Genome sequence of Lactobacillus pentosus KCA1: vaginal isolate from a healthy premenopausal woman.

The vaginal microbiota, in particular Lactobacillus species, play an important role in female health through modulation of immunity, countering pathogens and maintaining a pH below 4.7. We report the isolation and genome sequence of Lactobacillus pentosus strain KCA1 (formally known as L. plantarum)...

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Autores principales:	Kingsley C Anukam, Jean M Macklaim, Gregory B Gloor, Gregor Reid, Jos Boekhorst, Bernadet Renckens, Sacha A F T van Hijum, Roland J Siezen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f6879878d3d2457f839a434a39f52ecf
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spelling	oai:doaj.org-article:f6879878d3d2457f839a434a39f52ecf2021-11-18T07:52:53ZGenome sequence of Lactobacillus pentosus KCA1: vaginal isolate from a healthy premenopausal woman.1932-620310.1371/journal.pone.0059239https://doaj.org/article/f6879878d3d2457f839a434a39f52ecf2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23527145/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The vaginal microbiota, in particular Lactobacillus species, play an important role in female health through modulation of immunity, countering pathogens and maintaining a pH below 4.7. We report the isolation and genome sequence of Lactobacillus pentosus strain KCA1 (formally known as L. plantarum) from the vagina of a healthy Nigerian woman. The genome was sequenced using Illumina GA II technology. The resulting 16,920,226 paired-end reads were assembled with the Velvet tool. Contigs were annotated using the RAST server, and manually curated. A comparative analysis with the available genomes of L. pentosus IG1 and L. plantarum WCFS1 showed that over 15% of the predicted functional activities are found only in this strain. The strain has a chromosome sequence of 3,418,159 bp with a G+C content of 46.4%, and is devoid of plasmids. Novel gene clusters or variants of known genes relative to the reference genomes were found. In particular, the strain has loci encoding additional putative mannose phosphotransferase systems. Clusters of genes include those for utilization of hydantoin, isopropylmalate, malonate, rhamnosides, and genes for assimilation of polyglycans, suggesting the metabolic versatility of L. pentosus KCA1. Loci encoding putative phage defense systems were also found including clustered regularly interspaced short palindromic repeats (CRISPRs), abortive infection (Abi) systems and toxin-antitoxin systems (TA). A putative cluster of genes for biosynthesis of a cyclic bacteriocin precursor, here designated as pentocin KCA1 (penA) were identified. These findings add crucial information for understanding the genomic and geographic diversity of vaginal lactobacilli.Kingsley C AnukamJean M MacklaimGregory B GloorGregor ReidJos BoekhorstBernadet RenckensSacha A F T van HijumRoland J SiezenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e59239 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kingsley C Anukam Jean M Macklaim Gregory B Gloor Gregor Reid Jos Boekhorst Bernadet Renckens Sacha A F T van Hijum Roland J Siezen Genome sequence of Lactobacillus pentosus KCA1: vaginal isolate from a healthy premenopausal woman.
description	The vaginal microbiota, in particular Lactobacillus species, play an important role in female health through modulation of immunity, countering pathogens and maintaining a pH below 4.7. We report the isolation and genome sequence of Lactobacillus pentosus strain KCA1 (formally known as L. plantarum) from the vagina of a healthy Nigerian woman. The genome was sequenced using Illumina GA II technology. The resulting 16,920,226 paired-end reads were assembled with the Velvet tool. Contigs were annotated using the RAST server, and manually curated. A comparative analysis with the available genomes of L. pentosus IG1 and L. plantarum WCFS1 showed that over 15% of the predicted functional activities are found only in this strain. The strain has a chromosome sequence of 3,418,159 bp with a G+C content of 46.4%, and is devoid of plasmids. Novel gene clusters or variants of known genes relative to the reference genomes were found. In particular, the strain has loci encoding additional putative mannose phosphotransferase systems. Clusters of genes include those for utilization of hydantoin, isopropylmalate, malonate, rhamnosides, and genes for assimilation of polyglycans, suggesting the metabolic versatility of L. pentosus KCA1. Loci encoding putative phage defense systems were also found including clustered regularly interspaced short palindromic repeats (CRISPRs), abortive infection (Abi) systems and toxin-antitoxin systems (TA). A putative cluster of genes for biosynthesis of a cyclic bacteriocin precursor, here designated as pentocin KCA1 (penA) were identified. These findings add crucial information for understanding the genomic and geographic diversity of vaginal lactobacilli.
format	article
author	Kingsley C Anukam Jean M Macklaim Gregory B Gloor Gregor Reid Jos Boekhorst Bernadet Renckens Sacha A F T van Hijum Roland J Siezen
author_facet	Kingsley C Anukam Jean M Macklaim Gregory B Gloor Gregor Reid Jos Boekhorst Bernadet Renckens Sacha A F T van Hijum Roland J Siezen
author_sort	Kingsley C Anukam
title	Genome sequence of Lactobacillus pentosus KCA1: vaginal isolate from a healthy premenopausal woman.
title_short	Genome sequence of Lactobacillus pentosus KCA1: vaginal isolate from a healthy premenopausal woman.
title_full	Genome sequence of Lactobacillus pentosus KCA1: vaginal isolate from a healthy premenopausal woman.
title_fullStr	Genome sequence of Lactobacillus pentosus KCA1: vaginal isolate from a healthy premenopausal woman.
title_full_unstemmed	Genome sequence of Lactobacillus pentosus KCA1: vaginal isolate from a healthy premenopausal woman.
title_sort	genome sequence of lactobacillus pentosus kca1: vaginal isolate from a healthy premenopausal woman.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f6879878d3d2457f839a434a39f52ecf
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Genome sequence of Lactobacillus pentosus KCA1: vaginal isolate from a healthy premenopausal woman.

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