NEPHRITOGENIC ACTIVITY OF STREPTOCOCCUS PYOGENES emm1 AND emm12 GENOTYPES ISOLATED FROM PATIENTS AND ASYMPTOMATIC CARRIERS

In this paper the nephritogenic activity of Streptococcus pyogenes genotype emm1 and emm12 clinical isolates from scarlet fever patients and healthy children was considered. As earlier established, strains of these types differ in Fc-binding profile, interacting with native IgG and immune complexes...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	L. A. Burova, P. V. Pigarevsky, V. A. Snegova, E. V. Kuleshevich, D. A. Zharkov, C. Schalen, Artem A. Totolian
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2015
Materias:	postinfectious glomerulonephritis streptococcus pyogenes fc-reception igg immune complexes Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/f68f515081c04d7db9e21492f57d1e59
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f68f515081c04d7db9e21492f57d1e59
record_format	dspace
spelling	oai:doaj.org-article:f68f515081c04d7db9e21492f57d1e592021-11-22T07:09:47ZNEPHRITOGENIC ACTIVITY OF STREPTOCOCCUS PYOGENES emm1 AND emm12 GENOTYPES ISOLATED FROM PATIENTS AND ASYMPTOMATIC CARRIERS2220-76192313-739810.15789/2220-7619-2015-3-233-242https://doaj.org/article/f68f515081c04d7db9e21492f57d1e592015-12-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/328https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398In this paper the nephritogenic activity of Streptococcus pyogenes genotype emm1 and emm12 clinical isolates from scarlet fever patients and healthy children was considered. As earlier established, strains of these types differ in Fc-binding profile, interacting with native IgG and immune complexes (IC), respectively. As expected, all the type emm1 strains bound native IgG; besides, ICs interacted only with strains from patients but not with those from carriers. In contrast, all type emm12 strains appeared to be negative for native IgG, whereas ICs were bound by strains from patients exclusively. None of the tested strains bound IgG3. By immunization of rabbits, binding of native IgG as well as ICs was associated with increasing of anti-IgG antibodies titer, formation of ICs, «crescent» deposition of IgG and C3-complement, local production of the proinflammatory cytokine TNFα, аnd also with accumulation of lymphocytes in kidney tissue. These signs indicated immune inflammation, leading to experimental membrane-proliferative glomerulonephritis (PSGN). It is known that PSGN development depends on IC-binding by tissue FcγR, on complement activation as well as on tissue infiltration by macrophages/monocytes. According to the data of morphometric evaluation the nephritogenic activity of the type emm12 strains exceeded those of type emm1. On testing of three IC-binding emm12 strains in six rabbits, typical PSGN developed in 5 of them and an abortive process in 1 animal. In case of five IgG-binding type emm1 strains, out of ten rabbits full-blown PSGN was observed only in 3 of them, but abortive changes in 5 and negative result in 2 animals. No pathologic changes were elicited by the «carrier» strains of either genotype; the inability of these to bind ICs, according to literature data, could be explained by mutation in the Mga-regulator gene thereby impeding M-proteins synthesis. We conclude that isolation of type emm12 IC-binding strains at acute streptococcal infection should be considered a high risk-factor for postinfectious sequelae development. The rabbit model of PSGN used in this research thus allowed to reveal some main stages and features of its pathogenesis.L. A. BurovaP. V. PigarevskyV. A. SnegovaE. V. KuleshevichD. A. ZharkovC. SchalenArtem A. TotolianSankt-Peterburg : NIIÈM imeni Pasteraarticlepostinfectious glomerulonephritisstreptococcus pyogenesfc-receptioniggimmune complexesInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 5, Iss 3, Pp 233-242 (2015)
institution	DOAJ
collection	DOAJ
language	RU
topic	postinfectious glomerulonephritis streptococcus pyogenes fc-reception igg immune complexes Infectious and parasitic diseases RC109-216
spellingShingle	postinfectious glomerulonephritis streptococcus pyogenes fc-reception igg immune complexes Infectious and parasitic diseases RC109-216 L. A. Burova P. V. Pigarevsky V. A. Snegova E. V. Kuleshevich D. A. Zharkov C. Schalen Artem A. Totolian NEPHRITOGENIC ACTIVITY OF STREPTOCOCCUS PYOGENES emm1 AND emm12 GENOTYPES ISOLATED FROM PATIENTS AND ASYMPTOMATIC CARRIERS
description	In this paper the nephritogenic activity of Streptococcus pyogenes genotype emm1 and emm12 clinical isolates from scarlet fever patients and healthy children was considered. As earlier established, strains of these types differ in Fc-binding profile, interacting with native IgG and immune complexes (IC), respectively. As expected, all the type emm1 strains bound native IgG; besides, ICs interacted only with strains from patients but not with those from carriers. In contrast, all type emm12 strains appeared to be negative for native IgG, whereas ICs were bound by strains from patients exclusively. None of the tested strains bound IgG3. By immunization of rabbits, binding of native IgG as well as ICs was associated with increasing of anti-IgG antibodies titer, formation of ICs, «crescent» deposition of IgG and C3-complement, local production of the proinflammatory cytokine TNFα, аnd also with accumulation of lymphocytes in kidney tissue. These signs indicated immune inflammation, leading to experimental membrane-proliferative glomerulonephritis (PSGN). It is known that PSGN development depends on IC-binding by tissue FcγR, on complement activation as well as on tissue infiltration by macrophages/monocytes. According to the data of morphometric evaluation the nephritogenic activity of the type emm12 strains exceeded those of type emm1. On testing of three IC-binding emm12 strains in six rabbits, typical PSGN developed in 5 of them and an abortive process in 1 animal. In case of five IgG-binding type emm1 strains, out of ten rabbits full-blown PSGN was observed only in 3 of them, but abortive changes in 5 and negative result in 2 animals. No pathologic changes were elicited by the «carrier» strains of either genotype; the inability of these to bind ICs, according to literature data, could be explained by mutation in the Mga-regulator gene thereby impeding M-proteins synthesis. We conclude that isolation of type emm12 IC-binding strains at acute streptococcal infection should be considered a high risk-factor for postinfectious sequelae development. The rabbit model of PSGN used in this research thus allowed to reveal some main stages and features of its pathogenesis.
format	article
author	L. A. Burova P. V. Pigarevsky V. A. Snegova E. V. Kuleshevich D. A. Zharkov C. Schalen Artem A. Totolian
author_facet	L. A. Burova P. V. Pigarevsky V. A. Snegova E. V. Kuleshevich D. A. Zharkov C. Schalen Artem A. Totolian
author_sort	L. A. Burova
title	NEPHRITOGENIC ACTIVITY OF STREPTOCOCCUS PYOGENES emm1 AND emm12 GENOTYPES ISOLATED FROM PATIENTS AND ASYMPTOMATIC CARRIERS
title_short	NEPHRITOGENIC ACTIVITY OF STREPTOCOCCUS PYOGENES emm1 AND emm12 GENOTYPES ISOLATED FROM PATIENTS AND ASYMPTOMATIC CARRIERS
title_full	NEPHRITOGENIC ACTIVITY OF STREPTOCOCCUS PYOGENES emm1 AND emm12 GENOTYPES ISOLATED FROM PATIENTS AND ASYMPTOMATIC CARRIERS
title_fullStr	NEPHRITOGENIC ACTIVITY OF STREPTOCOCCUS PYOGENES emm1 AND emm12 GENOTYPES ISOLATED FROM PATIENTS AND ASYMPTOMATIC CARRIERS
title_full_unstemmed	NEPHRITOGENIC ACTIVITY OF STREPTOCOCCUS PYOGENES emm1 AND emm12 GENOTYPES ISOLATED FROM PATIENTS AND ASYMPTOMATIC CARRIERS
title_sort	nephritogenic activity of streptococcus pyogenes emm1 and emm12 genotypes isolated from patients and asymptomatic carriers
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2015
url	https://doaj.org/article/f68f515081c04d7db9e21492f57d1e59
work_keys_str_mv	AT laburova nephritogenicactivityofstreptococcuspyogenesemm1andemm12genotypesisolatedfrompatientsandasymptomaticcarriers AT pvpigarevsky nephritogenicactivityofstreptococcuspyogenesemm1andemm12genotypesisolatedfrompatientsandasymptomaticcarriers AT vasnegova nephritogenicactivityofstreptococcuspyogenesemm1andemm12genotypesisolatedfrompatientsandasymptomaticcarriers AT evkuleshevich nephritogenicactivityofstreptococcuspyogenesemm1andemm12genotypesisolatedfrompatientsandasymptomaticcarriers AT dazharkov nephritogenicactivityofstreptococcuspyogenesemm1andemm12genotypesisolatedfrompatientsandasymptomaticcarriers AT cschalen nephritogenicactivityofstreptococcuspyogenesemm1andemm12genotypesisolatedfrompatientsandasymptomaticcarriers AT artematotolian nephritogenicactivityofstreptococcuspyogenesemm1andemm12genotypesisolatedfrompatientsandasymptomaticcarriers
_version_	1718418079923306496

NEPHRITOGENIC ACTIVITY OF STREPTOCOCCUS PYOGENES emm1 AND emm12 GENOTYPES ISOLATED FROM PATIENTS AND ASYMPTOMATIC CARRIERS

Ejemplares similares