Development of a novel recombinant ELISA for the detection of Crimean-Congo hemorrhagic fever virus IgG antibodies

Abstract Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral infection caused by Crimean-Congo hemorrhagic fever virus (CCHFV). Serological screening of CCHF is important and current ELISA use antigens prepared from virus which is expensive due to requirement of high bio-containment facilit...

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Autores principales:	Sultan Gülce-İz, Nazif Elaldı, Hüseyin Can, Esra Atalay Şahar, Muhammet Karakavuk, Aytül Gül, Gizem Örs Kumoğlu, Aysu Değirmenci Döşkaya, Adnan Yüksel Gürüz, Aykut Özdarendeli, Philip Louis Felgner, Huw Davies, Mert Döşkaya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f65cb262f47142d4bde3de5709ab19ed
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spelling	oai:doaj.org-article:f65cb262f47142d4bde3de5709ab19ed2021-12-02T16:31:02ZDevelopment of a novel recombinant ELISA for the detection of Crimean-Congo hemorrhagic fever virus IgG antibodies10.1038/s41598-021-85323-12045-2322https://doaj.org/article/f65cb262f47142d4bde3de5709ab19ed2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85323-1https://doaj.org/toc/2045-2322Abstract Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral infection caused by Crimean-Congo hemorrhagic fever virus (CCHFV). Serological screening of CCHF is important and current ELISA use antigens prepared from virus which is expensive due to requirement of high bio-containment facilities. In this study, we aimed to develop a new recombinant ELISA. For this purpose, CCHFV genome were expressed as 13 proteins in E. coli and among them abundantly purified recombinant Nucleocapsid protein (rNP) and Mucin-like variable domain (rMLD) were used as antigen in ELISA (Rec-ELISA). Rec-ELISA using rNP, rMLD and a combination of both (rNP/rMLD) were probed with acute (n = 64; collected between days 1 and 7 after onset of symptoms), convalescent (n = 35; collected 8 days after onset of symptoms), consecutive sera (n = 25) of confirmed CCHF cases and control sera (n = 43). The sensitivity and specificity of Rec-ELISA using rNP/rMLD were 73% and 98% in acute cases and 97% and 98% in convalescent cases. The median interquartile absorbance value to discriminate the acute and convalescent phases of CCHF was significantly higher with ELISA using rNP/rMLD (P < 0.0001) compared to rNP (P > 0.05) and rMLD (P = 0.001). These results indicate that the Rec-ELISA using rNP/rMLD may be very useful to diagnose convalescent CCHF cases especially in field studies.Sultan Gülce-İzNazif ElaldıHüseyin CanEsra Atalay ŞaharMuhammet KarakavukAytül GülGizem Örs KumoğluAysu Değirmenci DöşkayaAdnan Yüksel GürüzAykut ÖzdarendeliPhilip Louis FelgnerHuw DaviesMert DöşkayaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sultan Gülce-İz Nazif Elaldı Hüseyin Can Esra Atalay Şahar Muhammet Karakavuk Aytül Gül Gizem Örs Kumoğlu Aysu Değirmenci Döşkaya Adnan Yüksel Gürüz Aykut Özdarendeli Philip Louis Felgner Huw Davies Mert Döşkaya Development of a novel recombinant ELISA for the detection of Crimean-Congo hemorrhagic fever virus IgG antibodies
description	Abstract Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral infection caused by Crimean-Congo hemorrhagic fever virus (CCHFV). Serological screening of CCHF is important and current ELISA use antigens prepared from virus which is expensive due to requirement of high bio-containment facilities. In this study, we aimed to develop a new recombinant ELISA. For this purpose, CCHFV genome were expressed as 13 proteins in E. coli and among them abundantly purified recombinant Nucleocapsid protein (rNP) and Mucin-like variable domain (rMLD) were used as antigen in ELISA (Rec-ELISA). Rec-ELISA using rNP, rMLD and a combination of both (rNP/rMLD) were probed with acute (n = 64; collected between days 1 and 7 after onset of symptoms), convalescent (n = 35; collected 8 days after onset of symptoms), consecutive sera (n = 25) of confirmed CCHF cases and control sera (n = 43). The sensitivity and specificity of Rec-ELISA using rNP/rMLD were 73% and 98% in acute cases and 97% and 98% in convalescent cases. The median interquartile absorbance value to discriminate the acute and convalescent phases of CCHF was significantly higher with ELISA using rNP/rMLD (P < 0.0001) compared to rNP (P > 0.05) and rMLD (P = 0.001). These results indicate that the Rec-ELISA using rNP/rMLD may be very useful to diagnose convalescent CCHF cases especially in field studies.
format	article
author	Sultan Gülce-İz Nazif Elaldı Hüseyin Can Esra Atalay Şahar Muhammet Karakavuk Aytül Gül Gizem Örs Kumoğlu Aysu Değirmenci Döşkaya Adnan Yüksel Gürüz Aykut Özdarendeli Philip Louis Felgner Huw Davies Mert Döşkaya
author_facet	Sultan Gülce-İz Nazif Elaldı Hüseyin Can Esra Atalay Şahar Muhammet Karakavuk Aytül Gül Gizem Örs Kumoğlu Aysu Değirmenci Döşkaya Adnan Yüksel Gürüz Aykut Özdarendeli Philip Louis Felgner Huw Davies Mert Döşkaya
author_sort	Sultan Gülce-İz
title	Development of a novel recombinant ELISA for the detection of Crimean-Congo hemorrhagic fever virus IgG antibodies
title_short	Development of a novel recombinant ELISA for the detection of Crimean-Congo hemorrhagic fever virus IgG antibodies
title_full	Development of a novel recombinant ELISA for the detection of Crimean-Congo hemorrhagic fever virus IgG antibodies
title_fullStr	Development of a novel recombinant ELISA for the detection of Crimean-Congo hemorrhagic fever virus IgG antibodies
title_full_unstemmed	Development of a novel recombinant ELISA for the detection of Crimean-Congo hemorrhagic fever virus IgG antibodies
title_sort	development of a novel recombinant elisa for the detection of crimean-congo hemorrhagic fever virus igg antibodies
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f65cb262f47142d4bde3de5709ab19ed
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Development of a novel recombinant ELISA for the detection of Crimean-Congo hemorrhagic fever virus IgG antibodies

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