composite coatings
title_sort	facile fabrication of antibacterial and antiviral perhydrolase-polydopamine composite coatings
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f64f9a76d16c46ce93991347d097aebd
work_keys_str_mv	AT lishengwang facilefabricationofantibacterialandantiviralperhydrolasepolydopaminecompositecoatings AT shirleyxu facilefabricationofantibacterialandantiviralperhydrolasepolydopaminecompositecoatings AT snehagopal facilefabricationofantibacterialandantiviralperhydrolasepolydopaminecompositecoatings AT eunsolkim facilefabricationofantibacterialandantiviralperhydrolasepolydopaminecompositecoatings AT domyoungkim facilefabricationofantibacterialandantiviralperhydrolasepolydopaminecompositecoatings AT matthewbrier facilefabricationofantibacterialandantiviralperhydrolasepolydopaminecompositecoatings AT kusumsolanki facilefabricationofantibacterialandantiviralperhydrolasepolydopaminecompositecoatings AT jonathansdordick facilefabricationofantibacterialandantiviralperhydrolasepolydopaminecompositecoatings
_version_	1718385244674981888

Facile fabrication of antibacterial and antiviral perhydrolase-polydopamine composite coatings

Ejemplares similares