PEDOT:PSS-based Multilayer Bacterial-Composite Films for Bioelectronics

Abstract Microbial electrochemical systems provide an environmentally-friendly means of energy conversion between chemical and electrical forms, with applications in wastewater treatment, bioelectronics, and biosensing. However, a major challenge to further development, miniaturization, and deployme...

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Autores principales:	Tom J. Zajdel, Moshe Baruch, Gábor Méhes, Eleni Stavrinidou, Magnus Berggren, Michel M. Maharbiz, Daniel T. Simon, Caroline M. Ajo-Franklin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Microbial Electrochemical Systems (MESs) Oneidensis Electroactive Bacteria Natural Biofilms Carbon Fiber Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f6602354c3104a48bbe0e15119be24e6
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spelling	oai:doaj.org-article:f6602354c3104a48bbe0e15119be24e62021-12-02T15:08:17ZPEDOT:PSS-based Multilayer Bacterial-Composite Films for Bioelectronics10.1038/s41598-018-33521-92045-2322https://doaj.org/article/f6602354c3104a48bbe0e15119be24e62018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33521-9https://doaj.org/toc/2045-2322Abstract Microbial electrochemical systems provide an environmentally-friendly means of energy conversion between chemical and electrical forms, with applications in wastewater treatment, bioelectronics, and biosensing. However, a major challenge to further development, miniaturization, and deployment of bioelectronics and biosensors is the limited thickness of biofilms, necessitating large anodes to achieve sufficient signal-to-noise ratios. Here we demonstrate a method for embedding an electroactive bacterium, Shewanella oneidensis MR-1, inside a conductive three-dimensional poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) matrix electropolymerized on a carbon felt substrate, which we call a multilayer conductive bacterial-composite film (MCBF). By mixing the bacteria with the PEDOT:PSS precursor in a flow-through method, we maintain over 90% viability of S. oneidensis during encapsulation. Microscopic analysis of the MCBFs reveal a tightly interleaved structure of bacteria and conductive PEDOT:PSS up to 80 µm thick. Electrochemical experiments indicate S. oneidensis in MCBFs can perform both direct and riboflavin-mediated electron transfer to PEDOT:PSS. When used in bioelectrochemical reactors, the MCBFs produce 20 times more steady-state current than native biofilms grown on unmodified carbon felt. This versatile approach to control the thickness of bacterial composite films and increase their current output has immediate applications in microbial electrochemical systems, including field-deployable environmental sensing and direct integration of microorganisms into miniaturized organic electronics.Tom J. ZajdelMoshe BaruchGábor MéhesEleni StavrinidouMagnus BerggrenMichel M. MaharbizDaniel T. SimonCaroline M. Ajo-FranklinNature PortfolioarticleMicrobial Electrochemical Systems (MESs)OneidensisElectroactive BacteriaNatural BiofilmsCarbon FiberMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial Electrochemical Systems (MESs) Oneidensis Electroactive Bacteria Natural Biofilms Carbon Fiber Medicine R Science Q
spellingShingle	Microbial Electrochemical Systems (MESs) Oneidensis Electroactive Bacteria Natural Biofilms Carbon Fiber Medicine R Science Q Tom J. Zajdel Moshe Baruch Gábor Méhes Eleni Stavrinidou Magnus Berggren Michel M. Maharbiz Daniel T. Simon Caroline M. Ajo-Franklin PEDOT:PSS-based Multilayer Bacterial-Composite Films for Bioelectronics
description	Abstract Microbial electrochemical systems provide an environmentally-friendly means of energy conversion between chemical and electrical forms, with applications in wastewater treatment, bioelectronics, and biosensing. However, a major challenge to further development, miniaturization, and deployment of bioelectronics and biosensors is the limited thickness of biofilms, necessitating large anodes to achieve sufficient signal-to-noise ratios. Here we demonstrate a method for embedding an electroactive bacterium, Shewanella oneidensis MR-1, inside a conductive three-dimensional poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) matrix electropolymerized on a carbon felt substrate, which we call a multilayer conductive bacterial-composite film (MCBF). By mixing the bacteria with the PEDOT:PSS precursor in a flow-through method, we maintain over 90% viability of S. oneidensis during encapsulation. Microscopic analysis of the MCBFs reveal a tightly interleaved structure of bacteria and conductive PEDOT:PSS up to 80 µm thick. Electrochemical experiments indicate S. oneidensis in MCBFs can perform both direct and riboflavin-mediated electron transfer to PEDOT:PSS. When used in bioelectrochemical reactors, the MCBFs produce 20 times more steady-state current than native biofilms grown on unmodified carbon felt. This versatile approach to control the thickness of bacterial composite films and increase their current output has immediate applications in microbial electrochemical systems, including field-deployable environmental sensing and direct integration of microorganisms into miniaturized organic electronics.
format	article
author	Tom J. Zajdel Moshe Baruch Gábor Méhes Eleni Stavrinidou Magnus Berggren Michel M. Maharbiz Daniel T. Simon Caroline M. Ajo-Franklin
author_facet	Tom J. Zajdel Moshe Baruch Gábor Méhes Eleni Stavrinidou Magnus Berggren Michel M. Maharbiz Daniel T. Simon Caroline M. Ajo-Franklin
author_sort	Tom J. Zajdel
title	PEDOT:PSS-based Multilayer Bacterial-Composite Films for Bioelectronics
title_short	PEDOT:PSS-based Multilayer Bacterial-Composite Films for Bioelectronics
title_full	PEDOT:PSS-based Multilayer Bacterial-Composite Films for Bioelectronics
title_fullStr	PEDOT:PSS-based Multilayer Bacterial-Composite Films for Bioelectronics
title_full_unstemmed	PEDOT:PSS-based Multilayer Bacterial-Composite Films for Bioelectronics
title_sort	pedot:pss-based multilayer bacterial-composite films for bioelectronics
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/f6602354c3104a48bbe0e15119be24e6
work_keys_str_mv	AT tomjzajdel pedotpssbasedmultilayerbacterialcompositefilmsforbioelectronics AT moshebaruch pedotpssbasedmultilayerbacterialcompositefilmsforbioelectronics AT gabormehes pedotpssbasedmultilayerbacterialcompositefilmsforbioelectronics AT elenistavrinidou pedotpssbasedmultilayerbacterialcompositefilmsforbioelectronics AT magnusberggren pedotpssbasedmultilayerbacterialcompositefilmsforbioelectronics AT michelmmaharbiz pedotpssbasedmultilayerbacterialcompositefilmsforbioelectronics AT danieltsimon pedotpssbasedmultilayerbacterialcompositefilmsforbioelectronics AT carolinemajofranklin pedotpssbasedmultilayerbacterialcompositefilmsforbioelectronics
_version_	1718388228602462208

PEDOT:PSS-based Multilayer Bacterial-Composite Films for Bioelectronics

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