First Global Report of Plasmid-Mediated <i>mcr-1</i> and Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> from Sheep in Portugal

Resistances to extended-spectrum cephalosporins (ESC) and colistin are One Health issues since genes encoding these resistances can be transmitted between all sectors of the One Health concept, i.e., human, animal, and the environment. Among food-producing animals, sheep farming has long been overlo...

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Autores principales:	Josman Dantas Palmeira, Marisa Haenni, Jean-Yves Madec, Helena Maria Neto Ferreira
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>mcr-1</i> extended-spectrum beta-lactamase <i>E. coli</i> sheep Portugal Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/f66d96e728564ea8a99e2b19272f96bb
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spelling	oai:doaj.org-article:f66d96e728564ea8a99e2b19272f96bb2021-11-25T16:24:50ZFirst Global Report of Plasmid-Mediated <i>mcr-1</i> and Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> from Sheep in Portugal10.3390/antibiotics101114032079-6382https://doaj.org/article/f66d96e728564ea8a99e2b19272f96bb2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1403https://doaj.org/toc/2079-6382Resistances to extended-spectrum cephalosporins (ESC) and colistin are One Health issues since genes encoding these resistances can be transmitted between all sectors of the One Health concept, i.e., human, animal, and the environment. Among food-producing animals, sheep farming has long been overlooked. To fill in this knowledge gap, we looked for ESC- and colistin resistance in 21 faecal samples collected from sheep in one farm in the south of Portugal. ESC-resistant isolates were selected on MacConkey agar plates supplemented with cefotaxime. Susceptibility testing was performed by the disk-diffusion method according to CLSI, while colistin MIC was determined by broth microdilution. ESC- and colistin-resistance genes were identified by PCR, and the clonality of all isolates was assessed by <i>XbaI</i>-PFGE. The replicon content was determined by PCR according to the PCR-based replicon typing (PBRT) scheme. Sixty-two non-duplicate ESC-resistant <i>E. coli</i> isolates were identified, which all presented an extended-spectrum beta-lactamase (ESBL) phenotype, mostly due to the presence of CTX-M genes. One CTX-M-1-producing <i>E. coli</i> was concomitantly colistin-resistant and presented the plasmid-mediated <i>mcr-1</i> gene. Nearly all isolates showed associated resistances to non-beta-lactam antibiotics, which could act as co-selectors, even in the absence of beta-lactam use. The results showed a high proportion of ESBL-producing <i>E. coli</i> in sheep faeces. Their dissemination was very dynamic, with the spread of successful clones between animals, but also a large diversity of clones and plasmids, sometimes residing in the same animal. This study highlights the need for global surveillance in all food-producing sectors, in order to avoid the dissemination of genes conferring resistance to last-resort antibiotics in human medicine.Josman Dantas PalmeiraMarisa HaenniJean-Yves MadecHelena Maria Neto FerreiraMDPI AGarticle<i>mcr-1</i>extended-spectrum beta-lactamase<i>E. coli</i>sheepPortugalTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1403, p 1403 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>mcr-1</i> extended-spectrum beta-lactamase <i>E. coli</i> sheep Portugal Therapeutics. Pharmacology RM1-950
spellingShingle	<i>mcr-1</i> extended-spectrum beta-lactamase <i>E. coli</i> sheep Portugal Therapeutics. Pharmacology RM1-950 Josman Dantas Palmeira Marisa Haenni Jean-Yves Madec Helena Maria Neto Ferreira First Global Report of Plasmid-Mediated <i>mcr-1</i> and Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> from Sheep in Portugal
description	Resistances to extended-spectrum cephalosporins (ESC) and colistin are One Health issues since genes encoding these resistances can be transmitted between all sectors of the One Health concept, i.e., human, animal, and the environment. Among food-producing animals, sheep farming has long been overlooked. To fill in this knowledge gap, we looked for ESC- and colistin resistance in 21 faecal samples collected from sheep in one farm in the south of Portugal. ESC-resistant isolates were selected on MacConkey agar plates supplemented with cefotaxime. Susceptibility testing was performed by the disk-diffusion method according to CLSI, while colistin MIC was determined by broth microdilution. ESC- and colistin-resistance genes were identified by PCR, and the clonality of all isolates was assessed by <i>XbaI</i>-PFGE. The replicon content was determined by PCR according to the PCR-based replicon typing (PBRT) scheme. Sixty-two non-duplicate ESC-resistant <i>E. coli</i> isolates were identified, which all presented an extended-spectrum beta-lactamase (ESBL) phenotype, mostly due to the presence of CTX-M genes. One CTX-M-1-producing <i>E. coli</i> was concomitantly colistin-resistant and presented the plasmid-mediated <i>mcr-1</i> gene. Nearly all isolates showed associated resistances to non-beta-lactam antibiotics, which could act as co-selectors, even in the absence of beta-lactam use. The results showed a high proportion of ESBL-producing <i>E. coli</i> in sheep faeces. Their dissemination was very dynamic, with the spread of successful clones between animals, but also a large diversity of clones and plasmids, sometimes residing in the same animal. This study highlights the need for global surveillance in all food-producing sectors, in order to avoid the dissemination of genes conferring resistance to last-resort antibiotics in human medicine.
format	article
author	Josman Dantas Palmeira Marisa Haenni Jean-Yves Madec Helena Maria Neto Ferreira
author_facet	Josman Dantas Palmeira Marisa Haenni Jean-Yves Madec Helena Maria Neto Ferreira
author_sort	Josman Dantas Palmeira
title	First Global Report of Plasmid-Mediated <i>mcr-1</i> and Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> from Sheep in Portugal
title_short	First Global Report of Plasmid-Mediated <i>mcr-1</i> and Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> from Sheep in Portugal
title_full	First Global Report of Plasmid-Mediated <i>mcr-1</i> and Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> from Sheep in Portugal
title_fullStr	First Global Report of Plasmid-Mediated <i>mcr-1</i> and Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> from Sheep in Portugal
title_full_unstemmed	First Global Report of Plasmid-Mediated <i>mcr-1</i> and Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> from Sheep in Portugal
title_sort	first global report of plasmid-mediated <i>mcr-1</i> and extended-spectrum beta-lactamase-producing <i>escherichia coli</i> from sheep in portugal
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f66d96e728564ea8a99e2b19272f96bb
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First Global Report of Plasmid-Mediated <i>mcr-1</i> and Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> from Sheep in Portugal

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