L-carnitine ameliorates bile duct ligation induced liver fibrosis via reducing the nitrosative stress in experimental animals: preclinical evidences

Bile duct ligation (BDL) has been extensively used in studying the mechanisms of fibrogenesis and anti-fibrotic drugs. Considering the liver regenerative capacity and the diverse results from BDL, the present study aimed to evaluate the protective effect of L-carnitine on bile duct ligation-induced...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vikram Nimbalkar, Neeraj Vyawahare
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Bile duct ligation Fibrosis ALT AST Hydroxyproline Cytokine Science (General) Q1-390 Social sciences (General) H1-99
Acceso en línea:	https://doaj.org/article/f65d7698b7fd4fc1adb84b9940aeb435
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f65d7698b7fd4fc1adb84b9940aeb435
record_format	dspace
spelling	oai:doaj.org-article:f65d7698b7fd4fc1adb84b9940aeb4352021-12-02T05:03:25ZL-carnitine ameliorates bile duct ligation induced liver fibrosis via reducing the nitrosative stress in experimental animals: preclinical evidences2405-844010.1016/j.heliyon.2021.e08488https://doaj.org/article/f65d7698b7fd4fc1adb84b9940aeb4352021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405844021025913https://doaj.org/toc/2405-8440Bile duct ligation (BDL) has been extensively used in studying the mechanisms of fibrogenesis and anti-fibrotic drugs. Considering the liver regenerative capacity and the diverse results from BDL, the present study aimed to evaluate the protective effect of L-carnitine on bile duct ligation-induced liver fibrosis in experimental rats. Rats were randomly divided into seven groups (n = 6). The bile duct was ligated and serum aspartate transaminase (AST), alanine transaminase (ALT), total bilirubin and albumin, hepatic hydroxyproline (HP), reduced glutathione (GSH), and malondialdehyde (MDA) and cytokines were measured. iNOS expression was measured by using Western blot and finally, liver tissue was processed for histopathological analysis (H&E staining)”. The level of iNOS was increased in the control group, whereas a decrease in the level of iNOS was found in the L-carnitine treated group. In the present study, we found that bile duct ligation in rats showed an increase in body and liver weight, while treatment with carnitine showed normal body and liver weight. Serum AST, ALT, total bilirubin, HP, GSH, MDA, and cytokines were increased in bile duct ligated rats. In addition, L-carnitine treated rats showed a reduction in oxidative stress as well as inhibiting the release of cytokines in a dose-dependent manner and showed protection against bile duct ligation. The study concludes that L-carnitine has a protective effect against the liver fibrosis induced by bile duct ligation.Vikram NimbalkarNeeraj VyawahareElsevierarticleBile duct ligationFibrosisALTASTHydroxyprolineCytokineScience (General)Q1-390Social sciences (General)H1-99ENHeliyon, Vol 7, Iss 11, Pp e08488- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bile duct ligation Fibrosis ALT AST Hydroxyproline Cytokine Science (General) Q1-390 Social sciences (General) H1-99
spellingShingle	Bile duct ligation Fibrosis ALT AST Hydroxyproline Cytokine Science (General) Q1-390 Social sciences (General) H1-99 Vikram Nimbalkar Neeraj Vyawahare L-carnitine ameliorates bile duct ligation induced liver fibrosis via reducing the nitrosative stress in experimental animals: preclinical evidences
description	Bile duct ligation (BDL) has been extensively used in studying the mechanisms of fibrogenesis and anti-fibrotic drugs. Considering the liver regenerative capacity and the diverse results from BDL, the present study aimed to evaluate the protective effect of L-carnitine on bile duct ligation-induced liver fibrosis in experimental rats. Rats were randomly divided into seven groups (n = 6). The bile duct was ligated and serum aspartate transaminase (AST), alanine transaminase (ALT), total bilirubin and albumin, hepatic hydroxyproline (HP), reduced glutathione (GSH), and malondialdehyde (MDA) and cytokines were measured. iNOS expression was measured by using Western blot and finally, liver tissue was processed for histopathological analysis (H&E staining)”. The level of iNOS was increased in the control group, whereas a decrease in the level of iNOS was found in the L-carnitine treated group. In the present study, we found that bile duct ligation in rats showed an increase in body and liver weight, while treatment with carnitine showed normal body and liver weight. Serum AST, ALT, total bilirubin, HP, GSH, MDA, and cytokines were increased in bile duct ligated rats. In addition, L-carnitine treated rats showed a reduction in oxidative stress as well as inhibiting the release of cytokines in a dose-dependent manner and showed protection against bile duct ligation. The study concludes that L-carnitine has a protective effect against the liver fibrosis induced by bile duct ligation.
format	article
author	Vikram Nimbalkar Neeraj Vyawahare
author_facet	Vikram Nimbalkar Neeraj Vyawahare
author_sort	Vikram Nimbalkar
title	L-carnitine ameliorates bile duct ligation induced liver fibrosis via reducing the nitrosative stress in experimental animals: preclinical evidences
title_short	L-carnitine ameliorates bile duct ligation induced liver fibrosis via reducing the nitrosative stress in experimental animals: preclinical evidences
title_full	L-carnitine ameliorates bile duct ligation induced liver fibrosis via reducing the nitrosative stress in experimental animals: preclinical evidences
title_fullStr	L-carnitine ameliorates bile duct ligation induced liver fibrosis via reducing the nitrosative stress in experimental animals: preclinical evidences
title_full_unstemmed	L-carnitine ameliorates bile duct ligation induced liver fibrosis via reducing the nitrosative stress in experimental animals: preclinical evidences
title_sort	l-carnitine ameliorates bile duct ligation induced liver fibrosis via reducing the nitrosative stress in experimental animals: preclinical evidences
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/f65d7698b7fd4fc1adb84b9940aeb435
work_keys_str_mv	AT vikramnimbalkar lcarnitineamelioratesbileductligationinducedliverfibrosisviareducingthenitrosativestressinexperimentalanimalspreclinicalevidences AT neerajvyawahare lcarnitineamelioratesbileductligationinducedliverfibrosisviareducingthenitrosativestressinexperimentalanimalspreclinicalevidences
_version_	1718400748474073088

L-carnitine ameliorates bile duct ligation induced liver fibrosis via reducing the nitrosative stress in experimental animals: preclinical evidences

Ejemplares similares