Latest Advances in Protein-Recovery Technologies from Agricultural Waste

In recent years, downstream bioprocessing industries are venturing into less tedious, simple, and high-efficiency separation by implementing advanced purification and extraction methods. This review discusses the separation of proteins, with the main focus on amylase as an enzyme from agricultural w...

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Autores principales:	Farhana Iylia Fatinee Mohd Yusree, Angela Paul Peter, Mohd Zuhair Mohd Nor, Pau Loke Show, Mohd Noriznan Mokhtar
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	agricultural waste protein amylase liquid biphasic system separation Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/f66482f10d464be7883736b2bd4f468c
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spelling	oai:doaj.org-article:f66482f10d464be7883736b2bd4f468c2021-11-25T17:35:09ZLatest Advances in Protein-Recovery Technologies from Agricultural Waste10.3390/foods101127482304-8158https://doaj.org/article/f66482f10d464be7883736b2bd4f468c2021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2748https://doaj.org/toc/2304-8158In recent years, downstream bioprocessing industries are venturing into less tedious, simple, and high-efficiency separation by implementing advanced purification and extraction methods. This review discusses the separation of proteins, with the main focus on amylase as an enzyme from agricultural waste using conventional and advanced techniques of extraction and purification via a liquid biphasic system (LBS). In comparison to other methods, such as membrane extraction, precipitation, ultrasonication, and chromatography, the LBS stands out as an efficient, cost-effective, and adaptable developing method for protein recovery. The two-phase separation method can be water-soluble polymers, or polymer and salt, or alcohol and salt, which is a simpler and lower-cost method that can be used at a larger purification scale. The comparison of different approaches in LBS for amylase purification from agricultural waste is also included. Current technology has evolved from a simple LBS into microwave-assisted LBS, liquid biphasic flotation (LBF), thermoseparation (TMP), three-phase partitioning (TPP), ultrasound-assisted LBS, and electrically assisted LBS. pH, time, temperature, and concentration are some of the significant research parameters considered in the review of advanced techniques.Farhana Iylia Fatinee Mohd YusreeAngela Paul PeterMohd Zuhair Mohd NorPau Loke ShowMohd Noriznan MokhtarMDPI AGarticleagricultural wasteproteinamylaseliquid biphasic systemseparationChemical technologyTP1-1185ENFoods, Vol 10, Iss 2748, p 2748 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	agricultural waste protein amylase liquid biphasic system separation Chemical technology TP1-1185
spellingShingle	agricultural waste protein amylase liquid biphasic system separation Chemical technology TP1-1185 Farhana Iylia Fatinee Mohd Yusree Angela Paul Peter Mohd Zuhair Mohd Nor Pau Loke Show Mohd Noriznan Mokhtar Latest Advances in Protein-Recovery Technologies from Agricultural Waste
description	In recent years, downstream bioprocessing industries are venturing into less tedious, simple, and high-efficiency separation by implementing advanced purification and extraction methods. This review discusses the separation of proteins, with the main focus on amylase as an enzyme from agricultural waste using conventional and advanced techniques of extraction and purification via a liquid biphasic system (LBS). In comparison to other methods, such as membrane extraction, precipitation, ultrasonication, and chromatography, the LBS stands out as an efficient, cost-effective, and adaptable developing method for protein recovery. The two-phase separation method can be water-soluble polymers, or polymer and salt, or alcohol and salt, which is a simpler and lower-cost method that can be used at a larger purification scale. The comparison of different approaches in LBS for amylase purification from agricultural waste is also included. Current technology has evolved from a simple LBS into microwave-assisted LBS, liquid biphasic flotation (LBF), thermoseparation (TMP), three-phase partitioning (TPP), ultrasound-assisted LBS, and electrically assisted LBS. pH, time, temperature, and concentration are some of the significant research parameters considered in the review of advanced techniques.
format	article
author	Farhana Iylia Fatinee Mohd Yusree Angela Paul Peter Mohd Zuhair Mohd Nor Pau Loke Show Mohd Noriznan Mokhtar
author_facet	Farhana Iylia Fatinee Mohd Yusree Angela Paul Peter Mohd Zuhair Mohd Nor Pau Loke Show Mohd Noriznan Mokhtar
author_sort	Farhana Iylia Fatinee Mohd Yusree
title	Latest Advances in Protein-Recovery Technologies from Agricultural Waste
title_short	Latest Advances in Protein-Recovery Technologies from Agricultural Waste
title_full	Latest Advances in Protein-Recovery Technologies from Agricultural Waste
title_fullStr	Latest Advances in Protein-Recovery Technologies from Agricultural Waste
title_full_unstemmed	Latest Advances in Protein-Recovery Technologies from Agricultural Waste
title_sort	latest advances in protein-recovery technologies from agricultural waste
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f66482f10d464be7883736b2bd4f468c
work_keys_str_mv	AT farhanaiyliafatineemohdyusree latestadvancesinproteinrecoverytechnologiesfromagriculturalwaste AT angelapaulpeter latestadvancesinproteinrecoverytechnologiesfromagriculturalwaste AT mohdzuhairmohdnor latestadvancesinproteinrecoverytechnologiesfromagriculturalwaste AT paulokeshow latestadvancesinproteinrecoverytechnologiesfromagriculturalwaste AT mohdnoriznanmokhtar latestadvancesinproteinrecoverytechnologiesfromagriculturalwaste
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Latest Advances in Protein-Recovery Technologies from Agricultural Waste

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