Influence of Ageing on Vascular Reactivity and Receptor Expression in Rabbit Aorta: A Complement to Elastocalcinosis and Smooth Muscle Mechanisms

Nelson Ivan Cupitra, Juan C Calderón, Raul Narvaez-Sanchez Physiology and Biochemistry Research Group-PHYSIS, Faculty of Medicine, University of Antioquia, Medellin, ColombiaCorrespondence: Raul Narvaez-Sanchez Carrera 51 D #62-29, Office 203, Medellin, ColombiaTel +57 42196030Email raul....

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Autores principales:	Cupitra NI, Calderón JC, Narvaez-Sanchez R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	ageing aortic rings vascular reactivity vascular smooth muscle vascular stiffness Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/f6a1a3dd2f59428dbe5586ca6b8fab47
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spelling	oai:doaj.org-article:f6a1a3dd2f59428dbe5586ca6b8fab472021-12-02T09:24:07ZInfluence of Ageing on Vascular Reactivity and Receptor Expression in Rabbit Aorta: A Complement to Elastocalcinosis and Smooth Muscle Mechanisms1178-1998https://doaj.org/article/f6a1a3dd2f59428dbe5586ca6b8fab472020-04-01T00:00:00Zhttps://www.dovepress.com/influence-of-ageing-on-vascular-reactivity-and-receptor-expression-in--peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Nelson Ivan Cupitra, Juan C Calderón, Raul Narvaez-Sanchez Physiology and Biochemistry Research Group-PHYSIS, Faculty of Medicine, University of Antioquia, Medellin, ColombiaCorrespondence: Raul Narvaez-Sanchez Carrera 51 D #62-29, Office 203, Medellin, ColombiaTel +57 42196030Email raul.narvaez@udea.edu.coAim: To contribute to the knowledge about the mechanisms involved in aortic stiffness due to ageing.Materials and Methods: Aortic rings from young (1.5± 0.5 months, 0.8± 0.2 kg), adult (6± 0.5 months, 2.7± 0.5 kg) and old (28± 8 months, 3.2± 0.8 kg) male New Zealand rabbits were used to evaluate: 1) intima-media thickness by optical microscopy; 2) vascular reactivity (VR) in terms of sensitivity (pD2) and efficacy (Emax) to KCl; phenylephrine (PE); U-46619, a thromboxane A2 receptor agonist, TXA2; carbachol (CCh), isoproterenol and sodium nitroprusside (SNP), using organ bath experiments; and 3) the expression of receptors α 1, β 2 and thromboxane-prostanoids (TP), by immunofluorescence.Results: Ageing 1) did not change the thickness of tunica; 2) significantly reduced the pD2 to KCl, increased the pD2 to PE and reduced both the pD2 and Emax to TXA2, CCh and isoproterenol, and reduced the pD2 to SNP; and 3) significantly increased the expression of α 1 and β 2 receptors in the intima and adventitia, and the expression of TP only in the adventitia.Conclusion: Our results suggest that ageing makes the aorta more reactive to α 1 adrenergic contraction, and it could be a compensation for lower responsiveness to prostanoids. The aged aorta is less reactive to endothelium-dependent and non-dependent relaxation, and the vessel seems to try to compensate for that stiffness increasing β 2 receptors, although probably less functional. These results complement the proposed mechanisms of elastocalcinosis and smooth muscle rigidity, expanding the vision that should guide the treatment of aortic stiffness due to aging.Keywords: ageing, aortic rings, vascular reactivity, vascular smooth muscle, vascular stiffnessCupitra NICalderón JCNarvaez-Sanchez RDove Medical Pressarticleageingaortic ringsvascular reactivityvascular smooth musclevascular stiffnessGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 15, Pp 537-545 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	ageing aortic rings vascular reactivity vascular smooth muscle vascular stiffness Geriatrics RC952-954.6
spellingShingle	ageing aortic rings vascular reactivity vascular smooth muscle vascular stiffness Geriatrics RC952-954.6 Cupitra NI Calderón JC Narvaez-Sanchez R Influence of Ageing on Vascular Reactivity and Receptor Expression in Rabbit Aorta: A Complement to Elastocalcinosis and Smooth Muscle Mechanisms
description	Nelson Ivan Cupitra, Juan C Calderón, Raul Narvaez-Sanchez Physiology and Biochemistry Research Group-PHYSIS, Faculty of Medicine, University of Antioquia, Medellin, ColombiaCorrespondence: Raul Narvaez-Sanchez Carrera 51 D #62-29, Office 203, Medellin, ColombiaTel +57 42196030Email raul.narvaez@udea.edu.coAim: To contribute to the knowledge about the mechanisms involved in aortic stiffness due to ageing.Materials and Methods: Aortic rings from young (1.5± 0.5 months, 0.8± 0.2 kg), adult (6± 0.5 months, 2.7± 0.5 kg) and old (28± 8 months, 3.2± 0.8 kg) male New Zealand rabbits were used to evaluate: 1) intima-media thickness by optical microscopy; 2) vascular reactivity (VR) in terms of sensitivity (pD2) and efficacy (Emax) to KCl; phenylephrine (PE); U-46619, a thromboxane A2 receptor agonist, TXA2; carbachol (CCh), isoproterenol and sodium nitroprusside (SNP), using organ bath experiments; and 3) the expression of receptors α 1, β 2 and thromboxane-prostanoids (TP), by immunofluorescence.Results: Ageing 1) did not change the thickness of tunica; 2) significantly reduced the pD2 to KCl, increased the pD2 to PE and reduced both the pD2 and Emax to TXA2, CCh and isoproterenol, and reduced the pD2 to SNP; and 3) significantly increased the expression of α 1 and β 2 receptors in the intima and adventitia, and the expression of TP only in the adventitia.Conclusion: Our results suggest that ageing makes the aorta more reactive to α 1 adrenergic contraction, and it could be a compensation for lower responsiveness to prostanoids. The aged aorta is less reactive to endothelium-dependent and non-dependent relaxation, and the vessel seems to try to compensate for that stiffness increasing β 2 receptors, although probably less functional. These results complement the proposed mechanisms of elastocalcinosis and smooth muscle rigidity, expanding the vision that should guide the treatment of aortic stiffness due to aging.Keywords: ageing, aortic rings, vascular reactivity, vascular smooth muscle, vascular stiffness
format	article
author	Cupitra NI Calderón JC Narvaez-Sanchez R
author_facet	Cupitra NI Calderón JC Narvaez-Sanchez R
author_sort	Cupitra NI
title	Influence of Ageing on Vascular Reactivity and Receptor Expression in Rabbit Aorta: A Complement to Elastocalcinosis and Smooth Muscle Mechanisms
title_short	Influence of Ageing on Vascular Reactivity and Receptor Expression in Rabbit Aorta: A Complement to Elastocalcinosis and Smooth Muscle Mechanisms
title_full	Influence of Ageing on Vascular Reactivity and Receptor Expression in Rabbit Aorta: A Complement to Elastocalcinosis and Smooth Muscle Mechanisms
title_fullStr	Influence of Ageing on Vascular Reactivity and Receptor Expression in Rabbit Aorta: A Complement to Elastocalcinosis and Smooth Muscle Mechanisms
title_full_unstemmed	Influence of Ageing on Vascular Reactivity and Receptor Expression in Rabbit Aorta: A Complement to Elastocalcinosis and Smooth Muscle Mechanisms
title_sort	influence of ageing on vascular reactivity and receptor expression in rabbit aorta: a complement to elastocalcinosis and smooth muscle mechanisms
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/f6a1a3dd2f59428dbe5586ca6b8fab47
work_keys_str_mv	AT cupitrani influenceofageingonvascularreactivityandreceptorexpressioninrabbitaortaacomplementtoelastocalcinosisandsmoothmusclemechanisms AT calderonjc influenceofageingonvascularreactivityandreceptorexpressioninrabbitaortaacomplementtoelastocalcinosisandsmoothmusclemechanisms AT narvaezsanchezr influenceofageingonvascularreactivityandreceptorexpressioninrabbitaortaacomplementtoelastocalcinosisandsmoothmusclemechanisms
_version_	1718398160176414720

Influence of Ageing on Vascular Reactivity and Receptor Expression in Rabbit Aorta: A Complement to Elastocalcinosis and Smooth Muscle Mechanisms

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