Circular RNA CNOT2 knockdown regulates twist family BHLH transcription factor via targeting microRNA 409-3p to prevent breast cancer invasion, migration and epithelial–mesenchymal transition

Numerous studies have manifested that cicular RNA (circRNA) is closely associated with the development of breast cancer (BC), but the specific mechanism has not been fully clarified. The purpose of this study was to investigate the effect of circCNOT2 on BC invasion, migration and epithelial mesench...

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Autores principales:	QunXue Su, Hao Shen, Bei Gu, Ning Zhu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	circularrna cnot2 microrna-409-3p twist family bhlh transcription factor breast cancer epithelial–mesenchymal transition Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/f66f72aa0a65471c814c12d8c80b7d88
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spelling	oai:doaj.org-article:f66f72aa0a65471c814c12d8c80b7d882021-11-04T15:51:53ZCircular RNA CNOT2 knockdown regulates twist family BHLH transcription factor via targeting microRNA 409-3p to prevent breast cancer invasion, migration and epithelial–mesenchymal transition2165-59792165-598710.1080/21655979.2021.1974805https://doaj.org/article/f66f72aa0a65471c814c12d8c80b7d882021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1974805https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Numerous studies have manifested that cicular RNA (circRNA) is closely associated with the development of breast cancer (BC), but the specific mechanism has not been fully clarified. The purpose of this study was to investigate the effect of circCNOT2 on BC invasion, migration and epithelial mesenchymal transition (EMT) and its potential molecular mechanism. The results assured that circCNOT2 and Twist Family BHLH Transcription Factor (TWIST1) were elevated in BC, while microrNA (miR)-409-3p was reduced. CircCNOT2 was positively correlated with TWIST1 and negatively correlated with miR-409-3p. Elevated circCNOT2 is associated with poor prognosis of BC. Knockdown circCNOT2 or augmented miR-409-3p could promote apoptosis but repress proliferation, invasion, migration and EMT of BC cells. In addition, overexpression of circCNOT2 or TWIST1 accelerated BC invasion, migration and EMT, which could be reversed by simultaneous transfection of miR-409-3p-mimic. Further dual luciferase reporting and RNA-pull down assay clarified that circCNOT2 acted as a competing endogenous RNA of miR-409-3p to mediate TWIST1 expression. In conclusion, the results of this study suggest that circCNOT2 affects the biological behavior of BC via regulating the miR-409-3p/TWIST1 axis, and may be applied as a potential therapeutic target for BC later.QunXue SuHao ShenBei GuNing ZhuTaylor & Francis Grouparticlecircularrna cnot2microrna-409-3ptwist family bhlh transcription factorbreast cancerepithelial–mesenchymal transitionBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 9058-9069 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circularrna cnot2 microrna-409-3p twist family bhlh transcription factor breast cancer epithelial–mesenchymal transition Biotechnology TP248.13-248.65
spellingShingle	circularrna cnot2 microrna-409-3p twist family bhlh transcription factor breast cancer epithelial–mesenchymal transition Biotechnology TP248.13-248.65 QunXue Su Hao Shen Bei Gu Ning Zhu Circular RNA CNOT2 knockdown regulates twist family BHLH transcription factor via targeting microRNA 409-3p to prevent breast cancer invasion, migration and epithelial–mesenchymal transition
description	Numerous studies have manifested that cicular RNA (circRNA) is closely associated with the development of breast cancer (BC), but the specific mechanism has not been fully clarified. The purpose of this study was to investigate the effect of circCNOT2 on BC invasion, migration and epithelial mesenchymal transition (EMT) and its potential molecular mechanism. The results assured that circCNOT2 and Twist Family BHLH Transcription Factor (TWIST1) were elevated in BC, while microrNA (miR)-409-3p was reduced. CircCNOT2 was positively correlated with TWIST1 and negatively correlated with miR-409-3p. Elevated circCNOT2 is associated with poor prognosis of BC. Knockdown circCNOT2 or augmented miR-409-3p could promote apoptosis but repress proliferation, invasion, migration and EMT of BC cells. In addition, overexpression of circCNOT2 or TWIST1 accelerated BC invasion, migration and EMT, which could be reversed by simultaneous transfection of miR-409-3p-mimic. Further dual luciferase reporting and RNA-pull down assay clarified that circCNOT2 acted as a competing endogenous RNA of miR-409-3p to mediate TWIST1 expression. In conclusion, the results of this study suggest that circCNOT2 affects the biological behavior of BC via regulating the miR-409-3p/TWIST1 axis, and may be applied as a potential therapeutic target for BC later.
format	article
author	QunXue Su Hao Shen Bei Gu Ning Zhu
author_facet	QunXue Su Hao Shen Bei Gu Ning Zhu
author_sort	QunXue Su
title	Circular RNA CNOT2 knockdown regulates twist family BHLH transcription factor via targeting microRNA 409-3p to prevent breast cancer invasion, migration and epithelial–mesenchymal transition
title_short	Circular RNA CNOT2 knockdown regulates twist family BHLH transcription factor via targeting microRNA 409-3p to prevent breast cancer invasion, migration and epithelial–mesenchymal transition
title_full	Circular RNA CNOT2 knockdown regulates twist family BHLH transcription factor via targeting microRNA 409-3p to prevent breast cancer invasion, migration and epithelial–mesenchymal transition
title_fullStr	Circular RNA CNOT2 knockdown regulates twist family BHLH transcription factor via targeting microRNA 409-3p to prevent breast cancer invasion, migration and epithelial–mesenchymal transition
title_full_unstemmed	Circular RNA CNOT2 knockdown regulates twist family BHLH transcription factor via targeting microRNA 409-3p to prevent breast cancer invasion, migration and epithelial–mesenchymal transition
title_sort	circular rna cnot2 knockdown regulates twist family bhlh transcription factor via targeting microrna 409-3p to prevent breast cancer invasion, migration and epithelial–mesenchymal transition
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/f66f72aa0a65471c814c12d8c80b7d88
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Circular RNA CNOT2 knockdown regulates twist family BHLH transcription factor via targeting microRNA 409-3p to prevent breast cancer invasion, migration and epithelial–mesenchymal transition

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