3D GelMA ICC Scaffolds Combined with SW033291 for Bone Regeneration by Modulating Macrophage Polarization

Despite the interaction between bone marrow mesenchymal stem cells (BMSCs) and macrophages has been found to play a critical role in repairing bone defects, it remains a challenge to develop a desirable tissue engineering scaffold for synchronous regulation of osteogenic differentiation and macropha...

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Autores principales:	Qian Jiang, Guo Bai, Xin Liu, Yuxiao Chen, Guangzhou Xu, Chi Yang, Zhiyuan Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bone regeneration three-dimensional gelatin-methacryloyl inverted colloidal crystal (3D GelMA ICC) scaffold SW033291 M2 macrophage polarization bone marrow mesenchymal stem cells (BMSCs) Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/f6a1f86af7604743986990ac28d8895e
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spelling	oai:doaj.org-article:f6a1f86af7604743986990ac28d8895e2021-11-25T18:42:05Z3D GelMA ICC Scaffolds Combined with SW033291 for Bone Regeneration by Modulating Macrophage Polarization10.3390/pharmaceutics131119341999-4923https://doaj.org/article/f6a1f86af7604743986990ac28d8895e2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1934https://doaj.org/toc/1999-4923Despite the interaction between bone marrow mesenchymal stem cells (BMSCs) and macrophages has been found to play a critical role in repairing bone defects, it remains a challenge to develop a desirable tissue engineering scaffold for synchronous regulation of osteogenic differentiation and macrophage polarization. Herein, this study proposed a novel strategy to treat bone defects based on three-dimensional Gelatin Methacryloyl Inverted Colloidal Crystal (3D GelMA ICC) scaffold and an active 15-hydroxyprostaglandin dehydrogenase (15-PGDH) inhibitor SW033291. Specifically, the 3D GelMA ICC scaffolds were firstly prepared by colloidal templating method, which displayed good cell attachment and promoted intercellular interaction among macrophage and BMSCs due to its uniform pore interconnectivity. By combined use of SW033291, the release of Prostaglandin E2 (PGE2) from BMSCs on the GelMA ICC scaffold was significantly upregulated and macrophages M2 polarization was markedly increased. In turn, BMSCs proliferation and osteogenic differentiation was further enhanced by paracrine regulation of M2 macrophage, and thus finally caused more in vivo new bone formation by shaping up a pro-regenerative local immune microenvironment surrounding GelMA ICC scaffold. Our findings demonstrate the potential of 3D GelMA ICC scaffolds combined with SW033291 to become an effective tissue engineering strategy for bone regeneration.Qian JiangGuo BaiXin LiuYuxiao ChenGuangzhou XuChi YangZhiyuan ZhangMDPI AGarticlebone regenerationthree-dimensional gelatin-methacryloyl inverted colloidal crystal (3D GelMA ICC) scaffoldSW033291M2 macrophage polarizationbone marrow mesenchymal stem cells (BMSCs)Pharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1934, p 1934 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bone regeneration three-dimensional gelatin-methacryloyl inverted colloidal crystal (3D GelMA ICC) scaffold SW033291 M2 macrophage polarization bone marrow mesenchymal stem cells (BMSCs) Pharmacy and materia medica RS1-441
spellingShingle	bone regeneration three-dimensional gelatin-methacryloyl inverted colloidal crystal (3D GelMA ICC) scaffold SW033291 M2 macrophage polarization bone marrow mesenchymal stem cells (BMSCs) Pharmacy and materia medica RS1-441 Qian Jiang Guo Bai Xin Liu Yuxiao Chen Guangzhou Xu Chi Yang Zhiyuan Zhang 3D GelMA ICC Scaffolds Combined with SW033291 for Bone Regeneration by Modulating Macrophage Polarization
description	Despite the interaction between bone marrow mesenchymal stem cells (BMSCs) and macrophages has been found to play a critical role in repairing bone defects, it remains a challenge to develop a desirable tissue engineering scaffold for synchronous regulation of osteogenic differentiation and macrophage polarization. Herein, this study proposed a novel strategy to treat bone defects based on three-dimensional Gelatin Methacryloyl Inverted Colloidal Crystal (3D GelMA ICC) scaffold and an active 15-hydroxyprostaglandin dehydrogenase (15-PGDH) inhibitor SW033291. Specifically, the 3D GelMA ICC scaffolds were firstly prepared by colloidal templating method, which displayed good cell attachment and promoted intercellular interaction among macrophage and BMSCs due to its uniform pore interconnectivity. By combined use of SW033291, the release of Prostaglandin E2 (PGE2) from BMSCs on the GelMA ICC scaffold was significantly upregulated and macrophages M2 polarization was markedly increased. In turn, BMSCs proliferation and osteogenic differentiation was further enhanced by paracrine regulation of M2 macrophage, and thus finally caused more in vivo new bone formation by shaping up a pro-regenerative local immune microenvironment surrounding GelMA ICC scaffold. Our findings demonstrate the potential of 3D GelMA ICC scaffolds combined with SW033291 to become an effective tissue engineering strategy for bone regeneration.
format	article
author	Qian Jiang Guo Bai Xin Liu Yuxiao Chen Guangzhou Xu Chi Yang Zhiyuan Zhang
author_facet	Qian Jiang Guo Bai Xin Liu Yuxiao Chen Guangzhou Xu Chi Yang Zhiyuan Zhang
author_sort	Qian Jiang
title	3D GelMA ICC Scaffolds Combined with SW033291 for Bone Regeneration by Modulating Macrophage Polarization
title_short	3D GelMA ICC Scaffolds Combined with SW033291 for Bone Regeneration by Modulating Macrophage Polarization
title_full	3D GelMA ICC Scaffolds Combined with SW033291 for Bone Regeneration by Modulating Macrophage Polarization
title_fullStr	3D GelMA ICC Scaffolds Combined with SW033291 for Bone Regeneration by Modulating Macrophage Polarization
title_full_unstemmed	3D GelMA ICC Scaffolds Combined with SW033291 for Bone Regeneration by Modulating Macrophage Polarization
title_sort	3d gelma icc scaffolds combined with sw033291 for bone regeneration by modulating macrophage polarization
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f6a1f86af7604743986990ac28d8895e
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3D GelMA ICC Scaffolds Combined with SW033291 for Bone Regeneration by Modulating Macrophage Polarization

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